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Genome-wide comparative analysis of the BAHD superfamily in seven Rosaceae species and expression analysis in pear (Pyrus☓ bretschneideri)
Hao Yin
Nanjing Agricultural University
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Plant Biology.
Background: The BAHD acyltransferase superfamily exhibits various biological roles in plants, including regulating fruit quality, catalytic synthesizing of terpene, phenolics and esters, and improving stress resistance. However, the copy numbers, expression characteristics and associations with fruit aroma formation of the BAHD genes remain unclear. Results: In total, 717 BAHD genes were obtained from the genomes of seven Rosaceae , ( Pyrus bretschneideri , Malus domestica , Prunus avium , Prunus persica , Fragaria vesca , Pyrus communis and Rubus occidentalis ). Based on the detailed phylogenetic analysis and classifications in model plants, we divided the BAHD family genes into seven groups, I-a, I-b, II-a, II-b, III-a, IV and V. An inter-species synteny analysis revealed the ancient origin of BAHD superfamily with 78 syntenic gene pairs were detected among the seven Rosaceae species. Different types of gene duplication events jointly drive the expansion of BAHD superfamily, and purifying selection dominates the evolution of BAHD genes supported by the small Ka/Ks ratios . Based on the correlation analysis between the ester content and expression levels of BAHD genes at different developmental stages, four candidate genes were selected for verification as assessed by qRT-PCR. The result implied that Pbr020016.1 , Pbr019034.1 , Pbr014028.1 and Pbr029551.1 are important candidate genes involved in aroma formation during pear fruit development. Conclusion: We have thoroughly identified the BAHD superfamily genes and performed a comprehensive comparative analysis of their phylogenetic relationships, expansion patterns, and expression characteristics in seven Rosaceae species, and we also obtained four candidate genes involved in aroma synthesis in pear fruit . These results provide a theoretical basis for future studies of the specific biological functions of BAHD superfamily members and the improvement of pear fruit quality. Keywords : BAHD, pear, evolution, Rosaceae, transcriptome, volatile esters
Keywords
BAHD, pear, evolution, Rosaceae, transcriptome, volatile esters
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CURRENT STATUS: Published
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Posted 30 Dec, 2019
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On 08 Jan, 2020
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On 27 Dec, 2019
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Received 24 Nov, 2019
Editorial decision: Major revision
On 24 Nov, 2019
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Received 14 Nov, 2019
Reviewer #3 agreed
On 04 Nov, 2019
Reviewer #2 agreed
On 02 Nov, 2019
Review #2 received
Received 02 Nov, 2019
Reviewer #1 agreed
On 31 Oct, 2019
Editor assigned
On 30 Oct, 2019
Reviewers invited
Invitations sent on 30 Oct, 2019
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Editor invited
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Editorial decision: Major revision
On 05 Sep, 2019
Review #1 received
Received 02 Sep, 2019
Review #3 received
Received 02 Sep, 2019
Review #2 received
Received 31 Aug, 2019
Reviewer #3 agreed
On 19 Aug, 2019
Reviewer #2 agreed
On 18 Aug, 2019
Reviewer #1 agreed
On 18 Aug, 2019
Submission checks complete
On 16 Aug, 2019
Reviewers invited
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Editor assigned
On 08 Aug, 2019
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Genome-wide comparative analysis of the BAHD superfamily in seven Rosaceae species and expression analysis in pear (Pyrus☓ bretschneideri)
Hao Yin
Nanjing Agricultural University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 3
Posted 30 Dec, 2019
Published
On 08 Jan, 2020
No community comments so far
Editorial decision: Accept
On 27 Dec, 2019
Editor assigned
On 25 Dec, 2019
Submission checks complete
On 24 Dec, 2019
Editor invited
On 24 Dec, 2019
No comments provided
Review #3 received
Received 24 Nov, 2019
Editorial decision: Major revision
On 24 Nov, 2019
Review #1 received
Received 14 Nov, 2019
Reviewer #3 agreed
On 04 Nov, 2019
Reviewer #2 agreed
On 02 Nov, 2019
Review #2 received
Received 02 Nov, 2019
Reviewer #1 agreed
On 31 Oct, 2019
Editor assigned
On 30 Oct, 2019
Reviewers invited
Invitations sent on 30 Oct, 2019
Submission checks complete
On 29 Oct, 2019
Editor invited
On 29 Oct, 2019
No comments provided
Editorial decision: Major revision
On 05 Sep, 2019
Review #1 received
Received 02 Sep, 2019
Review #3 received
Received 02 Sep, 2019
Review #2 received
Received 31 Aug, 2019
Reviewer #3 agreed
On 19 Aug, 2019
Reviewer #2 agreed
On 18 Aug, 2019
Reviewer #1 agreed
On 18 Aug, 2019
Submission checks complete
On 16 Aug, 2019
Reviewers invited
Invitations sent on 16 Aug, 2019
Editor assigned
On 08 Aug, 2019
Editor invited
On 07 Aug, 2019
First submitted
On 06 Aug, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Plant Biology.
Background: The BAHD acyltransferase superfamily exhibits various biological roles in plants, including regulating fruit quality, catalytic synthesizing of terpene, phenolics and esters, and improving stress resistance. However, the copy numbers, expression characteristics and associations with fruit aroma formation of the BAHD genes remain unclear. Results: In total, 717 BAHD genes were obtained from the genomes of seven Rosaceae , ( Pyrus bretschneideri , Malus domestica , Prunus avium , Prunus persica , Fragaria vesca , Pyrus communis and Rubus occidentalis ). Based on the detailed phylogenetic analysis and classifications in model plants, we divided the BAHD family genes into seven groups, I-a, I-b, II-a, II-b, III-a, IV and V. An inter-species synteny analysis revealed the ancient origin of BAHD superfamily with 78 syntenic gene pairs were detected among the seven Rosaceae species. Different types of gene duplication events jointly drive the expansion of BAHD superfamily, and purifying selection dominates the evolution of BAHD genes supported by the small Ka/Ks ratios . Based on the correlation analysis between the ester content and expression levels of BAHD genes at different developmental stages, four candidate genes were selected for verification as assessed by qRT-PCR. The result implied that Pbr020016.1 , Pbr019034.1 , Pbr014028.1 and Pbr029551.1 are important candidate genes involved in aroma formation during pear fruit development. Conclusion: We have thoroughly identified the BAHD superfamily genes and performed a comprehensive comparative analysis of their phylogenetic relationships, expansion patterns, and expression characteristics in seven Rosaceae species, and we also obtained four candidate genes involved in aroma synthesis in pear fruit . These results provide a theoretical basis for future studies of the specific biological functions of BAHD superfamily members and the improvement of pear fruit quality. Keywords : BAHD, pear, evolution, Rosaceae, transcriptome, volatile esters
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