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Research article
Xiaodong Tan
Shengjing Hospital of China Medical University
Corresponding Author
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Backgroud Pancreatic cancer is a highly malignant tumor of the digestive system. This secretome of pancreatic cancer is key to its progression and metastasis. But different methods of protein extraction affect the final results. In other words, the real secretion of proteins in cancer cells has been changed. Based on mass spectrometry, we analyze the secretome from the serum-containing and serum-free medium, using different protein pretreatment methods. This study aims to identify dissociation factors in pancreatic cancer. Methods In this study, pancreatic cancer cells were cultured in serum-containing or serum-free medium, and the corresponding supernatants were extracted as samples. Subsequently, the above samples were separated by size exclusion chromatography (SEC), and peptide segments were identified by LC-MS/MS. The final results were identified via the hamster secreted protein database and a public database. Results Although the number of identified proteins in the serum-free medium group was high, the real secretion of proteins in pancreatic cancer cells was changed. There were six significant secreted proteins in the serum-containing medium group. Survival analysis via the TCGA database suggested that patients with higher expression levels of YWHAG showed a worse overall survival rate than those with lower YWHAG expression. Conclusions Our study demonstrated the results in the serum-containing medium group were more similar to the real secretome of pancreatic cancer cells. YWHAG could be used as a prognostic indicator for pancreatic cancer.
Keywords
pancreatic cancer, secreted proteome, SEC, mass spectrum
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CURRENT STATUS: Published
View the published article at BMC Cancer.
Version 3
Posted 07 Jan, 2020
No community comments so far
Editorial decision: Accept
On 07 Jan, 2020
Editor assigned
On 03 Jan, 2020
Submission checks complete
On 02 Jan, 2020
Editor invited
On 02 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 30 Dec, 2019
Review #1 received
Received 25 Dec, 2019
Reviewer #1 agreed
On 16 Dec, 2019
Reviewers invited
Invitations sent on 14 Dec, 2019
Editor assigned
On 09 Dec, 2019
Submission checks complete
On 08 Dec, 2019
Editor invited
On 08 Dec, 2019
No comments provided
Review #2 received
Received 20 Oct, 2019
Editorial decision: Major revision
On 20 Oct, 2019
Reviewer #2 agreed
On 07 Oct, 2019
Review #1 received
Received 27 Sep, 2019
Reviewer #1 agreed
On 12 Sep, 2019
Reviewers invited
Invitations sent on 02 Sep, 2019
Editor assigned
On 29 Aug, 2019
Submission checks complete
On 22 Aug, 2019
Editor invited
On 22 Aug, 2019
First submitted
On 13 Aug, 2019
Metrics
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Subject Areas
Cancer Biology
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See the published version of this preprint at BMC Cancer.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Xiaodong Tan
Shengjing Hospital of China Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Cancer.
Version 3
Posted 07 Jan, 2020
No community comments so far
Editorial decision: Accept
On 07 Jan, 2020
Editor assigned
On 03 Jan, 2020
Submission checks complete
On 02 Jan, 2020
Editor invited
On 02 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 30 Dec, 2019
Review #1 received
Received 25 Dec, 2019
Reviewer #1 agreed
On 16 Dec, 2019
Reviewers invited
Invitations sent on 14 Dec, 2019
Editor assigned
On 09 Dec, 2019
Submission checks complete
On 08 Dec, 2019
Editor invited
On 08 Dec, 2019
No comments provided
Review #2 received
Received 20 Oct, 2019
Editorial decision: Major revision
On 20 Oct, 2019
Reviewer #2 agreed
On 07 Oct, 2019
Review #1 received
Received 27 Sep, 2019
Reviewer #1 agreed
On 12 Sep, 2019
Reviewers invited
Invitations sent on 02 Sep, 2019
Editor assigned
On 29 Aug, 2019
Submission checks complete
On 22 Aug, 2019
Editor invited
On 22 Aug, 2019
First submitted
On 13 Aug, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cancer Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Cancer.
Backgroud Pancreatic cancer is a highly malignant tumor of the digestive system. This secretome of pancreatic cancer is key to its progression and metastasis. But different methods of protein extraction affect the final results. In other words, the real secretion of proteins in cancer cells has been changed. Based on mass spectrometry, we analyze the secretome from the serum-containing and serum-free medium, using different protein pretreatment methods. This study aims to identify dissociation factors in pancreatic cancer. Methods In this study, pancreatic cancer cells were cultured in serum-containing or serum-free medium, and the corresponding supernatants were extracted as samples. Subsequently, the above samples were separated by size exclusion chromatography (SEC), and peptide segments were identified by LC-MS/MS. The final results were identified via the hamster secreted protein database and a public database. Results Although the number of identified proteins in the serum-free medium group was high, the real secretion of proteins in pancreatic cancer cells was changed. There were six significant secreted proteins in the serum-containing medium group. Survival analysis via the TCGA database suggested that patients with higher expression levels of YWHAG showed a worse overall survival rate than those with lower YWHAG expression. Conclusions Our study demonstrated the results in the serum-containing medium group were more similar to the real secretome of pancreatic cancer cells. YWHAG could be used as a prognostic indicator for pancreatic cancer.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
This is a list of supplementary files associated with this preprint. Click to download.
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