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Research Article
Shima Gharibi
Isfahan University of Medical Science
Corresponding Author
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Hypericum perforatum is a valuable medicinal plant with anti-depressant activity. Hypericin is the major compound responsible for such activity. In the present study, the effect of four nano-capsulated hormones of 2,4-epibrassinolide, spermidine, salycilic acid and cycocel were investigated on the amount of hypericin based on HPLC analysis in two locations (Saman and Isfahan). For each hormone, the normal form was also compared with nano form. The expression patterns of the key genes (Hyp-1, pks1, pks2) for hypericin production was also evaluated using qRT-PCR. Moreover, GC-MS analysis was also performed for determination of the compounds in studied treatments. The major compounds were germacrene D (3.29–33.53%), β-caryophyllene (0-4.08) and α-longipinene (0-24.05%). In most cases, nano-hormones led to increase in these components. Significant changes were obtained in expression of key genes in hypericin synthesis as a result of nano-hormones treatments in Isfahan site. Overall, nano-hormones revealed higher increase in expression of all genes as compared with normal hormones in this site. The expression of Hyp-1, Pks1 and Pks2 was significantly increased using spermidine, 24-epibrassinolide and cycocel in Isfahan location in both nano-hormones and normal ones, while the expression of Hyp-1 was decreased in SA treatment in Isfahan location. Based on HPLC analysis, hypericin ranged from 0.21 in control to 0.51 mg 100 g− 1 DW in nano-SA in Isfahan site. Finally, the expression of the key genes were mostly elevated in colder climates and nano-form formulation.
Keywords
Hypericum, Hypericin, St. John's Wort, nano-capsulated hormones, gene expression, HPLC, GC-MS
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This is a preprint. It has not completed peer review.
Research Article
Shima Gharibi
Isfahan University of Medical Science
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 03 May, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Hypericum perforatum is a valuable medicinal plant with anti-depressant activity. Hypericin is the major compound responsible for such activity. In the present study, the effect of four nano-capsulated hormones of 2,4-epibrassinolide, spermidine, salycilic acid and cycocel were investigated on the amount of hypericin based on HPLC analysis in two locations (Saman and Isfahan). For each hormone, the normal form was also compared with nano form. The expression patterns of the key genes (Hyp-1, pks1, pks2) for hypericin production was also evaluated using qRT-PCR. Moreover, GC-MS analysis was also performed for determination of the compounds in studied treatments. The major compounds were germacrene D (3.29–33.53%), β-caryophyllene (0-4.08) and α-longipinene (0-24.05%). In most cases, nano-hormones led to increase in these components. Significant changes were obtained in expression of key genes in hypericin synthesis as a result of nano-hormones treatments in Isfahan site. Overall, nano-hormones revealed higher increase in expression of all genes as compared with normal hormones in this site. The expression of Hyp-1, Pks1 and Pks2 was significantly increased using spermidine, 24-epibrassinolide and cycocel in Isfahan location in both nano-hormones and normal ones, while the expression of Hyp-1 was decreased in SA treatment in Isfahan location. Based on HPLC analysis, hypericin ranged from 0.21 in control to 0.51 mg 100 g− 1 DW in nano-SA in Isfahan site. Finally, the expression of the key genes were mostly elevated in colder climates and nano-form formulation.
Figure 1
Figure 2
Figure 3
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