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Research article
Jörg-Peter Schnitzler
Helmholtz Zentrum Munchen Deutsches Forschungszentrum fur Umwelt und Gesundheit
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9825-867X
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This work is licensed under a CC BY 4.0 License. Read Full License
Background
Tansy plants (Tanacetum vulgare L.) are known for their high intraspecific chemical variation, especially of volatile organic compounds (VOC) from the terpenoid compound group. These VOCs are closely involved in plant-insect interactions and, when profiled, can be used to classify plants into groups known as chemotypes. Tansy chemotypes have been shown to influence plant-aphid interactions, however, to date no information is available on the response of different tansy chemotypes to simultaneous herbivory by more than one insect species.
Results
Using a multi-cuvette system, we investigated the responses of five tansy chemotypes to feeding by sucking and/or chewing herbivores (aphids and caterpillars; Metopeurum fuscoviride Stroyan and Spodoptera littoralis Boisduval). Herbivory by caterpillars following aphid infestation led to a plant chemotype-specific change in the patterns of terpenoids stored in trichome hairs and in VOC emissions. The transcriptomic analysis of a plant chemotype represents the first de novo assembly of a transcriptome in tansy and demonstrates priming effects of aphids on a subsequent herbivory. Overall, we show that the five chemotypes do not react in the same way to the two herbivores. As expected, we found that caterpillar feeding increased VOC emissions, however, a priori aphid infestation only led to a further increase in VOC emissions for some chemotypes.
Conclusions
We were able to show that different chemotypes respond to the double herbivore attack in different ways, and that pre-treatment with aphids had a priming effect on plants when they were subsequently exposed to a chewing herbivore. If neighbouring chemotypes in a field population react differently to herbivory/dual herbivory, this could possibly have effects from the individual level to the group level. Individuals of some chemotypes may respond more efficiently to herbivory stress than others, and in a group environment these "louder" chemotypes may affect the local insect community, including the natural enemies of herbivores, and other neighbouring plants.
Keywords
Volatilome, Chemotypes, Herbivore
Figure 1
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Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- TableS1.tif
Table S1: Compounds identified in hexane and SBSE extraction analysis methods.
- TablesS24datadepictedinFigure235.xlsx
Table S2: Mean concentration of compounds of each plant chemotype found in hexane extractions at sampling days 4 and 7. Table S3: Mean VOC emission rates of each plant chemotype at sampling days 1-4 and 4-7. Table S4: Mean VOC emission rates of representative masses (m/z) measured using PTR-ToF-MS of different each plant chemotype at sampling days 1-4 and 4-7.
- TableS5allDEGs.xlsx
Table S5: List of differentially expressed genes
- TableS6defenceresponseDEGs.xlsx
Table S6: List of differentially expressed genes associated with defence responses
- FigureS1.tif
Figure S1: Schematic of cuvette platform and experimental setup.
- FigureS2.tif
Figure S2: Two-tailed t-test analysis of summed concentrations of all VOCS (measured in hexane extracts) across chemotypes per treatment. A significant difference was found between treatment groups B and N (t-value = -3.659, df = 4, P = 0.022). N: no aphid, no caterpillar, leaf material harvested on day 4; A: aphid, no caterpillar, leaf material harvested on day 7; C: no aphid, caterpillar, leaf material harvested on day 4; B: both aphid and caterpillar, leaf material harvested on day 7.
- FigureS3.tif
Figure S3: Visual representation of treatment groups for transcriptome analysis.
- FigureS4.tif
Figure S4: Phylogenetic tree of TPS genes obtained from tansy and other related species. TPS subfamilies are coloured as follows, blue: green: TPS-a, TPS-b, yellow: TPS-f.
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On 12 Aug, 2020
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On 11 Aug, 2020
Reviewers invited
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See the published version of this preprint at BMC Plant Biology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Jörg-Peter Schnitzler
Helmholtz Zentrum Munchen Deutsches Forschungszentrum fur Umwelt und Gesundheit
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9825-867X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Plant Biology.
