DEVELOPMENT OF ORGANIC BRIQUETTES FROM SLAUGHTERHOUSE WASTE AS NUTRIENT SOURCE FOR PLANT GROWTH

A study was conducted to develop organic briquettes using biomass originating from slaughterhouse wastes and to investigate its potential as a nutrient source for plant growth. Dried rumen contents were mixed with fresh blood collected after bleeding of animals in different proportions and dried using tray drier. The dried rumen content-blood (RB) mixture was further mixed with commercially available coir pith in 12 different proportions. The rumen content-blood-coir pith (RBC) mixture was formed into briquettes using a hydraulic biomass briquetting machine. Each organic briquette was 5 cm in diameter and had an average weight of 30-40 g. The treatments were analyzed for pH, moisture content, electrical conductivity (EC), organic carbon (OC), total nitrogen, carbon: nitrogen ratio, total phosphorus and total potassium. Organic briquette having rumen content with 100 % blood and 80 % coir pith (T10) recorded the highest total nitrogen, total phosphorus and total potassium content. The treatments for plant growth studies were selected based on the criteria that each treatment had highest values for at least two of the three primary nutrients of nitrogen, phosphorus and potassium. The treatments T5, T7, T8, T10 and T12 were selected for plant growth studies. Pot experiment using selected briquettes was carried out to determine the effect of selected briquettes on growth parameters and yield attributes of okra (Abelmoschus esculentus). Nutrient release pattern of the selected organic briquettes was also studied. The best desirable growth parameters and yield attributes of okra was observed in T10. Availability of nutrients in the medium were relatively less during first two months of planting (MAP), however, it significantly increased between 3 MAP and 4 MAP, which resulted in better plant growth and fruit yield. The study showed that there is great potential for development of organic briquettes using slaughter house wastes as an eco-friendly process, which are compact, easy to transport and market, and ideal for grow bag cultivation and garden nurseries.

the 12 treatments, the best five treatments for pot experiment were selected based on the criteria of higher nutrient contents for more than two primary nutrients of nitrogen, phosphorus and potassium. Based on this, T5, T7, T8, T10 and T12 were selected for pot experiment.

Effects of organic briquette on crop growth and yield
Effects of organic briquettes made from slaughterhouse wastes on growth and yield of okra (Abelmoschus esculentus) were assessed by pot experiments conducted over a period of five months (February -June, 2020) at Agricultural Research Station, Kerala Agricultural University, Mannuthy.

Soil characteristics
pH and electrical conductivity of the soil were analysed as per Fertilizer Control Order [7]. Available nitrogen of the soil was determined by alkaline permanganometry [10].
Available phosphorus was estimated by extracting the soil samples with Bray No.1 solution followed by colorimetric estimation using reduced molybdate ascorbic acid blue colour method using Spectrophotometer (Model: lambda 25) [11]. The soil samples were extracted with neutral normal ammonium acetate and available potassium was estimated using Flame photometer (Model: CL 308) [12].

Pot experiment
The treatments T5, T7, T8, T10 and T12 had the higher nutrient contents for more than two primary nutrients of nitrogen, phosphorus and potassium were selected for the pot experiment. All the selected combination of briquettes was replicated ten times. The treatments were randomly allocated to the pots following a completely randomized design. Ten replicates of control pots, containing no briquettes were also prepared. Grow bags having size 14" ×16" were used as pots. Potting mixture (soil: perlite in the ratio 1:1 by volume and 10 kg weight) mixed with 10 g of pseudomonas was filled in each pot. Three briquettes each weighing [30][31][32][33][34][35][36][37][38][39][40] g was placed approximately 3 cm below the top layer. Perlite, an inert medium was used in potting mixture to increase the aeration in root zone. Nine days old seedlings of Arka Anamika variety of okra (Abelmoschus esculentus) were planted in each pot. Each pot was irrigated daily using equal quantities of tap water. After 21 days of planting the seedling, the supernatant liquid obtained from the fermentation of ground nut cake with nutrient composition of 7% nitrogen was applied at the rate of 10 ml per plant uniformly to all plants as an additional nutrient supplement.
Morphological parameters such as height of the plant and number of leaves were recorded at one, two, three, four and five months after planting (MAP). The yield attributes of the plants including days to first flowering, number of flowers per plant, number of fruits, length and weight of the fruit from all the plants and fruit yield (kg) per plant was recorded upto 5 MAP.

NUTRIENT RELEASE PATTERN OF ORGANIC BRIQUETTES
Three briquettes each of the selected treatments were placed in separate pots containing potting mixture (soil: perlite in the ratio 1:1 by volume) mixed with 10 gm of pseudomonas at a depth of about 3-4 cm and irrigated daily. Three soil samples per pot, at a depth of approximately 2-4 cm were taken at monthly interval for five months and analyzed for available nitrogen [10], available phosphorus [11] and available potassium [12].

