Detection of Carbapenemase-producing Klebsiella pneumoniae isolated from Environmental Sources in a Tertiary Health Institution in Nigeria.
Objective
The acquisition of carbapenemase-producing organisms in healthcare settings is a significant threat and has dire implications for public health. Previous reports regarding carbapenemase-producing Enterobacteriaceae from fomites are limited. This study aimed to analyse the antimicrobial resistance patterns and prevalence of carbapenemase-producing Klebsiella pneumoniae in the ward environments of a tertiary health institution in Nigeria.
Results
One hundred and forty-two bacteria were isolated from 534 fomites in the hospital wards, and out of these, 15(10.6%) were K. pneumoniae. Therefore, the prevalence of K. pneumoniae in all the samples was 15/534(2.8%), while that of carbapenemase-producing K. pneumoniae was 8/534(1.5%). Multi-drug resistance was detected in 15/15(100%) of the K. pneumoniae isolated. All the K. pneumoniae isolates were resistant to ampicillin, trimethoprim-sulfamethoxazole, cefuroxime, and tetracycline. Although 8/15(53.3%) of the isolates were confirmed positive for carbapenemase production using the modified Hodge test, no Klebsiella pneumoniae carbapenemase gene (blaKPC) was detected. The most frequent sites that harboured carbapenem-resistant K. pneumoniae were the beds 6/15(40%). Hence, the prevalence of carbapenemase-producing K. pneumoniae fomite colonisation in the NAUTH ward environment was low.
Figure 1
This is a list of supplementary files associated with this preprint. Click to download.
Additional file 1: Table S1. Quantification of Bacterial DNA present in the carbapenemase producing Klebsiella pneumoniae using NanoDrop® ND-1000
Additional file 2: Table S2. Primer Sequences used in the study
Additional file 2: Table S2. Primer Sequences used in the study
Additional file 4: Table S4 Distribution of Klebsiella pneumoniae in the sampled environmental sources in NAUTH
Additional file 5: Table S5. Antibiogram and carbapenemase production profile of the Klebsiella pneumoniae isolates obtained from the sampled sources.
Additional file 6: Figure S1. Modified Hodge test showing clover leaf appearance.
Posted 04 Jan, 2021
On 20 Dec, 2020
On 14 Dec, 2020
On 14 Dec, 2020
On 14 Dec, 2020
Posted 08 Dec, 2020
On 08 Dec, 2020
On 08 Dec, 2020
On 08 Dec, 2020
On 11 Sep, 2020
On 09 Sep, 2020
On 08 Sep, 2020
On 08 Sep, 2020
On 21 Aug, 2020
Received 19 Aug, 2020
Received 19 Aug, 2020
On 01 Aug, 2020
On 01 Aug, 2020
Invitations sent on 29 Jul, 2020
On 16 Jul, 2020
On 15 Jul, 2020
On 15 Jul, 2020
Detection of Carbapenemase-producing Klebsiella pneumoniae isolated from Environmental Sources in a Tertiary Health Institution in Nigeria.
Posted 04 Jan, 2021
On 20 Dec, 2020
On 14 Dec, 2020
On 14 Dec, 2020
On 14 Dec, 2020
Posted 08 Dec, 2020
On 08 Dec, 2020
On 08 Dec, 2020
On 08 Dec, 2020
On 11 Sep, 2020
On 09 Sep, 2020
On 08 Sep, 2020
On 08 Sep, 2020
On 21 Aug, 2020
Received 19 Aug, 2020
Received 19 Aug, 2020
On 01 Aug, 2020
On 01 Aug, 2020
Invitations sent on 29 Jul, 2020
On 16 Jul, 2020
On 15 Jul, 2020
On 15 Jul, 2020
Objective
The acquisition of carbapenemase-producing organisms in healthcare settings is a significant threat and has dire implications for public health. Previous reports regarding carbapenemase-producing Enterobacteriaceae from fomites are limited. This study aimed to analyse the antimicrobial resistance patterns and prevalence of carbapenemase-producing Klebsiella pneumoniae in the ward environments of a tertiary health institution in Nigeria.
Results
One hundred and forty-two bacteria were isolated from 534 fomites in the hospital wards, and out of these, 15(10.6%) were K. pneumoniae. Therefore, the prevalence of K. pneumoniae in all the samples was 15/534(2.8%), while that of carbapenemase-producing K. pneumoniae was 8/534(1.5%). Multi-drug resistance was detected in 15/15(100%) of the K. pneumoniae isolated. All the K. pneumoniae isolates were resistant to ampicillin, trimethoprim-sulfamethoxazole, cefuroxime, and tetracycline. Although 8/15(53.3%) of the isolates were confirmed positive for carbapenemase production using the modified Hodge test, no Klebsiella pneumoniae carbapenemase gene (blaKPC) was detected. The most frequent sites that harboured carbapenem-resistant K. pneumoniae were the beds 6/15(40%). Hence, the prevalence of carbapenemase-producing K. pneumoniae fomite colonisation in the NAUTH ward environment was low.
Figure 1