Synergic Effect of Combined Therapy of Hyperbaric Oxygen and Adipose-derived Mesenchymal Stem Cells on Improving Neurological Function After Acute Traumatic Spinal Cord Injury in Rat

Background: This study tested whether combined hyperbaric oxygen (HBO) and allogenic adipose-derived mesenchymal stem cells (ADMSCs) would be superior to either one for improving the neurological function in rat after acute traumatic spinal cord injury (TSCI) in rat. Methods and Results: Adult-male SD rats (n=40) were equally categorized into group 1 (sham-operated control), group 2 (TSCI), group 3 (TSCI + HBO for 1.5h/day for 14 consecutive days after TSCI), group 4 (TSCI + ADMSCs/1.2x10 6 cells by intravenous injection at 3h and days 1/2 after TSCI) and group 5 (TSCI + HBO + ADMSCs), euthanized and spinal-cord tissue was harvested by day 49 after TSCI. The result showed that the protein expressions of oxidative-stress (NOX-1/NOX-2), inammatory-signaling (TLR-4/MyD88/IL-1ß/TNF-α/substance-p), cell-stress signaling (PI3K/p-AKT/p-mTOR) and the voltage gated sodium channel (Nav1.3/1.8/1.9) biomarkers were highest in group 2, lowest in group 1 and signicantly lower in group 5 than in groups 3/4 (all p<0.0001), but they did not differ between groups 3/4. The spinal cord-damaged area, the cellular levels of inammatory/DNA-damaged (CD68+/GFAP+/γ-H2AX+ cells), MAPK family biomarkers (p-P38/p-JNK/p-ERK1/2) and cellular expressions of voltage gated sodium channel (Nav.1.3, Nav.1.8 and Nav.1.9 in NF200+ cells) as well as the pain facilitated cellular expressions (p-P38+/peripherin+ cells, p-JNK+/peripherin+ cells, p-ERK/NF200+ cells) exhibited an identical pattern of inammation, whereas the neurological integrity displayed an opposite pattern of inammation among the groups (all p<0.0001). Conclusion: Combined HBO-ADMSCs therapy offered additional benets for protecting the neurological architectural and functional integrity against acute TSCI. procedure repeated eight times within an hour, and immunoreactive bands visualized by enhanced after exposure to Biomax For quantication, ECL signals were digitized using Labwork software (UVP).


Introduction
The productive young age group is the major contributors of the traumatic spinal cord injury (TSCI) patient [1]. The impact of injury not only causes a life-long disability and productivity hindrance, but also compromises the social-interaction physically and emotionally. The annual loss in social economy due to TSCI is highly unacceptable and the incidence of the associated negative-social event is also growing [2][3][4]. Moreover, the life expectancy of TSCI patient is established to be notably reduced as their nancial hardship and demanding pressure on family support [5].
An effective way to improve function of those TSCI patient is urgent and worthy, but it is regrettably still lacking, especially in consideration of the preservation of the residual neurological function and increase in the functional recovery are the two main goals for those patients. Currently, it is a formidable challenge for physicians to achieve these missions [6][7][8][9].
Cellular destruction at the instant of the application of mechanical force is known as primary injury and the subsequent death was determined during the moment of injury. Even the remainder of cells survive under the primary injury, they still doom to death because of coming consequence. Endothelial leakage, apoptosis/death as a result of vascular endothelial cell damage [10] by reactive oxygen species (ROS) [11] and mitochondria dysfunction [12], and cytokine release by neutrophil in situation of in ammatory reaction will further induce local water retention, cell swelling as alternation of cell-membrane water channel dysfunction [13,14], which in turn causes tissue edema, produces compression pressure to the local microcirculation, and further reduce tissue perfusion, i.e., a vicious cycle formation. This vicious cycle which enhances local ischemia and ampli es in ammation, resulting in even more ROS production and cell apoptosis, well known as secondary injury, is one of the main themes to be overcome [15]. An unacceptable high prevalence (i.e., estimated to be over 60~80%) of neuropathic pain is one of notorious outcome in TSCI patients. Regrettably, the treatment for this neuropathic pain is rarely successful [16].
Additionally, joint deformity, muscle atrophy, and bladder and bowel dysfunction always cause the resting life of TSCI patient miserable and dark.
Plentiful data from experimental and clinical trials have shown that cell therapy effectively improved ischemia-related organ dysfunction [17][18][19][20]. The underlying mechanism for improving the organ dysfunction after cell therapy has been proposed to be that the stem cells, especially those of adiposemesenchymal stem cells (ADMSCs), have capacities of anti-in ammation, immunomodulation, suppression of oxidative stress/ROS and tissue regeneration [21][22][23]. Interestingly, our previous studies have further demonstrated that ADMSCs therapy signi cantly improved the neurological function and markedly reduced infarct volume in rat after acute ischemic stroke [24,25]. However, whether stem cell therapy for spinal cord injury would improve the outcome is still rarely addressed [26].
Hyperbaric oxygen (HBO) therapy is a traditional therapy for patients with ischemic peripheral arterial occluded disease [27][28][29]. The underlying mechanism of HBO therapy involved in improving ischemic organ has been proposed to be mainly through an increase of vascular wall permeability and productions of hypoxia-inducible factor-1α (HIF-1α) and stromal cell-derived factor (SDF)-1α that enhance the angiogenesis and blood ow in the ischemic area [29] as well as the mobilization of stem cell into the ischemia region [30]. Additionally, our recent study has further shown that HBO therapy rescued the rat limb from critical limb ischemia injury mainly through anti-in ammation and reduction of the oxidative stress [30]. Based on the aforementioned issues, we proposed that HBO-assisted ADMSCs might offer a synergic effect for rescuing the spinal cord function and improving the neurological outcome against acute TSCI in rat.