STATISTICAL ANALYSIS
Data recorded were analysed statistically as per [13] by using SPSS Software (Version 24.0). Growth parameters and nutrient release pattern were analysed employing repeated measures ANOVA. Chemical properties of organic briquettes and yield attributes were analysed using one-way ANOVA.

Physico-chemical properties of briquettes
The physico-chemical properties of different treatments of organic briquettes are presented in Table I.

pH
In the present study, various treatments of organic briquettes recorded pH in the range of 6.73 to 8.03. Among the treatments, T8 and T6 had the highest and the lowest pH (8.03±0.09 and 6.73±0.03) as seen in Table 1. The pH of the treatments was in compliance with the range of values prescribed [7] except T3, T4, T7 and T8. The pH of the cattle manure compost was observed to be 7.4 [14] and 7.3 [15], while the estimated pH of rumen contents used for preparing organic fertilizer was 8.0 [16]. The high pH could be attributed to the imbalance of nutrients inside the briquettes [17].

Moisture
The lowest moisture content (6.62±0.15 %) was recorded in T2 and the highest moisture content (9.17±0.08 %) was recorded in T12 as seen in Table 1. The values of T2 were significantly (p<0.05) different from all other treatments while T12 was not significantly different from other treatments except T1 and T2. The moisture content in the present study was in agreement with the values proposed [6][7] and [18] who stated that, moisture content less than 15 % was ideal for briquetting. In another experiment, a high moisture content (> 10 %), resulted in excess steam production, blocking the products from hopper and shooting out briquettes from dies [19].

Electrical conductivity
Electrical conductivity is a measure of salinity of soil. Among the treatments of organic briquettes, T5 had the lowest (0.16±0.01 dS/m) and T8 had the highest (1.19±0.12 dS/m) electrical conductivity as seen in Table 1. Electrical conductivity in the present study was similar to the values reported for cattle manure [20], but was lower than the values reported by [21] for cattle manure. The lower electrical conductivity values of the organic briquettes could be attributed due to the salts lost during drying of the rumen contents or during the washing of coir pith. An electrical conductivity greater than 2 dS/m restricted the growth of sensitive crops [22] and the briquettes used in the study were found to be in safe limit.

Organic carbon
As seen in Table 1, the organic carbon content was the highest for T8 (45.97±0.93 %) and the lowest for T1 (39.24±0.77 %) among the treatments under study. An organic carbon content of 16.6 % was observed for cattle manure compost [15] and 29.32 % in composted animal manure [23], while [24] reported an organic carbon content of 18.22 % in bovine digesta mixed with tannery sludge. The reported values were lower when compared to the organic carbon content of briquettes used in the current study. Organic carbon content of cattle manure was scanty and the inclusion of lignocellulosic materials could equilibrize the under supply of carbon in cattle manure [25]. Coir pith had a high carbon content [26]. The high organic carbon content of the treatments used in the present study could be attributed due to the addition of high carbon content materials like coir pith in high proportions to the dried rumen contentblood mixture.

Total nitrogen
The organic briquettes, T10 and T1 had the highest (1.76±0.09 %) and the lowest (0.92±0.09 %) total nitrogen content respectively as seen in Table 1. The total nitrogen content of the briquettes under study increased with increase in level of the blood added. Cattle manure compost had a total nitrogen content of 0.95 % [15], which was lower than that of the organic briquettes used in the current study. This can be explained on the basis of the blood added during briquetting process which is an excellent protein source as it contains about 95 % protein [3] A high nitrogen content of 5.5 % was observed in bovine blood-rumen digesta mixture in the ratio 3:1 [16], which was higher than the values of organic briquettes used in the present study. The lower nitrogen content observed in the current study could be due to the lower level of incorporation of blood in the rumen contentsblood mixture (1:1).

Carbon: Nitrogen ratio
The C: N ratio was the highest for T1 (43.44±4.50) and the lowest for T10 (24.29±1.05) as seen in Table 1. Organic briquettes made of bovine blood and rumen contents had a C: N ratio of 4.8 [27], while granulated cattle manure compost had a C: N ratio of 17 [28]. The values observed in the present study was higher than the values mentioned in the above reports. A wide C: N ratio in the current study could be due to the addition of high amount of high carbon organic materials like coir pith. Coir pith had a wide C: N ratio of 100:1 [29]. The composting of raw coir pith with cattle manure and poultry waste could reduce the C: N ratio to 31.3 [5].
The C: N ratio is an indication of immediate availability of nutrients and the C: N ration of T10 was found to be favourable for the release of nutrients and thereby uptake of plants.

