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Detection of Carbapenemase-producing Klebsiella pneumoniae isolated from Environmental Sources in a Tertiary Health Institution in Nigeria.
Iloduba Nnaemeka Aghanya
Nnamdi Azikiwe University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4481-4834
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective
The acquisition of carbapenemase-producing organisms in healthcare settings is a significant threat and has dire implications for public health. Previous reports regarding carbapenemase-producing Enterobacteriaceae from fomites are limited. This study aimed to analyse the antimicrobial resistance patterns and prevalence of carbapenemase-producing Klebsiella pneumoniae in the ward environments of a tertiary health institution in Nigeria.
Results
One hundred and forty-two bacteria were isolated from 534 fomites in the hospital wards, and out of these, 15(10.6%) were K. pneumoniae. Therefore, the prevalence of K. pneumoniae in all the samples was 15/534(2.8%), while that of carbapenemase-producing K. pneumoniae was 8/534(1.5%). Multi-drug resistance was detected in 15/15(100%) of the K. pneumoniae isolated. All the K. pneumoniae isolates were resistant to ampicillin, trimethoprim-sulfamethoxazole, cefuroxime, and tetracycline. Although 8/15(53.3%) of the isolates were confirmed positive for carbapenemase production using the modified Hodge test, no Klebsiella pneumoniae carbapenemase gene (blaKPC) was detected. The most frequent sites that harboured carbapenem-resistant K. pneumoniae were the beds 6/15(40%). Hence, the prevalence of carbapenemase-producing K. pneumoniae fomite colonisation in the NAUTH ward environment was low.
Keywords
Multidrug-resistant, Klebsiella pneumoniae, Carbapenemase, Oxacillinase, Fomites
Figure 1
This is a list of supplementary files associated with this preprint. Click to download.
- TableS1.docx
Additional file 1: Table S1. Quantification of Bacterial DNA present in the carbapenemase producing Klebsiella pneumoniae using NanoDrop® ND-1000
- TableS2.docx
Additional file 2: Table S2. Primer Sequences used in the study
- TableS3.docx
Additional file 2: Table S2. Primer Sequences used in the study
- TableS4.docx
Additional file 4: Table S4 Distribution of Klebsiella pneumoniae in the sampled environmental sources in NAUTH
- TableS5.xlsx
Additional file 5: Table S5. Antibiogram and carbapenemase production profile of the Klebsiella pneumoniae isolates obtained from the sampled sources.
- FigureS1.docx
Additional file 6: Figure S1. Modified Hodge test showing clover leaf appearance.
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On 01 Aug, 2020
Reviewer #2 agreed
On 01 Aug, 2020
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Subject Areas
Hospital Medicine
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This preprint is under consideration at BMC Research Notes. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research note
Detection of Carbapenemase-producing Klebsiella pneumoniae isolated from Environmental Sources in a Tertiary Health Institution in Nigeria.
Iloduba Nnaemeka Aghanya
Nnamdi Azikiwe University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4481-4834
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 3
Posted 04 Jan, 2021
No community comments so far
Editorial decision: Minor revision
On 20 Dec, 2020
Editor assigned
On 14 Dec, 2020
Submission checks complete
On 14 Dec, 2020
Editor invited
On 14 Dec, 2020
Version (no preprint)
Posted 08 Dec, 2020
Editor assigned
On 08 Dec, 2020
Submission checks complete
On 08 Dec, 2020
Editor invited
On 08 Dec, 2020
This version was not preprinted
No comments provided
Editorial decision: Major revision
On 11 Sep, 2020
Editor assigned
On 09 Sep, 2020
Submission checks complete
On 08 Sep, 2020
Editor invited
On 08 Sep, 2020
No comments provided
Editorial decision: Minor revision
On 21 Aug, 2020
Review #2 received
Received 19 Aug, 2020
Review #1 received
Received 19 Aug, 2020
Reviewer #1 agreed
On 01 Aug, 2020
Reviewer #2 agreed
On 01 Aug, 2020
Reviewers invited
Invitations sent on 29 Jul, 2020
Editor assigned
On 16 Jul, 2020
Submission checks complete
On 15 Jul, 2020
Editor invited
On 15 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Hospital Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Objective
The acquisition of carbapenemase-producing organisms in healthcare settings is a significant threat and has dire implications for public health. Previous reports regarding carbapenemase-producing Enterobacteriaceae from fomites are limited. This study aimed to analyse the antimicrobial resistance patterns and prevalence of carbapenemase-producing Klebsiella pneumoniae in the ward environments of a tertiary health institution in Nigeria.
Results
One hundred and forty-two bacteria were isolated from 534 fomites in the hospital wards, and out of these, 15(10.6%) were K. pneumoniae. Therefore, the prevalence of K. pneumoniae in all the samples was 15/534(2.8%), while that of carbapenemase-producing K. pneumoniae was 8/534(1.5%). Multi-drug resistance was detected in 15/15(100%) of the K. pneumoniae isolated. All the K. pneumoniae isolates were resistant to ampicillin, trimethoprim-sulfamethoxazole, cefuroxime, and tetracycline. Although 8/15(53.3%) of the isolates were confirmed positive for carbapenemase production using the modified Hodge test, no Klebsiella pneumoniae carbapenemase gene (blaKPC) was detected. The most frequent sites that harboured carbapenem-resistant K. pneumoniae were the beds 6/15(40%). Hence, the prevalence of carbapenemase-producing K. pneumoniae fomite colonisation in the NAUTH ward environment was low.
Figure 1