This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Research Article
Debojit Sarma
Assam Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3808-7231
License:
This work is licensed under a CC BY 4.0 License. Read Full License
We carried out the morphological, biochemical, and molecular characterization of twenty cultivars of the least studied Joha (aromatic) rice indigenous to Assam. Unweighted Neighbour Joining (UNJ) clustering based on usual Euclidean distances for thirty-seven polymorphic morphological markers grouped the cultivars into three clusters with eight, eleven, and one genotype. The Joha rice cultivars showed highly significant differences for all the quantitative traits except for panicle length. The genotypic and phenotypic coefficients of variability (GCV & PCV) were high for grain yield ha-1 (24.62 & 24.85%) and filled grains panicle-1 (23.69 & 25.02%). All the traits except days to flowering and maturity, flag leaf breadth, and spikelet fertility exhibited high heritability along with high to moderate genetic advance, indicating the predominant role of additive gene action. Mahalanobis D2 analysis revealed three multi-genotypic and four mono-genotypic clusters of the cultivars. The cultivars' average polyunsaturated fatty acids were 37.9% oleic acid, 39.22% linoleic acid, and 0.5% linolenic acid. The fatty acid profile of Local Joha was superior to the other cultivars as it showed a high level of linoleic and linolenic acid and low saturated fatty acid content. Kon Joha 4 and Ronga Joha contained the highest iron (82.88 mg kg-1) and zinc (47.39 mg kg-1), respectively, while protein content of Kon Joha-1 and amylose content of Harinarayan were the highest. Joha (Bihpuria) showed the highest gel consistency of 140.50 mm. Kalijeera, Kunkuni Joha, Kon Joha-5, Manimuni Joha and Kon Joha-2 accorded a strong aroma. PCR amplified 174 alleles with a mean value of 2.64 across the 66 polymorphic SSR markers. PIC values ranged from 0.091 to 0.698, with an average of 0.326. The highly informative (PIC>0.50) markers were RM316, RM283, RM585, RM1388, RM3562, RM171, R1M30, RM118, RM11and RM29 for identification of the twenty aromatic rice cultivars. The UNJ clustering based on Jaccard's coefficients classified the cultivars into three distinct clusters with eight, ten, and two genotypes. Our study revealed the nutritional richness of these specialty Joha rice cultivars and sufficient scope for yield enhancement through their interbreeding to keep quality intact.
Keywords
Joha rice, DUS characteristics, genetic variability, Mahalanobis D2, fatty acid profile, molecular diversity
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
- Supplementaryfile1.xlsx
Supplementary Table S1: Details of SSR primers used in the study Supplementary Table S2: Usual Euclidean distances among the twenty Joha rice cultivars based on thirty-seven polymorphic morphological characters Supplementary Table S3: A pooled analysis of variance for the characters of the twenty indigenous Joha rice cultivars evaluated during the Sali season of 2018 and 2019 Supplementary Table S4: Mean performance of the twenty indigenous Joha rice cultivars evaluated during Sali season of 2018 and 2019 Supplementary Table S5: WILKS TEST and ANOVA for dispersion Supplementary Table S6: Jaccard’s dissimilarity matrix of the twenty indigenous Joha rice cultivars based on 66 SSR markers
- Tables.docx
Table 1: List of indigenous Joha rice cultivars used in the investigation Table 2: Distribution of the Joha rice cultivars based on monomorphic characteristics Table 3: Distribution of the Joha rice cultivars based on polymorphic characteristics Table 4: Top ranking cultivars with desirable characteristics Table 5: Genetic variability parameters for the traits of the twenty indigenous Joha rice cultivars of Assam evaluated during the Sali season of 2018 and 2019 Table 6: Composition of the Tocher’s clusters based on Mahalanobis D2 analysis Table 7: Intra- (bold) and inter-cluster distances Table 8: Cluster mean for the characters and their contribution to the total variation Table 9: Biochemical characteristics of the twenty Joha rice cultivars Table 10: SSR markers with their number of different alleles amplified (Na), major allele frequency (MAF), number of effective alleles (Ne), Shannon’s informative index (I), observed heterozygosity (Ho), expected heterozygosity (He) and the polymorphic information content (PIC) in the twenty indigenous Joha rice cultivars.