Animal model of acute traumatic spinal cord injury
Pathogen-free, adult-male Sprague-Dawley (SD) rats (n=40) weighing 300-325 g (Charles River Technology, BioLASCO Taiwan Co. Ltd., Taiwan) were utilized in the present study. Animals were anesthetized by inhalational 2.0% iso urane and placed in prone position on a warming pad at 37 °C for laminectomy. Sham-operated control (SC) animals underwent opening the skin and muscle layers only, while acute TSCI groups received acute TSCI procedure. The step-by-step procedure for creating an animal model of acute TSCI is listed below: 1. The rats were anesthetized by inhalation of 2.0 % iso urane, then placed in prone position on a warming pad at 37 °C to secure the head and shave the back hair.
2. Skin incision was carried out at the T10~T12 levels, subperiosteal dissection of the paraspinal muscle, and then a laminectomy was performed at T10 to T12 to expose the spinal cord.
3. A modified aneurysm clip was used to create an impact-compression injury of the spinal cord. The clip was left for compressing the spinal cord for 30 seconds prior to be removed by the operator.
4. After induction of the aforementioned TSCI, the muscles were sutured using 3-0 vicryl suture (Ethicon, Somerville, NJ) and the skin/muscle layers were nally closed with Michel clips.
5. The animals recovered from anaesthesia in a portable animal intensive care unit (ThermoCare ® ) for 24 hours, then were allowed to recover in a cage. Bladders were expressed 3 times daily until spontaneous voiding occurred, and any hematuria or urinary tract infection was treated with ampicillin (100 mg/kg by subcutaneous administration twice daily for 5 days).
. Isolation of adipose tissue for culturing mesenchymal stem cells For preparation of allogenic ADMSCs, additional 12 rats were utilized in the present study. The procedure and protocol have been clearly described in our previous reports [24,25]. Brie y, rats in groups 4 and 5 were anesthetized with inhalational 2.0% iso urane 14 days before TSCI procedure for harvesting the adipose tissue surrounding the epididymis. The tissue was then be cut into < 1 mm 3 pieces using a pair of sharp, sterile surgical scissors. Sterile saline (37˚C) was added to the homogenized adipose tissue in a ratio of 3:1 (saline: adipose tissue) by volume, followed by the addition of stock collagenase solution to a nal concentration of 0.5 U/mL. The cells obtained were placed to the rocker for incubation. The contents of the ask were transferred to 50 mL tubes after digestion, followed by centrifugation at 600 g for 5 minutes at room temperature. The ow-through was pipetted to a 40 mm lter into a new 50 mL conical tube. The tubes were centrifuged for a third time at 600 g for 5 minutes at room temperature. The cells were resuspended in saline again. Isolated ADMSCs were cultured in a 100 mm diameter dish with 10 mL DMEM culture medium containing 10% FBS for 14 days.
Basso, Beattie, Bresnahan (BBB) functional scale for assessment of the locomotor capacity of rats after acute TSCI The animals were followed for 7 weeks (i.e., 49 days) after acute TSCI procedure. The procedure and protocol of BBB scale were based on the previous reports [31,32]. In detail, after the aforementioned period each rat was placed in an 80 x 80 x 30 cm 3 clear box lined with a blue non-slippery material and stimulated to move freely. Their movement was video-recorded. Identical copies of the edited videos were given to two independent evaluators who were blinded with regards to the degree of injury severity. Each evaluator made a determination of the locomotor capacity of the rats using the BBB functional scale. Finally, the parameters in each rat were calculated by averaging scores collected from these two evaluators.

