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Research article
Fenglan Zhang
Qindao University Medical College Affiliated Yantai Yuhuangding Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4426-0907
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Background: Posterior capsule opacification (PCO) occurs frequently following surgical treatment of cataracts, representing a common adverse outcome. PCO develops as a result of lens epithelial cell (LEC) transdifferentiation, migration, and proliferation. In the present report we sought to explore the role of microRNA (miR)-19a in this process and to establish the underlying molecular mechanisms. Methods: miR-19a was transduced into HLE-B3 LECs, with microscopy and RT-qPCR used to confirm transfection efficiency. We then employed MTT, wound healing, and transwell assay approaches to monitor the proliferation and migration of these LECs. We further assessed levels of the proteins PTEN, AKT, and phosphorylated AKT (pAKT) via western blotting in WT and miRNA-transfected cells. Results: HLE‑B3 proliferation was markedly enhanced by miR-19a transduction, as well the migration activity of these cells (both P<0.01). Furthermore, overexpressing miR‑19a failed to reduce PTEN expression whereas it did enhance pAKT levels within these LECs (P<0.05). Conclusions: This suggests that miR-19a can enhance LEC proliferation and migratory activity through a mechanism that may be linked with regulating AKT activation and signaling, thus highlighting a potential avenue for therapeutic treatment of PCO patients.
Keywords
miR-19a, Lens epithelial cells, Proliferation, Migration
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Fenglan Zhang
Qindao University Medical College Affiliated Yantai Yuhuangding Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4426-0907
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 30 Aug, 2019
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Posterior capsule opacification (PCO) occurs frequently following surgical treatment of cataracts, representing a common adverse outcome. PCO develops as a result of lens epithelial cell (LEC) transdifferentiation, migration, and proliferation. In the present report we sought to explore the role of microRNA (miR)-19a in this process and to establish the underlying molecular mechanisms. Methods: miR-19a was transduced into HLE-B3 LECs, with microscopy and RT-qPCR used to confirm transfection efficiency. We then employed MTT, wound healing, and transwell assay approaches to monitor the proliferation and migration of these LECs. We further assessed levels of the proteins PTEN, AKT, and phosphorylated AKT (pAKT) via western blotting in WT and miRNA-transfected cells. Results: HLE‑B3 proliferation was markedly enhanced by miR-19a transduction, as well the migration activity of these cells (both P<0.01). Furthermore, overexpressing miR‑19a failed to reduce PTEN expression whereas it did enhance pAKT levels within these LECs (P<0.05). Conclusions: This suggests that miR-19a can enhance LEC proliferation and migratory activity through a mechanism that may be linked with regulating AKT activation and signaling, thus highlighting a potential avenue for therapeutic treatment of PCO patients.
Figure 1
Figure 2
Figure 3
Figure 4
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