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 2
Posted 19 May, 2021
No community comments so far
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Plant Molecular Biology and Genetics
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Debojit Sarma
Assam Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3808-7231
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 2
Posted 19 May, 2021
No community comments so far
No comments provided
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Plant Molecular Biology and Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
We carried out the morphological, biochemical, and molecular characterization of twenty cultivars of the least studied Joha (aromatic) rice indigenous to Assam. Unweighted Neighbour Joining (UNJ) clustering based on usual Euclidean distances for thirty-seven polymorphic morphological markers grouped the cultivars into three clusters with eight, eleven, and one genotype. The Joha rice cultivars showed highly significant differences for all the quantitative traits except for panicle length. The genotypic and phenotypic coefficients of variability (GCV & PCV) were high for grain yield ha-1 (24.62 & 24.85%) and filled grains panicle-1 (23.69 & 25.02%). All the traits except days to flowering and maturity, flag leaf breadth, and spikelet fertility exhibited high heritability along with high to moderate genetic advance, indicating the predominant role of additive gene action. Mahalanobis D2 analysis revealed three multi-genotypic and four mono-genotypic clusters of the cultivars. The cultivars' average polyunsaturated fatty acids were 37.9% oleic acid, 39.22% linoleic acid, and 0.5% linolenic acid. The fatty acid profile of Local Joha was superior to the other cultivars as it showed a high level of linoleic and linolenic acid and low saturated fatty acid content. Kon Joha 4 and Ronga Joha contained the highest iron (82.88 mg kg-1) and zinc (47.39 mg kg-1), respectively, while protein content of Kon Joha-1 and amylose content of Harinarayan were the highest. Joha (Bihpuria) showed the highest gel consistency of 140.50 mm. Kalijeera, Kunkuni Joha, Kon Joha-5, Manimuni Joha and Kon Joha-2 accorded a strong aroma. PCR amplified 174 alleles with a mean value of 2.64 across the 66 polymorphic SSR markers. PIC values ranged from 0.091 to 0.698, with an average of 0.326. The highly informative (PIC>0.50) markers were RM316, RM283, RM585, RM1388, RM3562, RM171, R1M30, RM118, RM11and RM29 for identification of the twenty aromatic rice cultivars. The UNJ clustering based on Jaccard's coefficients classified the cultivars into three distinct clusters with eight, ten, and two genotypes. Our study revealed the nutritional richness of these specialty Joha rice cultivars and sufficient scope for yield enhancement through their interbreeding to keep quality intact.
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
- Supplementaryfile1.xlsx
Supplementary Table S1: Details of SSR primers used in the study Supplementary Table S2: Usual Euclidean distances among the twenty Joha rice cultivars based on thirty-seven polymorphic morphological characters Supplementary Table S3: A pooled analysis of variance for the characters of the twenty indigenous Joha rice cultivars evaluated during the Sali season of 2018 and 2019 Supplementary Table S4: Mean performance of the twenty indigenous Joha rice cultivars evaluated during Sali season of 2018 and 2019 Supplementary Table S5: WILKS TEST and ANOVA for dispersion Supplementary Table S6: Jaccard’s dissimilarity matrix of the twenty indigenous Joha rice cultivars based on 66 SSR markers
- Tables.docx
Table 1: List of indigenous Joha rice cultivars used in the investigation Table 2: Distribution of the Joha rice cultivars based on monomorphic characteristics Table 3: Distribution of the Joha rice cultivars based on polymorphic characteristics Table 4: Top ranking cultivars with desirable characteristics Table 5: Genetic variability parameters for the traits of the twenty indigenous Joha rice cultivars of Assam evaluated during the Sali season of 2018 and 2019 Table 6: Composition of the Tocher’s clusters based on Mahalanobis D2 analysis Table 7: Intra- (bold) and inter-cluster distances Table 8: Cluster mean for the characters and their contribution to the total variation Table 9: Biochemical characteristics of the twenty Joha rice cultivars Table 10: SSR markers with their number of different alleles amplified (Na), major allele frequency (MAF), number of effective alleles (Ne), Shannon’s informative index (I), observed heterozygosity (Ho), expected heterozygosity (He) and the polymorphic information content (PIC) in the twenty indigenous Joha rice cultivars.
Loading...