This study aimed to assess the effect of drying unbleached Eucalyptus cellulose fibers after the application of pretreatments in order to optimize the microfibrillation process, as well as to evaluate the efficiency of NaOH pretreatments in reducing energy consumption for production of microfibrillated cellulose (MFC). Pretreatments with 0 wt% (untreated), 5 wt% and 10 wt% NaOH were evaluated. The length and width of the fibers pretreated with NaOH decreased significantly, mainly with hasher pretreatments. The removal of hemicellulose from the fiber cell wall was an important factor concerning the degree of fibrillation of the fibers. Pretreating fibers with 5 wt% NaOH for 2 h increased the water retention value (WRV), in addition to presenting the lowest energy consumption for fibrillation, promoting energy savings of up to 48%. Pulps that were not dried after the NaOH pretreatments incurred in easier microfibrillation and lower energy consumption when comparing to the dried pulp, which shows the negative impact of drying on the fibers to obtain the MFC.