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Research Article
Danning Xu
Zhongkai University of Agriculture and Engineering
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
The Polysaccharide of Atractylodes macrocephala Koidz (PAMK) has been reported to play an important role in improving the immune function of mammals and poultry. But the molecular mechanism in cell is still not unclear. For investigating the mechanism in mice, RAW264.7 were cultured and divided into six groups (cell, NC, siTLR4, NC + PAMK, PAMK, siTLR4 + PAMK). The serum cytokines, mRNA relative expression level of cytokines and TLR4 signal pathway, the protein expression level of TLR4 signal pathway were measured. The results showed PAMK could increase the level of IL-4, IL-6, TNF-α, IFN-γ, and increase the mRNA relative expression level and protein expression level of TLR4, MyD88, IKK, NF-κB. In conclusion, PAMK may regulate the immue activity of RAW264.7 through the TLR4/MyD88/NF-κB pathway.
Keywords
PAMK, RAW264.7, TLR4/NF-κB pathway
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No competing interests reported.
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This is a preprint. It has not completed peer review.
Research Article
Danning Xu
Zhongkai University of Agriculture and Engineering
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 27 Apr, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Immunology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The Polysaccharide of Atractylodes macrocephala Koidz (PAMK) has been reported to play an important role in improving the immune function of mammals and poultry. But the molecular mechanism in cell is still not unclear. For investigating the mechanism in mice, RAW264.7 were cultured and divided into six groups (cell, NC, siTLR4, NC + PAMK, PAMK, siTLR4 + PAMK). The serum cytokines, mRNA relative expression level of cytokines and TLR4 signal pathway, the protein expression level of TLR4 signal pathway were measured. The results showed PAMK could increase the level of IL-4, IL-6, TNF-α, IFN-γ, and increase the mRNA relative expression level and protein expression level of TLR4, MyD88, IKK, NF-κB. In conclusion, PAMK may regulate the immue activity of RAW264.7 through the TLR4/MyD88/NF-κB pathway.
Figure 1
Figure 2
Figure 3
Figure 4
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