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Research
Xinghua Pan
Kunming Key Laboratory of Stem Cell and Regenerative Medicine
Corresponding Author
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Background: Age-related degeneration of lung tissues increases the risk of lung injury and exacerbates lung disease. It is also the main risk factor for chronic lung diseases (such as COPD, idiopathic pulmonary fibrosis, cancer, etc.).
Methods: we performed systematic screening, evaluation of elderly macaque model. A senile multiple organ dysfunction model was used to explored whether BMMSCS could improve degeneration of lung tissues in an elderly macaque model.
Results: Using model evaluation tests, we found that the average alveolar area, Mean linear intercept（MLI） and fibrosis area in the elderly macaque models were significantly larger than in young rhesus monkeys (P <0.05), and the capillary density around the alveoli was significantly lower than in young macaque models (P <0.05). Intraveous infusion of BMMSCS reduced the degree of pulmonary fibrosis in elderly macaque, increased the density of capillaries around the alveoli (P <0.05), and the number of type Ⅱ alveolar epithelium in elderly macaque (P <0.05). BMMSCS infusion reduced lung tissue ROS level, systemic and lung tissue inflammation level and Treg cell ratio in elderly macaque model（P＜0.05）. Indirect co-cultivation revealed that BMMSCS reduced the expression of senescence-related genes, ROS levels, apoptosis rate of aging type Ⅱ alveolar epithelial cells (A549 cells) and promoted their proliferation (P＜0.05).
Conclusions: BMMSC treatment can improve age-related degeneration of macaque lung tissue
Keywords
BMMSCS, lung degeneration, type Ⅱ alveolar epithelial cells, macaque
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Peer Review Timeline
CURRENT STATUS: Published
View the published article at Stem Cell Research & Therapy.
No community comments so far
Editorial decision: Accept
On 27 Jan, 2021
Reviewer #1 agreed
On 25 Jan, 2021
Review #1 received
Received 25 Jan, 2021
Reviewers invited
Invitations sent on 16 Jan, 2021
Editor assigned
On 14 Jan, 2021
Submission checks complete
On 14 Jan, 2021
Editor invited
On 14 Jan, 2021
No comments provided
Editorial decision: Minor Revision
On 09 Jan, 2021
Review #2 received
Received 06 Jan, 2021
Reviewer #2 agreed
On 20 Dec, 2020
Review #1 received
Received 20 Dec, 2020
Reviewer #1 agreed
On 20 Dec, 2020
Reviewers invited
Invitations sent on 16 Dec, 2020
Editor assigned
On 13 Dec, 2020
Submission checks complete
On 13 Dec, 2020
Editor invited
On 13 Dec, 2020
Version 2
Posted 03 Sep, 2020
No comments provided
Editorial decision: Major Revision
On 17 Sep, 2020
Review #1 received
Received 15 Sep, 2020
Reviewer #2 agreed
On 09 Sep, 2020
Review #2 received
Received 09 Sep, 2020
Reviewer #1 agreed
On 08 Sep, 2020
Reviewers invited
Invitations sent on 05 Sep, 2020
Editor assigned
On 02 Sep, 2020
Submission checks complete
On 01 Sep, 2020
Editor invited
On 01 Sep, 2020
No comments provided
Review #1 received
Received 25 Jul, 2020
Editorial decision: Major Revision
On 25 Jul, 2020
Review #5 received
Received 24 Jul, 2020
Review #2 received
Received 24 Jul, 2020
Reviewer #5 agreed
On 23 Jul, 2020
Reviewer #4 agreed
On 22 Jul, 2020
Reviewer #2 agreed
On 20 Jul, 2020
Reviewer #3 agreed
On 20 Jul, 2020
Reviewers invited
Invitations sent on 19 Jul, 2020
Reviewer #1 agreed
On 19 Jul, 2020
Editor assigned
On 16 Jul, 2020
Editor invited
On 15 Jul, 2020
Submission checks complete
On 15 Jul, 2020
First submitted
On 13 Jul, 2020
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Subject Areas
Stem Cell & Developmental Cell Biology
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Stem Cell Research & Therapy.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Xinghua Pan
Kunming Key Laboratory of Stem Cell and Regenerative Medicine
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Stem Cell Research & Therapy.
