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Research
Qi Shi
Baoshan Branch, Shuguang Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8743-1134
License:
This work is licensed under a CC BY 4.0 License. Read Full License
OBJECTIVE
To identify the effect of traditional Chinese medicine (TCM) on postoperative patients with stage II-III colorectal cancer (CRC) and to explore the core herb combination and its mechanism.
METHODS
For this observational cohort study, patients diagnosed between January 2006 and September 2016 were enrolled from two hospitals, data was followed up from January 2019 to November 2020 and was analyzed in December 2020. The primary outcome, disease-free survival (DFS), was compared for those patients who received or did not receive TCM, and the secondary outcome was the hazard ratio (HR). The relevance principle was used to obtain the candidate herb combinations, and the core combination was evaluated through assessment of efficacy and representativeness. Then, the interactions among herbs and CRC were visualized by Cytoscape. Furthermore, related biological processes and signalling pathways were obtained by enrichment and KEGG analyses.
RESULTS
A total of 707 patients were finally included. In general, TCM decreased the metastatic recurrence associated with stage II-III CRC (HR: 0.61, log-rank P＜0.05). Among those patients in the TCM group, the core combination was Baizhu → Yinchen, Chenpi, and Fuling (C), and its antitumor mechanism was most likely related to 30 core targets, which were associated with 327 biological processes and 111 KEGG pathways. The most closely associated pathways were the PI3K-Akt, MAPK and HIF-1 pathways.
CONCLUSIONS
On the whole, a combination of four herbs, Baizhu → Yinchen, Chenpi, and Fuling, could reduce metastatic recurrence in postoperative patients with stage II-III CRC, and the mechanism might be related to the PI3K-Akt, MAPK and HIF-1 pathways.
Keywords
Chinese herbal formula, colorectal cancer, network pharmacology
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Qi Shi
Baoshan Branch, Shuguang Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8743-1134
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 04 May, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Internal Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
OBJECTIVE
To identify the effect of traditional Chinese medicine (TCM) on postoperative patients with stage II-III colorectal cancer (CRC) and to explore the core herb combination and its mechanism.
METHODS
For this observational cohort study, patients diagnosed between January 2006 and September 2016 were enrolled from two hospitals, data was followed up from January 2019 to November 2020 and was analyzed in December 2020. The primary outcome, disease-free survival (DFS), was compared for those patients who received or did not receive TCM, and the secondary outcome was the hazard ratio (HR). The relevance principle was used to obtain the candidate herb combinations, and the core combination was evaluated through assessment of efficacy and representativeness. Then, the interactions among herbs and CRC were visualized by Cytoscape. Furthermore, related biological processes and signalling pathways were obtained by enrichment and KEGG analyses.
RESULTS
A total of 707 patients were finally included. In general, TCM decreased the metastatic recurrence associated with stage II-III CRC (HR: 0.61, log-rank P＜0.05). Among those patients in the TCM group, the core combination was Baizhu → Yinchen, Chenpi, and Fuling (C), and its antitumor mechanism was most likely related to 30 core targets, which were associated with 327 biological processes and 111 KEGG pathways. The most closely associated pathways were the PI3K-Akt, MAPK and HIF-1 pathways.
CONCLUSIONS
On the whole, a combination of four herbs, Baizhu → Yinchen, Chenpi, and Fuling, could reduce metastatic recurrence in postoperative patients with stage II-III CRC, and the mechanism might be related to the PI3K-Akt, MAPK and HIF-1 pathways.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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