Hyperbaric oxygen (HBO) therapy
The procedure and protocol of HBO therapy were based on a recent report [30]. Brie y, to induce tissuelevel hyperoxia, SD rats were subjected to HBO administration in an animal tabletop chamber (Piersol-Dive, model 4934) with the animals exposed to 100% oxygen at 2.4 atmospheres absolute (ATA) for 90 minutes one session for 14 consecutive days.

Western blot analysis
The spinal cord from the rats of the sham control and experimental groups were harvested as previously (1:2000, Cell Signaling) was used as the secondary antibody for one hour incubation at room temperature. The washing procedure was repeated eight times within an hour, and immunoreactive bands were visualized by enhanced chemiluminescence (ECL; Amersham Biosciences) after exposure to Biomax L lm (Kodak). For quanti cation, ECL signals were digitized using Labwork software (UVP).

Histological quanti cation of spinal cord injured area
The H.E. stain was used for identifying the area of spinal cord injury. The spinal cord specimens were sectioned at 4 µm for light microscopy for each animal. Three spinal cord sections from each rat were analyzed and three randomly selected high-power elds (HPFs; 10x) were examined in each section.The integrated area (µm 2 ) of spinal cord injury on each section was calculated using the Image Tool 3 (IT3) image analysis software (University of Texas, Health Science Center, San Antonio, UTHSCSA; Image Tool for Windows, Version 3.0, USA). Three randomly selected HPFs (10x) were analyzed in each section. After assessment of the number of pixels in each injured area per HPF, the numbers of pixels obtained from three HPFs were summed. The procedure was repeated in two other sections for each animal. The mean pixel number per HPF for each animal was then calculated by summing up all pixel numbers and divided by 9. The mean integrated area (µm 2 ) (i.e., the morphological destructive area) of per HPF was obtained using a conversion factor of 19.24 (1 µm 2 corresponded to 19.24 pixels).

Statistical Analysis
Quantitative data were expressed as mean ± SD. Statistical analysis was adequately performed by one way ANOVA followed by Bonferroni multiple-comparison post hoc test. Statistical analysis was performed using SAS statistical software for Windows version 8.2 (SAS institute, Cary, NC, USA). A probability value <0.05 was considered statistically signi cant.