No community comments so far
Editorial decision: Accept
On 27 Jan, 2021
Reviewer #1 agreed
On 25 Jan, 2021
Review #1 received
Received 25 Jan, 2021
Reviewers invited
Invitations sent on 16 Jan, 2021
Editor assigned
On 14 Jan, 2021
Submission checks complete
On 14 Jan, 2021
Editor invited
On 14 Jan, 2021
No comments provided
Editorial decision: Minor Revision
On 09 Jan, 2021
Review #2 received
Received 06 Jan, 2021
Reviewer #2 agreed
On 20 Dec, 2020
Review #1 received
Received 20 Dec, 2020
Reviewer #1 agreed
On 20 Dec, 2020
Reviewers invited
Invitations sent on 16 Dec, 2020
Editor assigned
On 13 Dec, 2020
Submission checks complete
On 13 Dec, 2020
Editor invited
On 13 Dec, 2020
Version 2
Posted 03 Sep, 2020
No comments provided
Editorial decision: Major Revision
On 17 Sep, 2020
Review #1 received
Received 15 Sep, 2020
Reviewer #2 agreed
On 09 Sep, 2020
Review #2 received
Received 09 Sep, 2020
Reviewer #1 agreed
On 08 Sep, 2020
Reviewers invited
Invitations sent on 05 Sep, 2020
Editor assigned
On 02 Sep, 2020
Submission checks complete
On 01 Sep, 2020
Editor invited
On 01 Sep, 2020
No comments provided
Review #1 received
Received 25 Jul, 2020
Editorial decision: Major Revision
On 25 Jul, 2020
Review #5 received
Received 24 Jul, 2020
Review #2 received
Received 24 Jul, 2020
Reviewer #5 agreed
On 23 Jul, 2020
Reviewer #4 agreed
On 22 Jul, 2020
Reviewer #2 agreed
On 20 Jul, 2020
Reviewer #3 agreed
On 20 Jul, 2020
Reviewers invited
Invitations sent on 19 Jul, 2020
Reviewer #1 agreed
On 19 Jul, 2020
Editor assigned
On 16 Jul, 2020
Editor invited
On 15 Jul, 2020
Submission checks complete
On 15 Jul, 2020
First submitted
On 13 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Stem Cell & Developmental Cell Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Stem Cell Research & Therapy.
Background: Age-related degeneration of lung tissues increases the risk of lung injury and exacerbates lung disease. It is also the main risk factor for chronic lung diseases (such as COPD, idiopathic pulmonary fibrosis, cancer, etc.).
Methods: we performed systematic screening, evaluation of elderly macaque model. A senile multiple organ dysfunction model was used to explored whether BMMSCS could improve degeneration of lung tissues in an elderly macaque model.
Results: Using model evaluation tests, we found that the average alveolar area, Mean linear intercept（MLI） and fibrosis area in the elderly macaque models were significantly larger than in young rhesus monkeys (P <0.05), and the capillary density around the alveoli was significantly lower than in young macaque models (P <0.05). Intraveous infusion of BMMSCS reduced the degree of pulmonary fibrosis in elderly macaque, increased the density of capillaries around the alveoli (P <0.05), and the number of type Ⅱ alveolar epithelium in elderly macaque (P <0.05). BMMSCS infusion reduced lung tissue ROS level, systemic and lung tissue inflammation level and Treg cell ratio in elderly macaque model（P＜0.05）. Indirect co-cultivation revealed that BMMSCS reduced the expression of senescence-related genes, ROS levels, apoptosis rate of aging type Ⅱ alveolar epithelial cells (A549 cells) and promoted their proliferation (P＜0.05).
Conclusions: BMMSC treatment can improve age-related degeneration of macaque lung tissue
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