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Research article
Jian Gao
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8948-2613
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
E2F/DP proteins have been shown to regulate genes implicated in cell cycle control and DNA repair. However, to date, research into the potential role of Moso bamboo E2F/DP family has been limited.
Results
Here, we identified 24 E2F/DPs in the Moso bamboo genome including nine E2F, six DP, eight DEL and one gene with partial E2F domain. Estimation of the divergence time of paralogous gene pairs suggested that E2F/DP family expansion was mainly contributed by whole-genome duplication event. The regulatory element and coexpression network analysis indicated that E2F/DP regulated the expression of cell cycle-related genes. Yeast two hybrid assay and expression analysis based on transcriptome data and in situ hybridization indicated that PheE2F-PheDP complex played important roles in winter moso bamboo shoot growth. qRT-PCR results showed that PheE2F/DPs performed diverse expression patterns in response to drought and salt treatment and diurnal cycles.
Conclusion
Our findings provide novel insights into the Moso bamboo E2F/DP family and partial experimental evidence for further functional verification of the PheE2F/DPs.
Keywords
E2F/DPs, Moso bamboo, expression, cell cycle, bamboo shoot
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Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Plant Biology.
No community comments so far
Editorial decision: Minor revision
On 06 Feb, 2021
Reviewer #2 agreed
On 12 Jan, 2021
Reviewer #1 agreed
On 12 Jan, 2021
Review #1 received
Received 12 Jan, 2021
Editor assigned
On 02 Jan, 2021
Reviewers invited
Invitations sent on 02 Jan, 2021
Editor invited
On 02 Jan, 2021
Submission checks complete
On 10 Dec, 2020
Version 2
Posted 14 Aug, 2020
No comments provided
Editorial decision: Major revision
On 03 Nov, 2020
Reviewer #3 agreed
On 28 Oct, 2020
Review #3 received
Received 28 Oct, 2020
Review #2 received
Received 27 Oct, 2020
Reviewer #2 agreed
On 21 Oct, 2020
Review #1 received
Received 11 Sep, 2020
Reviewer #1 agreed
On 17 Aug, 2020
Reviewers invited
Invitations sent on 14 Aug, 2020
Editor assigned
On 12 Aug, 2020
Submission checks complete
On 11 Aug, 2020
Editor invited
On 11 Aug, 2020
No comments provided
Editorial decision: Revise before sending to peer reviewers
On 31 Jul, 2020
Editor assigned
On 23 Jul, 2020
First submitted
On 22 Jul, 2020
Submission checks complete
On 22 Jul, 2020
Editor invited
On 22 Jul, 2020
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Plant Biology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Jian Gao
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8948-2613
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Plant Biology.
No community comments so far
Editorial decision: Minor revision
On 06 Feb, 2021
Reviewer #2 agreed
On 12 Jan, 2021
Reviewer #1 agreed
On 12 Jan, 2021
Review #1 received
Received 12 Jan, 2021
Editor assigned
On 02 Jan, 2021
Reviewers invited
Invitations sent on 02 Jan, 2021
Editor invited
On 02 Jan, 2021
Submission checks complete
On 10 Dec, 2020
Version 2
Posted 14 Aug, 2020
No comments provided
Editorial decision: Major revision
On 03 Nov, 2020
Reviewer #3 agreed
On 28 Oct, 2020
Review #3 received
Received 28 Oct, 2020
Review #2 received
Received 27 Oct, 2020
Reviewer #2 agreed
On 21 Oct, 2020
Review #1 received
Received 11 Sep, 2020
Reviewer #1 agreed
On 17 Aug, 2020
Reviewers invited
Invitations sent on 14 Aug, 2020
Editor assigned
On 12 Aug, 2020
Submission checks complete
On 11 Aug, 2020
Editor invited
On 11 Aug, 2020
No comments provided
Editorial decision: Revise before sending to peer reviewers
On 31 Jul, 2020
Editor assigned
On 23 Jul, 2020
First submitted
On 22 Jul, 2020
Submission checks complete
On 22 Jul, 2020
Editor invited
On 22 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Plant Biology.
Background
E2F/DP proteins have been shown to regulate genes implicated in cell cycle control and DNA repair. However, to date, research into the potential role of Moso bamboo E2F/DP family has been limited.
Results
Here, we identified 24 E2F/DPs in the Moso bamboo genome including nine E2F, six DP, eight DEL and one gene with partial E2F domain. Estimation of the divergence time of paralogous gene pairs suggested that E2F/DP family expansion was mainly contributed by whole-genome duplication event. The regulatory element and coexpression network analysis indicated that E2F/DP regulated the expression of cell cycle-related genes. Yeast two hybrid assay and expression analysis based on transcriptome data and in situ hybridization indicated that PheE2F-PheDP complex played important roles in winter moso bamboo shoot growth. qRT-PCR results showed that PheE2F/DPs performed diverse expression patterns in response to drought and salt treatment and diurnal cycles.
Conclusion
Our findings provide novel insights into the Moso bamboo E2F/DP family and partial experimental evidence for further functional verification of the PheE2F/DPs.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
This is a list of supplementary files associated with this preprint. Click to download.
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