Results
The time courses of neurological function after TSCI procedure ( Figure 1) The BBB functional scale for assessment of the locomotor capacity of rats was performed for each rat on days 1, 3, 7, 14, 28, 35, 42 and 49 after acute TSCI induction. By days 1 and 3, the BBB score of left or right lower limb was signi cantly higher in group 1 (i.e., SC) than in groups 2 (i.e., TSCI), 3 (i.e., TSCI + HBO), 4 (i.e., TSCI + ADMSCs) and 5 (i.e., TSCI + HBO + ADMSCs), but it did not differ among groups 2 to 5 at these time points . Additionally, by days 7 to 49 (i.e., the end of study period), this parameter was still persistently higher in group 1 than that of those groups 2 to 5 with respect to either left or right lower limb. However, as compared with groups 2 and 4, this parameter of left lower limb was signi cantly higher in groups 3 and 5 and signi cantly higher in group 5 than in group 3 but it showed no difference between groups 2 and 4. On the other hand, this parameter of right lower limb was signi cantly higher in groups 3 and 5 than in groups 2 and 4, but it was similar between the former two and between the later two groups. By days 14 to 28, this parameter of left and right lower limbs was further signi cantly improved in groups 3 to 5 than in that of group 2.
Furthermore, this BBB score was signi cantly higher in group 5 than in that of groups 3 and 4 after day 28, but it did not differ between groups 3 and 4 up to the time point of day 49 after acute TSCI procedure. These ndings implicated the attainment of a steady state of neurological functional improvement after days 7 to 49 following TSCI procedure among groups 3, 4 and 5 animals as compared with group 2. Additionally, the combined therapy was found to be superior to either one alone for improvement of neurological function after TSCI procedure.
The protein expressions of oxidative stress and upstream/downstream in ammatory signalings in spinal cord tissue by day 49 after TSCI procedure ( Figure 2) To further elucidate the oxidative stress and in ammatory signaling pathways, the Western blot was utilized in the present study. The result demonstrated that the protein expressions of NOX-1 and NOX-2, two in ammatory indicators, were highest in group 2, lowest in group 1, signi cantly lower in group 5 than in groups 3 and 4 and lower in group 3 than in group 4. Additionally, the protein expressions of TLR-4, Mal, MyD88, TRAF6, IKK-α, IKK-ß, NF-κB, IL-1ß, TNF-α and substance-p, 10 indices of up-and downstream in ammatory signaling pathways, displayed an identical pattern of oxidative stress among the groups.
The protein expressions of cell-stress signaling and voltage gated sodium channel in spinal cord tissue by day 49 after TSCI procedure (Figure 3) We also utilized the Western blot to evaluate the cell stress signaling in the current study. As we expected, the protein expressions of PI3K, phosphorylated (p)-AKT and p-mTOR, three indicators of cell stress signaling, and the protein expressions of Nav1.3, Nav1.8 and Nav1.9, three indicators of the voltage gated sodium channel, were highest in group 2, lowest in group 1, signi cantly lower in group 5 than in groups 3 and 4 and lower in group 3 than in group 4.
The cellular levels of in ammationin spinal cord tissue by day 49 after TSCI procedure (Figure 4) Not only the protein level but also the cellular level of in ammation were determined in the present study. The IF microscopic nding demonstrated that the cellular expressions of CD68 and GFAP, two indicators of in ammation, and cellular expression of γ-H2AX, an indicator of DNA-damaged biomarker, were highest in group 2, lowest in group 1, signi cantly lower in group 5 than in groups 3 and 4 and lower in group 3 than in that of group 4.
The cellular expressions of MAPK family biomarkersin spinal cord tissue by day 49 after TSCI procedure ( Figures 5, 6 and 7) The expressions of p-P38 (Fig. 5) and p-JNK (Fig. 6) in peripherin+ cells (i.e., double staining), and ERK1/2 (Fig. 7) in NF200+ cells, three indicators of pain facilitated cellular expressions, were highest in group 2, lowest in group 1, signi cantly lower in group 5 than in groups 3 and 4 and lower in group 3 than in group 3.
Thecellular expressions of voltage gated sodium channel in spinal cord tissue by day 49 after TSCI procedure (Figures 8, 9 and 10) It is well recognized that voltage gated sodium channel plays an essential role for ectopic discharges or activities in response to stimulation/damage [33,34]. In the present study, we found that the expressions of Nav1.3 (Fig. 8), Nav1.8 (Fig. 9) and Nav1.9 (Fig. 10) colocalized with NF200+ cells, three indicators of voltage gated sodium channel, were highest in group 2, lowest in group 1, signi cantly lower in group 5 than in groups 3 and 4 and lower in group 3 than in group 4.
The spinal cord damaged areaby day 49 after TSCI procedure (Figure 11) Finally, the microscopic nding of H.E. stain revealed that the spinal cord damaged area (longitudinal sections) was highest in group 2, lowest in group 1, signi cantly lower in group 5 than in groups 3 and 4 and signi cantly lower group 3 than in group 4.