No community comments so far
Submission checks complete
On 21 Nov, 2020
Editorial decision: Accept
On 16 Nov, 2020
No comments provided
Editorial decision: Minor revision
On 28 Oct, 2020
Editor assigned
On 27 Oct, 2020
Submission checks complete
On 26 Oct, 2020
Editor invited
On 26 Oct, 2020
Version 2
Posted 22 Oct, 2020
No comments provided
Review #1 received
Received 24 Oct, 2020
Editorial decision: Minor revision
On 24 Oct, 2020
Reviewer #2 agreed
On 14 Oct, 2020
Review #2 received
Received 14 Oct, 2020
Editor assigned
On 13 Oct, 2020
Reviewers invited
Invitations sent on 13 Oct, 2020
Reviewer #1 agreed
On 13 Oct, 2020
Submission checks complete
On 12 Oct, 2020
Editor invited
On 12 Oct, 2020
No comments provided
Editorial decision: Major revision
On 07 Sep, 2020
Review #3 received
Received 06 Sep, 2020
Review #1 received
Received 03 Sep, 2020
Reviewer #3 agreed
On 14 Aug, 2020
Review #2 received
Received 13 Aug, 2020
Reviewer #2 agreed
On 12 Aug, 2020
Reviewer #1 agreed
On 11 Aug, 2020
Reviewers invited
Invitations sent on 10 Aug, 2020
Editor invited
On 29 Jul, 2020
Editor assigned
On 23 Jul, 2020
Submission checks complete
On 22 Jul, 2020
First submitted
On 21 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Plant Biology.
Background
Tansy plants (Tanacetum vulgare L.) are known for their high intraspecific chemical variation, especially of volatile organic compounds (VOC) from the terpenoid compound group. These VOCs are closely involved in plant-insect interactions and, when profiled, can be used to classify plants into groups known as chemotypes. Tansy chemotypes have been shown to influence plant-aphid interactions, however, to date no information is available on the response of different tansy chemotypes to simultaneous herbivory by more than one insect species.
Results
Using a multi-cuvette system, we investigated the responses of five tansy chemotypes to feeding by sucking and/or chewing herbivores (aphids and caterpillars; Metopeurum fuscoviride Stroyan and Spodoptera littoralis Boisduval). Herbivory by caterpillars following aphid infestation led to a plant chemotype-specific change in the patterns of terpenoids stored in trichome hairs and in VOC emissions. The transcriptomic analysis of a plant chemotype represents the first de novo assembly of a transcriptome in tansy and demonstrates priming effects of aphids on a subsequent herbivory. Overall, we show that the five chemotypes do not react in the same way to the two herbivores. As expected, we found that caterpillar feeding increased VOC emissions, however, a priori aphid infestation only led to a further increase in VOC emissions for some chemotypes.
Conclusions
We were able to show that different chemotypes respond to the double herbivore attack in different ways, and that pre-treatment with aphids had a priming effect on plants when they were subsequently exposed to a chewing herbivore. If neighbouring chemotypes in a field population react differently to herbivory/dual herbivory, this could possibly have effects from the individual level to the group level. Individuals of some chemotypes may respond more efficiently to herbivory stress than others, and in a group environment these "louder" chemotypes may affect the local insect community, including the natural enemies of herbivores, and other neighbouring plants.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- TableS1.tif
Table S1: Compounds identified in hexane and SBSE extraction analysis methods.
- TablesS24datadepictedinFigure235.xlsx
Table S2: Mean concentration of compounds of each plant chemotype found in hexane extractions at sampling days 4 and 7. Table S3: Mean VOC emission rates of each plant chemotype at sampling days 1-4 and 4-7. Table S4: Mean VOC emission rates of representative masses (m/z) measured using PTR-ToF-MS of different each plant chemotype at sampling days 1-4 and 4-7.
- TableS5allDEGs.xlsx
Table S5: List of differentially expressed genes
- TableS6defenceresponseDEGs.xlsx
Table S6: List of differentially expressed genes associated with defence responses
- FigureS1.tif
Figure S1: Schematic of cuvette platform and experimental setup.
- FigureS2.tif
Figure S2: Two-tailed t-test analysis of summed concentrations of all VOCS (measured in hexane extracts) across chemotypes per treatment. A significant difference was found between treatment groups B and N (t-value = -3.659, df = 4, P = 0.022). N: no aphid, no caterpillar, leaf material harvested on day 4; A: aphid, no caterpillar, leaf material harvested on day 7; C: no aphid, caterpillar, leaf material harvested on day 4; B: both aphid and caterpillar, leaf material harvested on day 7.
- FigureS3.tif
Figure S3: Visual representation of treatment groups for transcriptome analysis.
- FigureS4.tif
Figure S4: Phylogenetic tree of TPS genes obtained from tansy and other related species. TPS subfamilies are coloured as follows, blue: green: TPS-a, TPS-b, yellow: TPS-f.
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