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Research Article
Chitti Thawai
King Monkut's Institute of Tecnology Ladkrabang
Corresponding Author
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An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110T, was isolated from the surface-sterilised root of Coffea arabica L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110T is a member of the genus Streptomyces and showed the closest similarities to Streptomyces rapamycinicus NRRL B-5491T (99.1%), followed by S. iranensis HM 35T (99.0%). Strain CA3R110T contained LL-diaminopimelic acid in cell peptidoglycan, MK-9(H6), and MK-9(H8) as major menaquinone, iso-C16:0, iso-C15:0, C16:0 as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus Streptomyces. A low digital DNA–DNA hybridization (< 55.6%) and average nucleotide identity-blast (ANIb) (< 92.2%) values revealed that strain CA3R110T could be distinguished from any known Streptomyces species. With the differences in phenotypic and genotypic data, strain CA3R110T represents a novel species of genus Streptomyces, for which the name Streptomyces endocoffea sp. nov. is proposed. The type strain is CA3R110T (= TBRC 11245T = NBRC 114296T).
Keywords
Streptomyces, endophytic actinobacterium, 16S rRNA gene, Coffea arabica L.
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CURRENT STATUS: Under Review
Version 1
Posted 03 May, 2021
No community comments so far
Editorial decision: Major revisions
On 23 May, 2021
Reviews received
Received 01 May, 2021
Reviewers invited
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On 16 Apr, 2021
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On 16 Apr, 2021
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This preprint is under consideration at Antonie van Leeuwenhoek. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Chitti Thawai
King Monkut's Institute of Tecnology Ladkrabang
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 03 May, 2021
No community comments so far
Editorial decision: Major revisions
On 23 May, 2021
Reviews received
Received 01 May, 2021
Reviewers invited
Invitations sent on 28 Apr, 2021
Editor assigned
On 16 Apr, 2021
First submitted
On 16 Apr, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110T, was isolated from the surface-sterilised root of Coffea arabica L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110T is a member of the genus Streptomyces and showed the closest similarities to Streptomyces rapamycinicus NRRL B-5491T (99.1%), followed by S. iranensis HM 35T (99.0%). Strain CA3R110T contained LL-diaminopimelic acid in cell peptidoglycan, MK-9(H6), and MK-9(H8) as major menaquinone, iso-C16:0, iso-C15:0, C16:0 as major fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside were detected in the cell. The chemotaxonomic characteristics possessed the typical properties of the genus Streptomyces. A low digital DNA–DNA hybridization (< 55.6%) and average nucleotide identity-blast (ANIb) (< 92.2%) values revealed that strain CA3R110T could be distinguished from any known Streptomyces species. With the differences in phenotypic and genotypic data, strain CA3R110T represents a novel species of genus Streptomyces, for which the name Streptomyces endocoffea sp. nov. is proposed. The type strain is CA3R110T (= TBRC 11245T = NBRC 114296T).
Figure 1
This is a list of supplementary files associated with this preprint. Click to download.
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