Identification of Dissociation Factors in Pancreatic Cancer using a Mass Spectrometry-Based Proteomic Approach
Backgroud Pancreatic cancer is a highly malignant tumor of the digestive system. This secretome of pancreatic cancer is key to its progression and metastasis. But different methods of protein extraction affect the final results. In other words, the real secretion of proteins in cancer cells has been changed. Based on mass spectrometry, we analyze the secretome from the serum-containing and serum-free medium, using different protein pretreatment methods. This study aims to identify dissociation factors in pancreatic cancer. Methods In this study, pancreatic cancer cells were cultured in serum-containing or serum-free medium, and the corresponding supernatants were extracted as samples. Subsequently, the above samples were separated by size exclusion chromatography (SEC), and peptide segments were identified by LC-MS/MS. The final results were identified via the hamster secreted protein database and a public database. Results Although the number of identified proteins in the serum-free medium group was high, the real secretion of proteins in pancreatic cancer cells was changed. There were six significant secreted proteins in the serum-containing medium group. Survival analysis via the TCGA database suggested that patients with higher expression levels of YWHAG showed a worse overall survival rate than those with lower YWHAG expression. Conclusions Our study demonstrated the results in the serum-containing medium group were more similar to the real secretome of pancreatic cancer cells. YWHAG could be used as a prognostic indicator for pancreatic cancer.
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Posted 07 Jan, 2020
On 20 Jan, 2020
On 07 Jan, 2020
On 03 Jan, 2020
On 02 Jan, 2020
On 02 Jan, 2020
On 30 Dec, 2019
Received 25 Dec, 2019
On 16 Dec, 2019
Invitations sent on 14 Dec, 2019
On 09 Dec, 2019
On 08 Dec, 2019
On 08 Dec, 2019
Received 20 Oct, 2019
On 20 Oct, 2019
On 07 Oct, 2019
Received 27 Sep, 2019
On 12 Sep, 2019
Invitations sent on 02 Sep, 2019
On 29 Aug, 2019
On 22 Aug, 2019
On 22 Aug, 2019
On 13 Aug, 2019
Identification of Dissociation Factors in Pancreatic Cancer using a Mass Spectrometry-Based Proteomic Approach
Posted 07 Jan, 2020
On 20 Jan, 2020
On 07 Jan, 2020
On 03 Jan, 2020
On 02 Jan, 2020
On 02 Jan, 2020
On 30 Dec, 2019
Received 25 Dec, 2019
On 16 Dec, 2019
Invitations sent on 14 Dec, 2019
On 09 Dec, 2019
On 08 Dec, 2019
On 08 Dec, 2019
Received 20 Oct, 2019
On 20 Oct, 2019
On 07 Oct, 2019
Received 27 Sep, 2019
On 12 Sep, 2019
Invitations sent on 02 Sep, 2019
On 29 Aug, 2019
On 22 Aug, 2019
On 22 Aug, 2019
On 13 Aug, 2019
Backgroud Pancreatic cancer is a highly malignant tumor of the digestive system. This secretome of pancreatic cancer is key to its progression and metastasis. But different methods of protein extraction affect the final results. In other words, the real secretion of proteins in cancer cells has been changed. Based on mass spectrometry, we analyze the secretome from the serum-containing and serum-free medium, using different protein pretreatment methods. This study aims to identify dissociation factors in pancreatic cancer. Methods In this study, pancreatic cancer cells were cultured in serum-containing or serum-free medium, and the corresponding supernatants were extracted as samples. Subsequently, the above samples were separated by size exclusion chromatography (SEC), and peptide segments were identified by LC-MS/MS. The final results were identified via the hamster secreted protein database and a public database. Results Although the number of identified proteins in the serum-free medium group was high, the real secretion of proteins in pancreatic cancer cells was changed. There were six significant secreted proteins in the serum-containing medium group. Survival analysis via the TCGA database suggested that patients with higher expression levels of YWHAG showed a worse overall survival rate than those with lower YWHAG expression. Conclusions Our study demonstrated the results in the serum-containing medium group were more similar to the real secretome of pancreatic cancer cells. YWHAG could be used as a prognostic indicator for pancreatic cancer.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10