First Record of Migonemyia Vaniae (Galati, Fonseca & Marassá, 2007) (Diptera, Psychodidae, Phlebotominae) in the State of Rio De Janeiro.


 Entomological surveillance studies in areas that have not yet been evaluated become important for the survey of local fauna, where new taxons or species records can be observed. In an investigation carried out at the Jequitibá headquarters of the Três Picos State Park, the presence of the species Migonemyia vaniae was observed, until then described and recorded only in the state of São Paulo, thus, we report the first encounter of the species in the state of Rio de Janeiro. Two male specimens of Mg vaniae with CDC light traps (HP model) were captured. Specimens of Mg vaniae and Migonemyia migonei were submitted to the clarification process and mounted between slide and coverslip in Berlese and identified under microscope following the Galati classification. Morphometric analyses of the ejaculatory pump and edeagais ducts and photographs of the structures were performed in order to compare the differences between species and record the first report of the species Mg vaniae in the state of Rio de Janeiro.


Introduction
The sand ies are dipterous insects of the Psychodidae family and subfamily Phlebotominae, presenting preferentially nocturnal twilight habits and are the vectors of Leishmania spp. (Rangel and Lainson, 2003).
Currently, 1026 taxa of the species group are described worldwide, being 985 valid and 32 fossils, and in the American Continent there are 546 species, 529 current and 17 fossils (Galati, 2019), where about 10% of these species may be involved in the transmission of human pathogens ( The present work aims to report the rst record of the specie Migonemyia vaniae (Galati, Fonseca & Marassá, 2007), in the state of Rio de Janeiro, a species described during collections carried out in the rural area of the state of São Paulo in the Ribeira Valley, near the reserve in the Serra de Paranapiacaba (Galati et al. 2007). The species Migonemyia vaniae differs Migonemyia rabelloi (Galati & Gomes, 1992) and Migonemyia migonei (França, 1920), by presenting the length of the ejaculatory pump and the larger edeagal ducts in relation to the other species. Another important characteristic is the concavity formed by the paramer in its distal margin (Galati et al. 2007). Characteristics that should be carefully analyzed through morphometric analyses of the structures to con rm taxonomic identi cation. To con rm the species of rst record in the state of Rio de Janeiro, the slides containing the specimens of Migonenyia vaniae and migonemyia migonei specimens were photographed under optical microscope (PrimoStar, Carl Zeiss®), connected to the AxioCam camera (Carl Zeiss®). Imaging Systems 4.7.2 (Carl Zeiss® was used to perform measurements of the ejaculatory pump and edeagais in order to compare the two species of the genus.

Results And Discussion
After twelve months of capture of sand y, two male specimens diagnosed as Mg vaniae were collected, a species that had not been recorded until then in the state of Rio de Janeiro. One specimen was collected in Ambiente, 2020). These factors help to understanding the occurrence of the species in these localities. However, regarding altitude, PETP EM4 is at 464m sea level, while the meeting point of the species in the Ribeira valley is 260m above sea level, suggesting a variation in altitude of possible encounter of this species in biotopes similar to those described in these reports.
By performing the taxonomic identi cation of the specimens of Mg migonei and Mg vanie, it is possible to observe the two species presenting short IF smaller than the halves of the head length ( Fig. 1A and 1B) and gonotile with the internal spine implanted very close to the apex (Fig. 1C and 1D), as described in the dichotomous key of Galati (2003Galati ( , 2019, characteristic of species belonging to the genus Migonemyia spp. Analyzing the two specimens of Mg vaniae we can observe the paramer in digitiform format and its dorsal margin slightly convex (Fig. 1C), while the specimens of Mg migonei present this paramer strongly convex in the dorsal margin (Fig. 1D). As shown in the original description of the species (Galati et al. 2007).
The con rmation of the presence of the species was carried out through the morphometry of the edeagal ducts of the species, characteristics pointed out in the description by Galati et al (2007). Thus, it was possible to observe that the two specimens of Mg vaniae had an average of 145.803 mm in spermatic pump length and 646.373 mm length of the edeagais duct, and the mean obtained after the morometry of four specimens of Mg migonei was 122.397mm and 545.006 respectively (Table I). These data corroborate the data described by Galati et al. 2007, which de nes the difference between the species Mg vaniae and Mg migonei as follows, the latter presents pairâmer strongly convex in the dorsal margin between two concavities and ejaculatory ducts and ejaculatory pump measuring ≤ 590 mm and