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Shiro Murata
Faculty of Veterinary Medicine, Hokkaido University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2569-899X
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Background: Marek’s disease virus (MDV) causes malignant lymphomas in chickens (Marek’s disease, MD). MD is currently controlled by vaccination; however, MDV strains have a tendency to develop increased virulence. Distinct diversity and point mutations are present in the Meq proteins, the oncoproteins of MDV, suggesting that changes in protein function induced by amino acid substitutions might affect MDV virulence. We previously reported that recent MDV isolates in Japan display distinct mutations in Meq proteins from those observed in traditional MDV isolates in Japan, but similar to those in MDV strains isolated from other countries.
Methods: To further investigate the genetic characteristics in Japanese field strains, we sequenced the whole genome of an MDV strain that was successfully isolated from a chicken with MD in Japan. A phylogenetic analysis of the meq gene was also performed.
Results: Phylogenetic analysis revealed that the Meq proteins in most of the Japanese isolates were similar to those of Chinese and European strains, and the genomic sequence of the Japanese strain was classified into the Eurasian cluster. Comparison of coding region sequences among the Japanese strain and MDV strains from other countries revealed that the genetic characteristics of the Japanese strain were similar to those of Chinese and European strains.
Conclusions: The MDV strains distributed in Asian and European countries including Japan seem to be genetically closer to each other than to MDV strains from North America. These findings indicate that the genetic diversities of MDV strains that emerged may have been dependent on the different vaccination-based control approaches.
Keywords
Marek’s disease virus, Marek’s disease, Meq, Japanese strain, whole genome sequencing
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementaryFigure1.pdf
Alignment of the deduced amino acid sequences of the variable regions of UL36 proteins. The variable regions in the UL36 proteins from Kgs-c1 and MDV strains isolated in other countries were aligned. Shaded fields in gray indicate the repeat sequence of “KP (T/S/P)PA(S/P)”, and a solid line indicates each repeat sequence. The different colors of the letters indicate each type of the repeat sequence of “KP (T/S/P)PA(S/P)”. Patterned fields indicate the repeat sequence of “KPKPPP(D/A/T)PD(F/S)”, and a dotted line indicates each repeat sequence. The number of each repeat sequence is summarized in Supplementary Table 1.
- Supplementarytable1.pdf
The meq genes used for analyses in this study.
- Supplementarytable2.docx
Number of repeat sequences in the UL36 proteins.
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CURRENT STATUS: Published
View the published article at Virology Journal.
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Editorial decision: Accept
On 09 Nov, 2020
Editor assigned
On 08 Nov, 2020
Submission checks complete
On 08 Nov, 2020
Editor invited
On 08 Nov, 2020
Version 1
Posted 23 Jul, 2020
No comments provided
Review #4 received
Received 11 Oct, 2020
Editorial decision: Minor revision
On 11 Oct, 2020
Review #3 received
Received 09 Oct, 2020
Review #2 received
Received 07 Oct, 2020
Review #1 received
Received 26 Sep, 2020
Reviewer #1 agreed
On 25 Sep, 2020
Reviewer #2 agreed
On 25 Sep, 2020
Reviewer #3 agreed
On 25 Sep, 2020
Reviewer #4 agreed
On 25 Sep, 2020
Reviewers invited
Invitations sent on 24 Sep, 2020
Editor assigned
On 23 Jul, 2020
Editor invited
On 22 Jul, 2020
Submission checks complete
On 20 Jul, 2020
First submitted
On 17 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Virology
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Virology Journal.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Shiro Murata
Faculty of Veterinary Medicine, Hokkaido University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2569-899X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Virology Journal.
No community comments so far
Editorial decision: Accept
On 09 Nov, 2020
Editor assigned
On 08 Nov, 2020
Submission checks complete
On 08 Nov, 2020
Editor invited
On 08 Nov, 2020
Version 1
Posted 23 Jul, 2020
No comments provided
Review #4 received
Received 11 Oct, 2020
Editorial decision: Minor revision
On 11 Oct, 2020
Review #3 received
Received 09 Oct, 2020
Review #2 received
Received 07 Oct, 2020
Review #1 received
Received 26 Sep, 2020
Reviewer #1 agreed
On 25 Sep, 2020
Reviewer #2 agreed
On 25 Sep, 2020
Reviewer #3 agreed
On 25 Sep, 2020
Reviewer #4 agreed
On 25 Sep, 2020
Reviewers invited
Invitations sent on 24 Sep, 2020
Editor assigned
On 23 Jul, 2020
Editor invited
On 22 Jul, 2020
Submission checks complete
On 20 Jul, 2020
First submitted
On 17 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Virology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Virology Journal.
Background: Marek’s disease virus (MDV) causes malignant lymphomas in chickens (Marek’s disease, MD). MD is currently controlled by vaccination; however, MDV strains have a tendency to develop increased virulence. Distinct diversity and point mutations are present in the Meq proteins, the oncoproteins of MDV, suggesting that changes in protein function induced by amino acid substitutions might affect MDV virulence. We previously reported that recent MDV isolates in Japan display distinct mutations in Meq proteins from those observed in traditional MDV isolates in Japan, but similar to those in MDV strains isolated from other countries.
Methods: To further investigate the genetic characteristics in Japanese field strains, we sequenced the whole genome of an MDV strain that was successfully isolated from a chicken with MD in Japan. A phylogenetic analysis of the meq gene was also performed.
Results: Phylogenetic analysis revealed that the Meq proteins in most of the Japanese isolates were similar to those of Chinese and European strains, and the genomic sequence of the Japanese strain was classified into the Eurasian cluster. Comparison of coding region sequences among the Japanese strain and MDV strains from other countries revealed that the genetic characteristics of the Japanese strain were similar to those of Chinese and European strains.
Conclusions: The MDV strains distributed in Asian and European countries including Japan seem to be genetically closer to each other than to MDV strains from North America. These findings indicate that the genetic diversities of MDV strains that emerged may have been dependent on the different vaccination-based control approaches.
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementaryFigure1.pdf
Alignment of the deduced amino acid sequences of the variable regions of UL36 proteins. The variable regions in the UL36 proteins from Kgs-c1 and MDV strains isolated in other countries were aligned. Shaded fields in gray indicate the repeat sequence of “KP (T/S/P)PA(S/P)”, and a solid line indicates each repeat sequence. The different colors of the letters indicate each type of the repeat sequence of “KP (T/S/P)PA(S/P)”. Patterned fields indicate the repeat sequence of “KPKPPP(D/A/T)PD(F/S)”, and a dotted line indicates each repeat sequence. The number of each repeat sequence is summarized in Supplementary Table 1.
- Supplementarytable1.pdf
The meq genes used for analyses in this study.
- Supplementarytable2.docx
Number of repeat sequences in the UL36 proteins.
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