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Research article
Alireza Pasdar
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7864-9729
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Background: Genetic factors play an important role in susceptibility to methamphetamine. In this line, protein that interact with C-kinase-1 (PICK1) and brain-derived neurotrophic factor (BDNF) genes are linked to methamphetamine dependence (substance use disorder). Thus, in a case-control study, we investigated the association between polymorphisms of PICK1 and BDNF genes and methamphetamine dependence in an Iranian population from 2015 to 2018.
Methods: Total of 235 cases and 204 controls were recruited. The PICK1-rs713729, -rs2076369 and BDNF-rs6265 genotypes were determined via ARMS-PCR assay. Statistical analysis was performed, using SPSS 20.0, PHASE 2.1.1 program as well as SNP Analyzer 2.0.
Results: In the present study, two polymorphisms including PICK1-rs713729 (OR: 1.38 (CI: 1.08-1.52; P-value: 0.004) in multiplicative and dominant models, and PICK1-rs2076369 (OR: 1.31 (CI: 1.10-1.56; P-value: 0.002) in multiplicative, dominant and co-dominant models were associated with the risk of methamphetamine abuse. Moreover, haplotype analysis methods showed a significant association between haplotype AG (OR: 2.50 (CI: 1.50-4.16; P-value: 0.0002) in dominant, recessive and co-dominant models, and haplotype TT (OR: 0.67 (CI: 0.50-0.91; P-value: 0.009) in dominant model and co-dominant model with the risk of methamphetamine abuse. None of the polymorphisms in this study had a high level of linkage disequilibrium.
Conclusion: Our findings indicate that the PICK1 gene might be linked to methamphetamine dependence. Therefore, it can be stated that PICK1 might play a significant role in the pathophysiology of methamphetamine dependence in an Iranian population.
Keywords
Addiction, drug abuse, substance dependence, substance use disorder (SUD), dopamine pathway, glutamate pathway, variations, and polymorphisms
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Alireza Pasdar
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7864-9729
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
The most recent version of this article is available here.
No community comments so far
No comments provided
Version 1
Posted 20 Jul, 2020
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Medical Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The most recent version of this article is available here.
Background: Genetic factors play an important role in susceptibility to methamphetamine. In this line, protein that interact with C-kinase-1 (PICK1) and brain-derived neurotrophic factor (BDNF) genes are linked to methamphetamine dependence (substance use disorder). Thus, in a case-control study, we investigated the association between polymorphisms of PICK1 and BDNF genes and methamphetamine dependence in an Iranian population from 2015 to 2018.
Methods: Total of 235 cases and 204 controls were recruited. The PICK1-rs713729, -rs2076369 and BDNF-rs6265 genotypes were determined via ARMS-PCR assay. Statistical analysis was performed, using SPSS 20.0, PHASE 2.1.1 program as well as SNP Analyzer 2.0.
Results: In the present study, two polymorphisms including PICK1-rs713729 (OR: 1.38 (CI: 1.08-1.52; P-value: 0.004) in multiplicative and dominant models, and PICK1-rs2076369 (OR: 1.31 (CI: 1.10-1.56; P-value: 0.002) in multiplicative, dominant and co-dominant models were associated with the risk of methamphetamine abuse. Moreover, haplotype analysis methods showed a significant association between haplotype AG (OR: 2.50 (CI: 1.50-4.16; P-value: 0.0002) in dominant, recessive and co-dominant models, and haplotype TT (OR: 0.67 (CI: 0.50-0.91; P-value: 0.009) in dominant model and co-dominant model with the risk of methamphetamine abuse. None of the polymorphisms in this study had a high level of linkage disequilibrium.
Conclusion: Our findings indicate that the PICK1 gene might be linked to methamphetamine dependence. Therefore, it can be stated that PICK1 might play a significant role in the pathophysiology of methamphetamine dependence in an Iranian population.
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