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Research note
Iloduba Nnaemeka Aghanya
Nnamdi Azikiwe University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4481-4834
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective The acquisition of carbapenemase-producing organisms in healthcare settings is a major threat and has serious implications for public health. Previous reports regarding carbapenemase-producing Enterobacteriaceae from fomites are limited. This study aimed at analysing the antimicrobial resistance patterns, and prevalence of carbapenemase-producing Klebsiella pneumoniae in the ward environments of a tertiary health institution in Nigeria.
Results A total of 142 bacteria were isolated from 534 fomites in the hospital wards, and of the 142 isolates, 15(10.6%) were confirmed to be Klebsiella pneumoniae . The prevalence of Klebsiella pneumoniae in all the 534 samples was 15/534(2.8%), while the prevalence of carbapenemase-producing Klebsiella pneumoniae was 8/534(1.5%). Multi-drug resistance was detected in 15/15(100%) of the Klebsiella pneumoniae isolated. Although no Klebsiella pneumoniae Carbapenemase ( bla KPC ) gene was expressed in any of these isolates, 8/15(53.3%) of these isolates were confirmed positive for carbapenemase production using the Modified Hodge Test. The commonest sites that harboured carbapenem-resistant Klebsiella pneumoniae were the beds 6/15(40%). Maximum resistance (100%) was observed against ampicillin, trimethoprim-sulfamethoxazole, cefuroxime, and tetracycline. In conclusion, the prevalence of carbapenemase-producing Klebsiella pneumoniae fomite colonization in the NAUTH ward environment is low, thus buttressing the need to reinforce strict infection control policies the hospital.
Keywords
Multidrug-resistant, Klebsiella pneumoniae, Carbapenemase, Oxacillinase, Fomites
Figure 1
This is a list of supplementary files associated with this preprint. Click to download.
- TableS1.docx
Table S1. Quantification of Bacterial DNA present in the carbapenemase producing Klebsiella pneumoniae using NanoDrop® ND-1000
- TableS2.docx
Table S2. Primer Sequences used in the Study
- TableS3.docx
Table S3. Distribution of Organisms Isolated from the Sampled Hospital Sources
- TableS4.docx
Table S4 Distribution of Klebsiella pneumoniae in the sampled environmental sources in NAUTH
- TableS5.xlsx
Table S5. Antibiogram and carbapenemase production profile of the Klebsiella pneumoniae isolates obtained from the sampled sources.
- FigureS1.docx
Figure S1. Modified Hodge Test showing clover leaf appearance.
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research note
Iloduba Nnaemeka Aghanya
Nnamdi Azikiwe University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4481-4834
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
The most recent version of this article is available here.
No community comments so far
Version 2
Posted 11 Sep, 2020
No comments provided
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Hospital Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The most recent version of this article is available here.
Objective The acquisition of carbapenemase-producing organisms in healthcare settings is a major threat and has serious implications for public health. Previous reports regarding carbapenemase-producing Enterobacteriaceae from fomites are limited. This study aimed at analysing the antimicrobial resistance patterns, and prevalence of carbapenemase-producing Klebsiella pneumoniae in the ward environments of a tertiary health institution in Nigeria.
Results A total of 142 bacteria were isolated from 534 fomites in the hospital wards, and of the 142 isolates, 15(10.6%) were confirmed to be Klebsiella pneumoniae . The prevalence of Klebsiella pneumoniae in all the 534 samples was 15/534(2.8%), while the prevalence of carbapenemase-producing Klebsiella pneumoniae was 8/534(1.5%). Multi-drug resistance was detected in 15/15(100%) of the Klebsiella pneumoniae isolated. Although no Klebsiella pneumoniae Carbapenemase ( bla KPC ) gene was expressed in any of these isolates, 8/15(53.3%) of these isolates were confirmed positive for carbapenemase production using the Modified Hodge Test. The commonest sites that harboured carbapenem-resistant Klebsiella pneumoniae were the beds 6/15(40%). Maximum resistance (100%) was observed against ampicillin, trimethoprim-sulfamethoxazole, cefuroxime, and tetracycline. In conclusion, the prevalence of carbapenemase-producing Klebsiella pneumoniae fomite colonization in the NAUTH ward environment is low, thus buttressing the need to reinforce strict infection control policies the hospital.
Figure 1
This is a list of supplementary files associated with this preprint. Click to download.
- TableS1.docx
Table S1. Quantification of Bacterial DNA present in the carbapenemase producing Klebsiella pneumoniae using NanoDrop® ND-1000
- TableS2.docx
Table S2. Primer Sequences used in the Study
- TableS3.docx
Table S3. Distribution of Organisms Isolated from the Sampled Hospital Sources
- TableS4.docx
Table S4 Distribution of Klebsiella pneumoniae in the sampled environmental sources in NAUTH
- TableS5.xlsx
Table S5. Antibiogram and carbapenemase production profile of the Klebsiella pneumoniae isolates obtained from the sampled sources.
- FigureS1.docx
Figure S1. Modified Hodge Test showing clover leaf appearance.
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