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Short report
Guo-Hua Liu
Hunan Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8434-899X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
The family Hoplopleuridae contains at least 183 species of blood-sucking lice, which widely parasitize both mice and rats. Fragmented mitochondrial (mt) genomes have been reported in two rat lice ( Hoplopleura kitti and H. akanezumi ) from this family, but some minichromosomes were unidentified in their mt genomes.
Methods
We sequenced the mt genome of rat louse Hoplopleura sp. with an Illumina Hiseq platform and compared its mt genome organization with H. kitti and H. akanezumi .
Results
Fragmented mt genome of the rat louse Hoplopleura sp. contains 37 genes which are on 12 circular mt minichromosomes. Each mt minichromosome is 1.8-2.7 kb long, which contains 1-5 genes and one large non-coding region. The gene content and arrangement of three mt minichromosomes of Hoplopleura sp. and H. kitti are different from that of the three mt minichromosomes of H. akanezumi . Phylogenetic analyses based on the deduced amino acid sequences of the eight protein-coding genes showed that the Hoplopleura sp. was more closely related to H. akanezumi than to H. kitti , and then they form a monophyletic group.
Conclusions
Comparison among the three rat lice revealed variation in the composition of mt minichromosomes within the genus Hoplopleura . Hoplopleura sp. is the first species from the family Hoplopleuridae for which a complete fragmented mt genome has been sequenced. The new data provides useful genetic markers for studying the population genetics, molecular systematics and phylogenetics of blood-sucking lice.
Keywords
Rat louse, Fragmented mt genome, Phylogenetic analyses
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Peer Review Timeline
CURRENT STATUS: Published
View the published article at Parasites & Vectors.
No community comments so far
Editor assigned
On 24 Sep, 2020
Editorial decision: Accept
On 24 Sep, 2020
Submission checks complete
On 23 Sep, 2020
Editor invited
On 23 Sep, 2020
No comments provided
Editor assigned
On 11 Sep, 2020
Submission checks complete
On 10 Sep, 2020
Editor invited
On 10 Sep, 2020
Version 3
Posted 10 Sep, 2020
No comments provided
Reviewer #1 agreed
On 09 Sep, 2020
Review #1 received
Received 09 Sep, 2020
Editorial decision: Minor Revision
On 09 Sep, 2020
Editor assigned
On 07 Sep, 2020
Reviewers invited
Invitations sent on 07 Sep, 2020
Submission checks complete
On 06 Sep, 2020
Editor invited
On 06 Sep, 2020
No comments provided
Editorial decision: Major Revision
On 01 Sep, 2020
Review #2 received
Received 31 Aug, 2020
Review #1 received
Received 27 Aug, 2020
Reviewer #2 agreed
On 25 Aug, 2020
Editor assigned
On 22 Aug, 2020
Reviewers invited
Invitations sent on 22 Aug, 2020
Reviewer #1 agreed
On 22 Aug, 2020
Submission checks complete
On 21 Aug, 2020
Editor invited
On 21 Aug, 2020
No comments provided
Editorial decision: Major Revision
On 31 Jul, 2020
Review #1 received
Received 30 Jul, 2020
Review #2 received
Received 27 Jul, 2020
Reviewer #2 agreed
On 18 Jul, 2020
Reviewer #1 agreed
On 17 Jul, 2020
Reviewers invited
Invitations sent on 15 Jul, 2020
Editor invited
On 13 Jul, 2020
Editor assigned
On 13 Jul, 2020
First submitted
On 12 Jul, 2020
Submission checks complete
On 12 Jul, 2020
Metrics
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PDF Downloads: ...
HTML Views: ...
Subject Areas
Parasitology
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Parasites & Vectors.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Short report
Guo-Hua Liu
Hunan Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8434-899X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Parasites & Vectors.
No community comments so far
Editor assigned
On 24 Sep, 2020
Editorial decision: Accept
On 24 Sep, 2020
Submission checks complete
On 23 Sep, 2020
Editor invited
On 23 Sep, 2020
No comments provided
Editor assigned
On 11 Sep, 2020
Submission checks complete
On 10 Sep, 2020
Editor invited
On 10 Sep, 2020
Version 3
Posted 10 Sep, 2020
No comments provided
Reviewer #1 agreed
On 09 Sep, 2020
Review #1 received
Received 09 Sep, 2020
Editorial decision: Minor Revision
On 09 Sep, 2020
Editor assigned
On 07 Sep, 2020
Reviewers invited
Invitations sent on 07 Sep, 2020
Submission checks complete
On 06 Sep, 2020
Editor invited
On 06 Sep, 2020
No comments provided
Editorial decision: Major Revision
On 01 Sep, 2020
Review #2 received
Received 31 Aug, 2020
Review #1 received
Received 27 Aug, 2020
Reviewer #2 agreed
On 25 Aug, 2020
Editor assigned
On 22 Aug, 2020
Reviewers invited
Invitations sent on 22 Aug, 2020
Reviewer #1 agreed
On 22 Aug, 2020
Submission checks complete
On 21 Aug, 2020
Editor invited
On 21 Aug, 2020
No comments provided
Editorial decision: Major Revision
On 31 Jul, 2020
Review #1 received
Received 30 Jul, 2020
Review #2 received
Received 27 Jul, 2020
Reviewer #2 agreed
On 18 Jul, 2020
Reviewer #1 agreed
On 17 Jul, 2020
Reviewers invited
Invitations sent on 15 Jul, 2020
Editor invited
On 13 Jul, 2020
Editor assigned
On 13 Jul, 2020
First submitted
On 12 Jul, 2020
Submission checks complete
On 12 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Parasitology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Parasites & Vectors.
Background
The family Hoplopleuridae contains at least 183 species of blood-sucking lice, which widely parasitize both mice and rats. Fragmented mitochondrial (mt) genomes have been reported in two rat lice ( Hoplopleura kitti and H. akanezumi ) from this family, but some minichromosomes were unidentified in their mt genomes.
Methods
We sequenced the mt genome of rat louse Hoplopleura sp. with an Illumina Hiseq platform and compared its mt genome organization with H. kitti and H. akanezumi .
Results
Fragmented mt genome of the rat louse Hoplopleura sp. contains 37 genes which are on 12 circular mt minichromosomes. Each mt minichromosome is 1.8-2.7 kb long, which contains 1-5 genes and one large non-coding region. The gene content and arrangement of three mt minichromosomes of Hoplopleura sp. and H. kitti are different from that of the three mt minichromosomes of H. akanezumi . Phylogenetic analyses based on the deduced amino acid sequences of the eight protein-coding genes showed that the Hoplopleura sp. was more closely related to H. akanezumi than to H. kitti , and then they form a monophyletic group.
Conclusions
Comparison among the three rat lice revealed variation in the composition of mt minichromosomes within the genus Hoplopleura . Hoplopleura sp. is the first species from the family Hoplopleuridae for which a complete fragmented mt genome has been sequenced. The new data provides useful genetic markers for studying the population genetics, molecular systematics and phylogenetics of blood-sucking lice.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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