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Shan Gao
Beijing University of Agriculture
Corresponding Author
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Background: Natural astaxanthin (ASTA) has strong antioxidant properties and has been widely used as a health product to improve human health. However, the effects of ASTA on the reproductive performance of aging roosters have been poorly studied. We aimed to investigate the effects of dietary ASTA on semen quality and antioxidant capacity in aging roosters and to explore the potential mechanism of semen quality change via anti-oxidation defense system.
Methods: In the present study, 96 53-week-old Jinghong No. 1 layer breeder roosters were fed a corn-soybean meal basal diet containing ASTA at 0, 25, 50, or 100 mg/kg for 6 week.
Results: Semen quality in the ASTA groups remarkably improved as compared to those in the control group and antioxidant activities, the abilities to scavenge hydroxyl radicals and superoxide anions increased gradually with ASTA addition (P < 0.05). In addition, the mRNA levels of antioxidant enzymes, the mRNA and protein levels of the mitogen-activated protein kinase (MAPK), nuclear factor-erythroid 2-related factor 2 (Nrf2) were markedly increased in the 50-100 mg/kg ASTA group (P < 0.05).
Conclusions: Collectively, these results demonstrate that dietary ASTA may improve semen quality by increasing antioxidant enzyme activities, and the ability to scavenge hydroxyl radicals, which may be related to up-regulation of the MAPK/Nrf2 pathway.
Keywords
natural astaxanthin, semen quality, antioxidant capacity, MAPK/Nrf2 pathway, aging rooster
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CURRENT STATUS: Under Review
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Posted 13 Apr, 2021
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Editorial decision: Major revision
On 06 Jul, 2021
Reviewer #4 agreed
On 21 Jun, 2021
Review #1 received
Received 13 Jun, 2021
Review #2 received
Received 13 Jun, 2021
Reviewer #3 agreed
On 12 Jun, 2021
Reviews received
Received 22 May, 2021
Reviewer #2 agreed
On 21 May, 2021
Reviewer #1 agreed
On 08 May, 2021
Reviewers invited
Invitations sent on 12 Apr, 2021
First submitted
On 07 Apr, 2021
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This preprint is under consideration at Journal of Animal Science and Biotechnology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Shan Gao
Beijing University of Agriculture
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 13 Apr, 2021
No community comments so far
Editorial decision: Major revision
On 06 Jul, 2021
Reviewer #4 agreed
On 21 Jun, 2021
Review #1 received
Received 13 Jun, 2021
Review #2 received
Received 13 Jun, 2021
Reviewer #3 agreed
On 12 Jun, 2021
Reviews received
Received 22 May, 2021
Reviewer #2 agreed
On 21 May, 2021
Reviewer #1 agreed
On 08 May, 2021
Reviewers invited
Invitations sent on 12 Apr, 2021
First submitted
On 07 Apr, 2021
Editor assigned
On 07 Apr, 2021
Submission checks complete
On 07 Apr, 2021
Editor invited
On 07 Apr, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Natural astaxanthin (ASTA) has strong antioxidant properties and has been widely used as a health product to improve human health. However, the effects of ASTA on the reproductive performance of aging roosters have been poorly studied. We aimed to investigate the effects of dietary ASTA on semen quality and antioxidant capacity in aging roosters and to explore the potential mechanism of semen quality change via anti-oxidation defense system.
Methods: In the present study, 96 53-week-old Jinghong No. 1 layer breeder roosters were fed a corn-soybean meal basal diet containing ASTA at 0, 25, 50, or 100 mg/kg for 6 week.
Results: Semen quality in the ASTA groups remarkably improved as compared to those in the control group and antioxidant activities, the abilities to scavenge hydroxyl radicals and superoxide anions increased gradually with ASTA addition (P < 0.05). In addition, the mRNA levels of antioxidant enzymes, the mRNA and protein levels of the mitogen-activated protein kinase (MAPK), nuclear factor-erythroid 2-related factor 2 (Nrf2) were markedly increased in the 50-100 mg/kg ASTA group (P < 0.05).
Conclusions: Collectively, these results demonstrate that dietary ASTA may improve semen quality by increasing antioxidant enzyme activities, and the ability to scavenge hydroxyl radicals, which may be related to up-regulation of the MAPK/Nrf2 pathway.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
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