Analysis Of Candidate Genes Expected To Be Essential For Melanoma Surviving
Background
New approach for treating cancer is to influence on vital genes for tumors. A number of attempts have led to discovery candidate genes that are mean to be essential for surviving of tumor cells of different types. In this work, we tried to analyze genes that were previously found to be essential for melanoma surviving. We performed transient siRNA-mediated knockdown of UNC45A, STK11IP, RHPN2 and ZNFX1 in melanoma cell line A375. After that, we checked cell viability, proliferation and migrating rate in vitro.
Methods
For knockdown of the genes we used siRNA mediated interference. To deliver siRNA in cells we used transfection by Metafectene PRO. Cell viability and proliferation assessed by MTT assay. Cell migration was assessed by wound healing assay. Knockdown of genes was evaluated via RT-qPCR analysis.
Results
Our results indicate that knockdown of the genes leads to no statistically significant changes in cell survival according to viability assay. However, mobility assay shows that knockdown of each of the target genes accelerates the speed of cells migrating.
Conclusion
Our results do not confirm the hypothesis. Possible explaining of such controversial results could be in insufficient bioinformatical analysis or in need of multiplex knockdown of the genes.
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On 07 Oct, 2020
On 19 Sep, 2020
Received 19 Sep, 2020
On 16 Sep, 2020
Invitations sent on 16 Sep, 2020
On 16 Sep, 2020
On 15 Sep, 2020
On 15 Sep, 2020
Posted 17 Jul, 2020
On 19 Aug, 2020
Received 16 Aug, 2020
On 03 Aug, 2020
On 02 Aug, 2020
Invitations sent on 30 Jul, 2020
On 30 Jul, 2020
Received 30 Jul, 2020
On 20 Jul, 2020
On 19 Jul, 2020
On 15 Jul, 2020
On 14 Jul, 2020
Analysis Of Candidate Genes Expected To Be Essential For Melanoma Surviving
On 07 Oct, 2020
On 19 Sep, 2020
Received 19 Sep, 2020
On 16 Sep, 2020
Invitations sent on 16 Sep, 2020
On 16 Sep, 2020
On 15 Sep, 2020
On 15 Sep, 2020
Posted 17 Jul, 2020
On 19 Aug, 2020
Received 16 Aug, 2020
On 03 Aug, 2020
On 02 Aug, 2020
Invitations sent on 30 Jul, 2020
On 30 Jul, 2020
Received 30 Jul, 2020
On 20 Jul, 2020
On 19 Jul, 2020
On 15 Jul, 2020
On 14 Jul, 2020
Background
New approach for treating cancer is to influence on vital genes for tumors. A number of attempts have led to discovery candidate genes that are mean to be essential for surviving of tumor cells of different types. In this work, we tried to analyze genes that were previously found to be essential for melanoma surviving. We performed transient siRNA-mediated knockdown of UNC45A, STK11IP, RHPN2 and ZNFX1 in melanoma cell line A375. After that, we checked cell viability, proliferation and migrating rate in vitro.
Methods
For knockdown of the genes we used siRNA mediated interference. To deliver siRNA in cells we used transfection by Metafectene PRO. Cell viability and proliferation assessed by MTT assay. Cell migration was assessed by wound healing assay. Knockdown of genes was evaluated via RT-qPCR analysis.
Results
Our results indicate that knockdown of the genes leads to no statistically significant changes in cell survival according to viability assay. However, mobility assay shows that knockdown of each of the target genes accelerates the speed of cells migrating.
Conclusion
Our results do not confirm the hypothesis. Possible explaining of such controversial results could be in insufficient bioinformatical analysis or in need of multiplex knockdown of the genes.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7