See the published version of this preprint at BMC Plant Biology.
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Research article
Zhiyong Wang
Hainan University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Highlights:
1. Axonopus compressus can stand severe drought stress by activating the potential defense mechanism.
2. We investigated the differential transcriptome of drought stressed and normal Axonopus compressus plants
3. New comers have been identified that involved in the drought response
4. Identified drought responsive genes which never known for other stresses.
5. The identified genes also respond to stress in Arabidopsis thaliana in different manners.
Background: Carpet grass [Axonopus compressus (L.)] is an important warm season perennial grass around the world and is renowned for its adaptability to varied environmental conditions. However, Carpet grass lacks enough data in public data bank, which confined our comprehension of the mechanism of environmental adaptations, gene discovery and development of molecular marker.
Methods: In current study, the DEGs (differentially expressed genes) in Axonopus compressus under drought stress (DS) were identified and compared with CK (control) by RNA-Seq.
Results: the 263,835 of total unigenes were identified in Axonopus compressus, and 201,303 (also add the numbers of remaining2 data bases) a sequence of unigenes significantly matched in at least one of the seven databases. A total of 153697 (58.25%) unigenes can be classified to 144 KEGG pathways, and 7,444 unigenes were expressed differentially between DS and CK, of which 4,249 were up-regulated and 3,195 were down-regulated unigenes. Of the 50 significantly enriched GO terms, 18, 6 and 14 items were related to BP, CC and MF, respectively. Analysis of KEGG enrichment showed 2569 DEGs involved in 143 different pathways, under drought stress 2,747 DEGs were up-regulated and 2,502 DEGs were down-regulated. Moreover, we identified 352 transcriptor factors (TFs) in Axonopus compressus, of which 270 were differentially expressed between CK and DS. The qRT-PCR validation experiment also support the transcriptional response of Axonopus compressus against drought.
Conclusions: The current findings provide the first framework for further investigation for the particular roles of these unigenes in Axonopus compressus in response to droughts.
Keywords
Drought, Axonopus compressus, qRT-PCR, Enrichment analysis, lncRNAs, miRNAs, Transcriptomics
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This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.Metabolicpathwayanalysiskegg.xlsx
Metabolic pathway analysis keg (XLS 45.0 Kb)
- Additionalfile2.Differentially.xlsx
Differentially expressed gene (XLS 1.85 Mb)
- Additionalfile3.GOclassification.xlsx
GO classification (XLS 148 Kb)
- Additionalfile4.GOenrichment.xlsx
GO enrichment (XLS 380 Kb)
- Additionalfile5.KEGGpathway.xlsx
KEGGpathway (XLS 388 Kb)
- Additionalfile6.Transcriptionfactors.docx
Transcription factors (DOC 16 Kb)
- Additionalfile7.Transcription.docx
Transcription regulatory factors (DOC 16 Kb)
- Additionalfile8.Listoftheprimersused.docx
List of the primers used for the qRT-PCR (DOC 15.1 Kb)
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CURRENT STATUS: Published
View the published article at BMC Plant Biology.
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On 31 Dec, 2020
Editorial decision: Minor revision
On 29 Dec, 2020
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Editorial decision: Minor revision
On 23 Dec, 2020
Review #2 received
Received 02 Dec, 2020
Review #1 received
Received 02 Dec, 2020
Reviewer #2 agreed
On 30 Nov, 2020
Editor assigned
On 30 Nov, 2020
Reviewers invited
Invitations sent on 30 Nov, 2020
Reviewer #1 agreed
On 30 Nov, 2020
Submission checks complete
On 30 Nov, 2020
Editor invited
On 30 Nov, 2020
No comments provided
Editorial decision: Minor revision
On 15 Nov, 2020
Review #1 received
Received 28 Oct, 2020
Review #2 received
Received 27 Oct, 2020
Reviewers invited
Invitations sent on 20 Oct, 2020
Reviewer #1 agreed
On 20 Oct, 2020
Reviewer #2 agreed
On 20 Oct, 2020
Editor assigned
On 13 Oct, 2020
Submission checks complete
On 12 Oct, 2020
Editor invited
On 12 Oct, 2020
Version 1
Posted 23 Jul, 2020
No comments provided
Editorial decision: Major revision
On 31 Aug, 2020
Review #1 received
Received 21 Aug, 2020
Review #2 received
Received 21 Aug, 2020
Reviewer #3 agreed
On 04 Aug, 2020
Reviewers invited
Invitations sent on 03 Aug, 2020
Reviewer #1 agreed
On 03 Aug, 2020
Reviewer #2 agreed
On 03 Aug, 2020
Editor assigned
On 15 Jul, 2020
First submitted
On 14 Jul, 2020
Submission checks complete
On 14 Jul, 2020
Editor invited
On 14 Jul, 2020
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Plant Biology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Zhiyong Wang
Hainan University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Plant Biology.