Discussion
This study which investigated the therapeutic impact of HBO-ADMSCs on protecting the neurologic function (i.e., locomotor capacity) and architectural integrity of spinal cord yielded several striking implications. Frist, we successfully created an animal model of acute TSCI that was reproducible for different treatment strategies. Second, the underlying mechanism of how TSCI acting on damaging the spinal cord had been clearly delineated. Third, we proved that combined HBO-ADMSCs was superior to either HBO or ADMSCs for protecting the integrities of spinal cord architecture and neurological function, suggesting the combined therapy offered a synergic effect.
Currently, an effective way to improve neurological function in patients after TSCI is still regrettably lacking, suggesting that it remains a formidable challenge for physicians to achieve this mission [6-9]. The most important nding in the present study was that the locomotor capacity (i.e., evalauted by BBB score) was signi cantly impaired in TSCI group than in SC counterpart. However, as comapared with TSCI only, this parameter was remarkably recovered in TSCI animals after receiving HBO or ADMSCs and furthermore remarkably recovered in HBO-ADMSCs treatment, suggesting that combined HBO-ADMSCs offered additional bene ts for the animals. When looking at the integrity of architecture of spinal cord undergoing the microsocpic examination, we found that the anatomical structure of the spinal cord remarkably devastated in TSCI group than in SC group, while notably preserved in HBO or ADMSCs treated and moreover in HBO-ADMSCs treated TSCI animals. This nding could, at least in part, explain why the neurological function was signi cantly improved in that of HBO-ADMSCs treated animals and highlight that this combined therapy may pose as a therapeutic potential for the TSCI patients, especially those who are refractory to conventional therapy.
An association between organ damage and vigorous in amamtory reaction as well as generation of oxidaitve stress which in turn, causes furthermore organ/tissue damage, resulting in organ failure has been well investigated [20][21][22][23][24][25]. In the present study, we found that the cellular-molecular levels of in amamtory biomarkers were substantially increased in TSCI animals than in those of the SC animals.
In this way, our ndings were consistent with the ndings of the previous studies [20][21][22][23][24][25]. Of distnictive nding was that HBO or ADMSCs therapy markedly supressed and combined these two regimens further markedly supressed these molecular-cellular pertubations. Of particularly distinctive nding was that not only the successful treatment was identi ed but also the upstream and downstream in ammatory signalings that participated in the spinal cord damage were clearly clari ed in the present study (refer to Fig. 12). Accordingly, our nding highlights that the identi ed signaling pathway may be served as an useful reference when some medications will be considered as an accessory strategic management for those TSCI patients who are refractory to traditional management.
Cell stress signlaing is commonly elicited in setting of ischemia-reperfusion (IR) condition that has been identi ed to be strongly predictive of unfavorable outcome after organ IR injury [35]. An essential nding in the present study was that as compared with the SC group, the cell stress signaling was remarkably upregulated in TSCI group. In this way, our nding was consistent with the nding of previous study [35]. Intriguingly, the expression of this signaling pathway was signi cantly downregulated by HBO or ADMSCs therapy and further signi cantly downregulated by combining these two regimens.
It is well recognized the protein and cellular levels of voltage gated sodium channel [36] and those of members of mitogen activated protein kinases (MAPKs), such as p38, ERK, and JNK (i.e., MAPK family) [37], have all participated in the pain propagation and facilitation of pain sensation. A principal nding in the present study was that these aforementioned parameters were notably increased in TSCI animals than in those of SC animals. The results of our and previous [36,37] studies could explain why the majority of TSCI patients frequently encounter intractable pain in their lives. Of important nding in the present study was that those parameters of voltage gated sodium channel and MAPK family in injured spinal cord were signi cantly attenuated by HBO or ADMSCs therapy and further signi cantly attenuated by combined HBO-ADMSCs treatment. Our ndings could be considered as an alternative strategic management for those TSCI patients suffering intractable pain refractory to conventional management.

Study limitation
Despite the results were attractive and promising, our study has limitations. First, without stepwise titrating the dose of ADMSCs and the duration of HBO therapy, we did not know which dosage/duration were the most suitable for the animals. Second, the data showed that HBO therapy appeared to be better than that of the ADMSCs therapy. However, perhaps the different duration of therapy might be one of the reasons to explain this phenomenon. Third, despite the neurological function was notably improved by day 49, i.e., the end of the study period, the long-term outcome of these strategic management remains uncertain.
In conclusion, the results of the present study demonstrated that HBO-ADMSCs therapy effectively protected the spinal cord against acute TSCI in rodent.

Availability of data and materials
The data that support the ndings of this study are available from the corresponding authors upon reasonable request.
Ethics approval and consent to participate         Immuno uorescent (IF) microscopic nding of colocalization of Nav1.9 and NF200 in spinal cord neurons by day 49 after TSCI procedure A to E) Illustrating the IF microscopic nding (200x) of positively stained Nav1.9 in spinal neurons (red color spots). F to J) Illustrating the IF microscopic nding (200x) of positively stained NF200 cells (green color). K to O) Illustrating the IF microscopic nding (200x) of merged positively-stained Nav1.9 and NF200 (green-red colocalization). P) Analytical results of number of Nav1.9+/NF200+ cells, * denotes statistical signi cance vs. other groups with different symbols ( †, ‡, §), p<0.0001. All scale bars in lower right corner represent 20µm. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n=6 for each group).

Figure 11
The spinal cord damaged area by day 49 after TSCI procedure A to E) Illustrating the microscopic nding