Total phosphorus
Total phosphorus content was the highest for T10 (1.52±0.07 %) and the lowest for T6 (0.79±0.06 %) as seen in Table 1. The phosphorus content of the organic briquettes used in the study were higher than the minimum recommended levels (0.4 %) prescribed by [7] and dried rumen contents (0.69 %) as reported by [30]. In the current study, higher levels of addition of blood in the rumen contents -blood mixture didn't cause comparable increase in the phosphorus content of the briquettes as blood is not a good source of phosphorus. There was no significant difference in the phosphorus content of rumen contents-blood mixture mixed in 1:1, 2:1 and 3:1 ratio as an organic fertilizer [16].

Total potassium
The potassium content of the organic briquettes was the highest for T10 (1.15±0.11 %) and the lowest for T11 (0.55±0.18 %) respectively as seen in Table 1. The organic fertilizer developed from rumen contents had a potassium content of 0.17 % [24]. The potassium content of bovine blood-rumen digesta mixture used as an organic fertilizer was 0.93 % [16]. These values were considerably lower when compared to that of the potassium content of organic briquettes in the study. Coir pith is material possessing high potassium content [26]. The higher levels of potassium observed in the current study might be due to the incorporation of coir pith in the manufacture of briquettes. With increasing content of coir pith in 12 treatments, there was no comparable increase in the potassium content, though coir pith at four different levels were incorporated in these treatments. This could be due to the process of washing of coir pith which would have resulted in removal of a portion of potassium salts. The washing of coir pith has resulted in reduced the level of potassium salts [4].

Selection of organic briquettes for pot experiment
The treatments T5, T7, T8, T10 and T12 had higher nutrient contents for more than two primary nutrients of nitrogen, phosphorus and potassium for plant growth. Hence these treatments were selected for pot experiment.

Soil characteristics
The soil used for the study was having a pH of 5

Growth parameters
The growth parameters of okra are presented in Table II and Table III.

Height of the plant
As the number of months after planting (MAP) progressed, the height of okra plants cm at 55 DAS. The height of the plants observed in the current study was lower than these reports at two MAP which may be due to a slower release of nutrients readily available for absorption by plant roots during the initial stages due to a wide C: N ratio.

Number of leaves
The number of leaves of okra by application of organic briquettes in the study showed an increasing pattern up to four months after planting and then decreased. Number of leaves showed a similar trend in all the treatments and control. Maximum number of leaves were seen in T10 (55.30±1.90) at four MAP and lowest number of leaves (7.00±0.46) were seen in control at one MAP. The probable reason for increased number of leaves in T10 may be due to the increased supply of photosynthates for growth and due to increased nutrient content compared to other treatments [33]. Application of animal manure in pot culture of okra produced a higher number of leaves (58) in comparison to control where no animal manure was added [34]. The okra var. Arka Anamika had 58.7 leaves on an average at 120 DAS [35]. Both these findings were in line with the results obtained in the present work. The results indicated that manurebased applications could trigger vegetative growth in okra. The increased number of leaves upto 4 MAP indicated that the vegetative growth is maximum upto 4 months and hereafter translocation of photosynthates for fruit production was decreased.

Yield attributes of okra
The yield attributes of okra after the application of organic briquettes are presented in Table IV and V.

Number of fruits per plant
The total number of fruits per plant had been widely considered as the most crucial factor determining the yield of okra. The treatment T10 recorded the highest number of fruits per plant (32.70±1.00) in okra and control recorded the lowest number of fruits per plant (13.50±0.45). Among the treatments applied, T10 had the highest nitrogen content. An increase in nitrogen was directly proportional to fruit production as nitrogen plays a vital role in assimilate production needed for photosynthesis [38]. The number of fruits obtained by the addition of cow dung as an organic nutrient source was 14.35 [39]. Application of farmyard manure at the rate of 10t/ha produced 10.62 fruits on average [35]. The values were slightly lower than the values recorded in the current work, which could be attributed due to the higher availability and uptake of nutrients by plants. The enhanced vegetative growth and higher number of flowers had resulted in higher fruit number and yield.

Length and weight of the fruit
The length of the fruits of okra were the highest in T10 (15.21±0.11 cm) and the lowest in control (13.37±0.09 cm) and differed significantly (p<0.05). Length of the fruits increased with increase in nutrient content of the briquettes. An increase in nitrogen content could enhance various biological processes in plants [40]. Weight of the fruits obtained by using organic briquette as a nutrient source was the highest for T10 (15.35±0.18 gm) and the lowest for control (12.07±0.15 gm), with T10 differing significantly (p<0.05) from all other treatments.
Weight of the fruits increased with increasing nutrient content of the organic briquettes.
Nitrogen level in the soil was positively related to fruit weight [41], which could be due to the increased fruit weight in treatments compared to control. The increased nitrogen, phosphorus and potassium content of the growth medium due to the addition of briquettes, increased the transfer and assimilation of photosynthates in plants, which consequently accelerated the formation of greater number of large sized fruits with greater number of seeds per fruit, leading to increased fruit weight [35].