No community comments so far
Submission checks complete
On 31 Dec, 2020
Editorial decision: Minor revision
On 29 Dec, 2020
No comments provided
Editorial decision: Minor revision
On 23 Dec, 2020
Review #2 received
Received 02 Dec, 2020
Review #1 received
Received 02 Dec, 2020
Reviewer #2 agreed
On 30 Nov, 2020
Editor assigned
On 30 Nov, 2020
Reviewers invited
Invitations sent on 30 Nov, 2020
Reviewer #1 agreed
On 30 Nov, 2020
Submission checks complete
On 30 Nov, 2020
Editor invited
On 30 Nov, 2020
No comments provided
Editorial decision: Minor revision
On 15 Nov, 2020
Review #1 received
Received 28 Oct, 2020
Review #2 received
Received 27 Oct, 2020
Reviewers invited
Invitations sent on 20 Oct, 2020
Reviewer #1 agreed
On 20 Oct, 2020
Reviewer #2 agreed
On 20 Oct, 2020
Editor assigned
On 13 Oct, 2020
Submission checks complete
On 12 Oct, 2020
Editor invited
On 12 Oct, 2020
Version 1
Posted 23 Jul, 2020
No comments provided
Editorial decision: Major revision
On 31 Aug, 2020
Review #1 received
Received 21 Aug, 2020
Review #2 received
Received 21 Aug, 2020
Reviewer #3 agreed
On 04 Aug, 2020
Reviewers invited
Invitations sent on 03 Aug, 2020
Reviewer #1 agreed
On 03 Aug, 2020
Reviewer #2 agreed
On 03 Aug, 2020
Editor assigned
On 15 Jul, 2020
First submitted
On 14 Jul, 2020
Submission checks complete
On 14 Jul, 2020
Editor invited
On 14 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Plant Biology.
Highlights:
1. Axonopus compressus can stand severe drought stress by activating the potential defense mechanism.
2. We investigated the differential transcriptome of drought stressed and normal Axonopus compressus plants
3. New comers have been identified that involved in the drought response
4. Identified drought responsive genes which never known for other stresses.
5. The identified genes also respond to stress in Arabidopsis thaliana in different manners.
Background: Carpet grass [Axonopus compressus (L.)] is an important warm season perennial grass around the world and is renowned for its adaptability to varied environmental conditions. However, Carpet grass lacks enough data in public data bank, which confined our comprehension of the mechanism of environmental adaptations, gene discovery and development of molecular marker.
Methods: In current study, the DEGs (differentially expressed genes) in Axonopus compressus under drought stress (DS) were identified and compared with CK (control) by RNA-Seq.
Results: the 263,835 of total unigenes were identified in Axonopus compressus, and 201,303 (also add the numbers of remaining2 data bases) a sequence of unigenes significantly matched in at least one of the seven databases. A total of 153697 (58.25%) unigenes can be classified to 144 KEGG pathways, and 7,444 unigenes were expressed differentially between DS and CK, of which 4,249 were up-regulated and 3,195 were down-regulated unigenes. Of the 50 significantly enriched GO terms, 18, 6 and 14 items were related to BP, CC and MF, respectively. Analysis of KEGG enrichment showed 2569 DEGs involved in 143 different pathways, under drought stress 2,747 DEGs were up-regulated and 2,502 DEGs were down-regulated. Moreover, we identified 352 transcriptor factors (TFs) in Axonopus compressus, of which 270 were differentially expressed between CK and DS. The qRT-PCR validation experiment also support the transcriptional response of Axonopus compressus against drought.
Conclusions: The current findings provide the first framework for further investigation for the particular roles of these unigenes in Axonopus compressus in response to droughts.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.Metabolicpathwayanalysiskegg.xlsx
Metabolic pathway analysis keg (XLS 45.0 Kb)
- Additionalfile2.Differentially.xlsx
Differentially expressed gene (XLS 1.85 Mb)
- Additionalfile3.GOclassification.xlsx
GO classification (XLS 148 Kb)
- Additionalfile4.GOenrichment.xlsx
GO enrichment (XLS 380 Kb)
- Additionalfile5.KEGGpathway.xlsx
KEGGpathway (XLS 388 Kb)
- Additionalfile6.Transcriptionfactors.docx
Transcription factors (DOC 16 Kb)
- Additionalfile7.Transcription.docx
Transcription regulatory factors (DOC 16 Kb)
- Additionalfile8.Listoftheprimersused.docx
List of the primers used for the qRT-PCR (DOC 15.1 Kb)
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