Cervical cancer is one of the most common malignancies in women all over the world. Among the risk factors, the infection of HPV, especially the high risk type HPV virus, is the main cause of cervical cancer, and with the longer of carcinogenic infection lasting, showing the greater of risk [19, 20]. Most women with early-stage cervical tumors can be cured. Although benefit from the development of early detection and diagnostic technique, cervical cancer having a good prognosis, there are still many patients appeared tissues infiltration and metastasis and the morbidity and mortality of this cancer are still increasing in the developing countries . Thus, the accurate bio-markers are meaningful for the diagnosis and prognosis of cervical cancer.
To date, molecular markers have been identified to be involved in the tumorigenesis, development and progression of cancers, including cervical cancer . For instance, Azizmohammadi S et al. demonstrated that miRNA-145 and miR-9 were both up-regulated in cervical cancer and may be as potential prognostic markers in patients suffering from cervical cancer . SHI et al. suggested that secreted protein acidic and rich in cysteine (SPARC) may be a potential therapeutic option for cervical cancer patients . Liliana Alvarado-Ruiz et al. found that normal cervical cancer from women without cervical lesions expression HOXA9 but controlling HOXA9 expression appears to be a necessary step during cervical cancer development . These data suggested the crucial roles of cancer related molecules in cervical cancers. In the present study, we aimed at to identify a novel accurate diagnostic biomarker for patients with cervical cancer.
Upregulation of miR-221 has been reported in various cancers. Yilmaz SS et al. showed that miR-221 was upregulated in larynx cancer plasma samples and plasma miR-221 may be a potential diagnostic/prognostic marker in larynx cancer . Eissa S et al. indicated that the relative level of miR-221 expression in breast cancer tissues was higher than that in noncancerous tissues and it may be a potential biomarker and molecular therapeutic target for breast cancer . Yang et al. revealed that miR-221 played a crucial role in the occurrence and the progression of human osteosarcoma and may function as a promising marker for screening individuals with osteosarcoma . In study of Li et al., serum miR-221 expression levels has been indicated that significantly higher in patients with cutaneous malignant melanoma (CMM) and it has prognostic value in CMM patients . Previous study has found the aberrant expression of miR-221 in the progression of cervical cancer . But clinical diagnostic value of miR-221 in cervical cancer has not been investigated. In the present study, we sought to assess serum miR-221 expression pattern as well as its diagnostic role in patients with cervical cancer.
In this study, we measured the expression of miR-221 and the association between miR-221 with clinicopathological features in cervical cancer patients. The result of qRT-PCR revealed the expression of miR-221 in cervical cancer was increased compared with healthy control, which suggests that miR-221 may therefore function as a tumor oncogene. The elevated expression of miR-221 was correlated with lymph node metastasis and FIGO stage, which suggested that miR-221 was involved in the development of cervical cancer. Furthermore, the diagnostic value of miR-221 has also been investigated in our study using ROC curve analysis. The high AUC value, sensitivity and specificity values suggested that miR-221 may be a valuable diagnosis biomarker for cervical cancer detection.