Total fruit yield per plant
Fruit yield was an important attribute of okra as it is directly related to economics of production. The fruit yield of okra in the current study was the highest in T10 (0.501±0.17 kg) and the lowest in control (0.160±0.16 kg). The fruits yield per plant is directly related to the number of fruits per plant and fruit weight [35] which underlines the reason for increased fruit yield in treatments compared to control. The increased fruit yield could also be attributed due to the increase in nitrogen levels [41]. The addition of organic manure to the soil in adequate amounts produced all the primary and secondary nutrients requires for crop growth [42].
Briquettes as organic nutrient sources might have improved physical and biological properties of the soil, subsequently increasing nutrient supply to plants, resulting in higher yield [43].

Table II. Effect of different treatments of organic briquettes on height of plant (cm) at different stages of growth
Mean ± S.E of different treatments having different superscripts (lower case letters a-e within rows, upper case letter A-D within columns) differ significantly (p<0.05).

Nutrient release pattern of organic briquettes
The growth and yield of plants depends on the availability of nutrients in the medium.
Effect of different treatments of organic briquettes on available nitrogen, phosphorus and potassium at different stages of plant growth followed a similar pattern and are represented in Figure I, II and III respectively. The maximum available nutrient content was seen at four MAP and minimum was seen at one MAP with significant (p<0.05) increase noted between three MAP and four MAP. Delayed nitrogen release was noticed in composted pellets in comparison with ordinary composts [18]. Due to the prevalence of anaerobic conditions inside the pellet, nitrification increases which become more evident in larger pellets. Similarly, slower release of nitrogen into the medium was observed in the current study. Organic materials having wide C: N ratio and high lignin content favour nutrient immobilisation rather than mineralisation [44]. Coir pith had high carbon: nitrogen ratio and lignin content [29]. The organic briquettes used in the study had a wide C: N ratio and had coir pith added in high amounts which could be the reason for slower nitrogen release during first two MAP.
In a study of application of bovine blood-rumen digesta mixture as a supplement for tomato cultivation, [27] also observed lower release of nitrogen at two weeks after planting compared to that of four weeks after planting. Similarly, maximum available nitrogen, phosphorus and potassium content in the soil were noticed after 45 days of application, when bovine digesta was added to the soil in raw form [45].
At all stages of growth, the lowest available nutrient content was noticed in T5 and the highest values were noted in T10. The available potassium and phosphorus contents in the soil increased with increase in rate of application of the organic fertilizer used [16,24]. The treatment T10 had more nutrient content compared to other treatments which might have contributed to the increased nutrient availability at different stages of growth. A combination of organic manures positively influenced the available NPK content of media which might be due to the added supply of nutrients [46]. The availability of nutrients in soil was markedly higher in treatment where blood and rumen contents were added in the ratio 3:1 compared to the treatments where blood and rumen contents are added in 2:1 and 1:1 ratio [16]. The nutrient release study indicated that the release pattern showed an increasing trend upto 4 MAP which coincides with maximum vegetative growth of okra.

CONCLUSION
The current study has revealed that compact organic briquettes developed using preprocessed rumen content-blood and coir pith have great potential to be used as a plant growth supplement in pot culture or grow bag cultivation of vegetables. The best desirable plant growth and fruit yield parameters were observed, when organic briquettes having 20% RB100 and 80% coir pith (T10) were utilised as an organic nutrient source. Three such briquettes were applied into each pot or grow bag of okra.
The availability of nutrient in the medium was relatively low in the initial phase of crop growth. However, at four MAP, higher levels of nutrients were available in the medium which resulted in better plant growth and fruit yield. The organic briquettes are an effective means for nutrient supply in pot culture especially in terrace garden for vegetables. The importance of developing briquettes from slaughterhouse wastes is underscored by the fact that, these two waste materials contribute greatest towards the environmental impact of service slaughterhouses and the consequent public dissent in Kerala. Large volume, cost effective equipment like solar driers can be utilised for preprocessing of rumen contents and blood. In smaller slaughterhouses, the dried rumen content can be directly used to receive blood from the bleeding animal in prescribed proportions, which eliminates the problem of run off of blood into the drainage and to the outside of the slaughterhouse. The development of organic briquettes is a viable option in the present context of lack of practical ways and means for waste disposal and organic agriculture in Kerala. The scope for further refinement of the work includes development of suitable methods of pre-processing of raw materials to improve the release of nutrients in the medium, analysis of briquettes for micro nutrients and suitable amendments if needed, enrichment with other organic materials to improve release efficiency of nutrients and the evaluating the effect of briquettes on the growth and yield parameters of other crops before field application.