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Research Article
Kinga Maria Pilarska
Wrocław University of Environmental and Life Sciences: Uniwersytet Przyrodniczy we Wroclawiu
Corresponding Author
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Carnivorous plant of Sarracenia genus are used by human in folk medicine for centuries. The reason for this phenomenon is biochemical composition of Sarracenia plants, which possess many bioactive compounds with anti-inflammatory, antioxidant, antiviral and antibacterial properties.
The subject of this research was genetic transformation of Sarracenia purpurea L. with Agrobacterium rhizogenes strain 15834, 9402 and A4 by using two alternatively methods of bacterial injection or co-culture the bacteria with plants explants. These studies confirmed the possibility of hairy roots induction in S. purpurea using the strain of A. rhizogenes 15834 and the injection method. Seven lines of transformed plants, exhibiting the integration of rolB gene, were obtained. The formed hairy roots showed morphological differences in comparison to the roots of unmodified plants. A mathematical model was used to optimize the conditions for the extraction of bioactive compounds. Extracts isolated under optimal conditions from transformed plants showed biochemical changes i.e., the increase in the accumulation of total polyphenols and triterpenes in comparison to untransformed plants, especially when induced roots were analyzed. HPLC analysis showed increase in the level of betulinic acid in some transformed Sarracenia lines. Betulinic acid remains pentacyclic triterpenoid compound with high pharmacological significance. The further work connected with isolation and identification of the other bioactive compounds will be done in the nearest future.
Keywords
hairy roots, Agrobacterium rhizogenes, carnivorous plants, Sarracenia purpurea L. composite plants
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Kinga Maria Pilarska
Wrocław University of Environmental and Life Sciences: Uniwersytet Przyrodniczy we Wroclawiu
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 03 May, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Horticulture
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Carnivorous plant of Sarracenia genus are used by human in folk medicine for centuries. The reason for this phenomenon is biochemical composition of Sarracenia plants, which possess many bioactive compounds with anti-inflammatory, antioxidant, antiviral and antibacterial properties.
The subject of this research was genetic transformation of Sarracenia purpurea L. with Agrobacterium rhizogenes strain 15834, 9402 and A4 by using two alternatively methods of bacterial injection or co-culture the bacteria with plants explants. These studies confirmed the possibility of hairy roots induction in S. purpurea using the strain of A. rhizogenes 15834 and the injection method. Seven lines of transformed plants, exhibiting the integration of rolB gene, were obtained. The formed hairy roots showed morphological differences in comparison to the roots of unmodified plants. A mathematical model was used to optimize the conditions for the extraction of bioactive compounds. Extracts isolated under optimal conditions from transformed plants showed biochemical changes i.e., the increase in the accumulation of total polyphenols and triterpenes in comparison to untransformed plants, especially when induced roots were analyzed. HPLC analysis showed increase in the level of betulinic acid in some transformed Sarracenia lines. Betulinic acid remains pentacyclic triterpenoid compound with high pharmacological significance. The further work connected with isolation and identification of the other bioactive compounds will be done in the nearest future.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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