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Original article
Lianyang Bai
Hunan Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1744-9448
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Benzobicyclon (BBC) is a β-triketone herbicide (bTH) used in rice paddy fields. It has the advantages of high efficiency, low toxicity, high crop safety, and good environmental compatibility, and shows efficacy against paddy weeds resistant to other types of herbicides. However, because BBC is not safe for some important indica rice varieties, it is currently only registered and applied in japonica rice areas.
Results
In this study, by analyzing haplotypes of the bTHs broad-spectrum resistance gene HIS1 and phenotypes for BBC in 493 major indica rice accessions in China, we identified a novel non-functional allelic variant of HIS1 besides the previously reported 28-bp deletion. Through detection with markers specific to the two non-functional mutations, it was clear that 25.4% of indica conventional varieties, 59.9% of restorers and 15.9% of sterile lines were susceptible to BBC. In addition, due to natural allelic variations of the HIS1 gene in sterile and restorer lines, some two-line hybrid sterile lines were sensitive to bTHs, and the corresponding restorers were resistant. We showed the potential of effectively using bTHs to solve the problem of two-line hybrid rice seed purity due to self-crossing of sterile lines during hybrid rice seed production. Finally, allelic variations of the HIS1 gene may also play an important role in the mechanized seed production of hybrid rice.
Conclusions
Our findings provide guidance for the application of BBC in indica rice areas, and provide a non-transgenic approach to solve the problem of seed purity of two-line hybrid rice.
Keywords
indica rice, hybrid rice, HIS1 gene, β-triketone herbicides, benzobicyclon
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On 23 Nov, 2020
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On 23 Nov, 2020
Editor invited
On 23 Nov, 2020
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Editorial decision: Minor revision
On 16 Nov, 2020
Review #2 received
Received 15 Nov, 2020
Review #1 received
Received 30 Oct, 2020
Reviewer #2 agreed
On 24 Oct, 2020
Reviewer #1 agreed
On 24 Oct, 2020
Reviewers invited
Invitations sent on 23 Oct, 2020
Editor assigned
On 19 Oct, 2020
Submission checks complete
On 18 Oct, 2020
Editor invited
On 18 Oct, 2020
Version 1
Posted 16 Jul, 2020
No comments provided
Editorial decision: Major revision
On 18 Aug, 2020
Review #2 received
Received 16 Aug, 2020
Review #1 received
Received 12 Aug, 2020
Reviewer #2 agreed
On 29 Jul, 2020
Reviewers invited
Invitations sent on 19 Jul, 2020
Reviewer #1 agreed
On 19 Jul, 2020
Editor assigned
On 15 Jul, 2020
First submitted
On 14 Jul, 2020
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On 14 Jul, 2020
Editor invited
On 14 Jul, 2020
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See the published version of this preprint at Rice.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Original article
Lianyang Bai
Hunan Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1744-9448
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Rice.
No community comments so far
Editorial decision: Accept
On 10 Dec, 2020
Editor assigned
On 09 Dec, 2020
Submission checks complete
On 09 Dec, 2020
Editor invited
On 09 Dec, 2020
No comments provided
Editor assigned
On 23 Nov, 2020
Submission checks complete
On 23 Nov, 2020
Editor invited
On 23 Nov, 2020
No comments provided
Editorial decision: Minor revision
On 16 Nov, 2020
Review #2 received
Received 15 Nov, 2020
Review #1 received
Received 30 Oct, 2020
Reviewer #2 agreed
On 24 Oct, 2020
Reviewer #1 agreed
On 24 Oct, 2020
Reviewers invited
Invitations sent on 23 Oct, 2020
Editor assigned
On 19 Oct, 2020
Submission checks complete
On 18 Oct, 2020
Editor invited
On 18 Oct, 2020
Version 1
Posted 16 Jul, 2020
No comments provided
Editorial decision: Major revision
On 18 Aug, 2020
Review #2 received
Received 16 Aug, 2020
Review #1 received
Received 12 Aug, 2020
Reviewer #2 agreed
On 29 Jul, 2020
Reviewers invited
Invitations sent on 19 Jul, 2020
Reviewer #1 agreed
On 19 Jul, 2020
Editor assigned
On 15 Jul, 2020
First submitted
On 14 Jul, 2020
Submission checks complete
On 14 Jul, 2020
Editor invited
On 14 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Rice.
Background
Benzobicyclon (BBC) is a β-triketone herbicide (bTH) used in rice paddy fields. It has the advantages of high efficiency, low toxicity, high crop safety, and good environmental compatibility, and shows efficacy against paddy weeds resistant to other types of herbicides. However, because BBC is not safe for some important indica rice varieties, it is currently only registered and applied in japonica rice areas.
Results
In this study, by analyzing haplotypes of the bTHs broad-spectrum resistance gene HIS1 and phenotypes for BBC in 493 major indica rice accessions in China, we identified a novel non-functional allelic variant of HIS1 besides the previously reported 28-bp deletion. Through detection with markers specific to the two non-functional mutations, it was clear that 25.4% of indica conventional varieties, 59.9% of restorers and 15.9% of sterile lines were susceptible to BBC. In addition, due to natural allelic variations of the HIS1 gene in sterile and restorer lines, some two-line hybrid sterile lines were sensitive to bTHs, and the corresponding restorers were resistant. We showed the potential of effectively using bTHs to solve the problem of two-line hybrid rice seed purity due to self-crossing of sterile lines during hybrid rice seed production. Finally, allelic variations of the HIS1 gene may also play an important role in the mechanized seed production of hybrid rice.
Conclusions
Our findings provide guidance for the application of BBC in indica rice areas, and provide a non-transgenic approach to solve the problem of seed purity of two-line hybrid rice.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
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