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Research Article
Wen Sun
Key Laboratory of Degraded and Unused Land Consolidation Engineering,
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Using samples collected in Shahe Reservoir in the upper North Canal in China, this research analyzes the structure of a microorganism group in sediment and the gene expression levels of two typical pathogenic bacteria (Escherichia coli and Enterococcus), and their relationship with environmental factors including total nitrogen (TN) and total phosphorus (TP). The study of samples collected from the surface (0–20 cm) and sediment cores shows that the absolute gene expression level of E. coli in in horizontal distribution in the sediment is higher than the relative gene expression level in the downstream of the reservoir and contaminated area. In vertical distribution, the absolute gene expression level of the two pathogenic bacteria in the sediment tends to decrease with increasing depth, although the relative gene expression level has its highest value at 10–30 cm depth. The relative gene expression level of the two pathogenic bacteria is much greater in the sediment of Shahe Reservoir with the structure of horizontal groups including Clortridium sensu stricto, unclassified Anaeroineaceae, and Povalibacter, while Anaeroineaceae is much more abundant in the group structure of the vertical distribution. Pearson correlation analysis suggests positive correlation in horizontal distribution for E. coli and TN and TP (P < 0.05) and for Enterococcus and TP (P < 0.05). The results clearly show that the amount of pathogenic bacteria in the sediment in Shahe Reservoir is most likely due to water eutrophication.
Keywords
pathogen, sediment, nutrient, correlation
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No competing interests reported.
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CURRENT STATUS: Under Review
Version 1
Posted 12 May, 2021
No community comments so far
Editorial decision: Major revision
On 26 Sep, 2021
Reviews received
Received 24 Sep, 2021
Reviewers agreed
On 06 Jun, 2021
Reviews received
Received 26 May, 2021
Reviewers agreed
On 24 May, 2021
Reviewers invited
Invitations sent on 12 May, 2021
Editor assigned
On 12 May, 2021
Editor invited
On 11 May, 2021
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On 10 May, 2021
First submitted
On 29 Apr, 2021
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Wen Sun
Key Laboratory of Degraded and Unused Land Consolidation Engineering,
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 12 May, 2021
No community comments so far
Editorial decision: Major revision
On 26 Sep, 2021
Reviews received
Received 24 Sep, 2021
Reviewers agreed
On 06 Jun, 2021
Reviews received
Received 26 May, 2021
Reviewers agreed
On 24 May, 2021
Reviewers invited
Invitations sent on 12 May, 2021
Editor assigned
On 12 May, 2021
Editor invited
On 11 May, 2021
Submission checks complete
On 10 May, 2021
First submitted
On 29 Apr, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Using samples collected in Shahe Reservoir in the upper North Canal in China, this research analyzes the structure of a microorganism group in sediment and the gene expression levels of two typical pathogenic bacteria (Escherichia coli and Enterococcus), and their relationship with environmental factors including total nitrogen (TN) and total phosphorus (TP). The study of samples collected from the surface (0–20 cm) and sediment cores shows that the absolute gene expression level of E. coli in in horizontal distribution in the sediment is higher than the relative gene expression level in the downstream of the reservoir and contaminated area. In vertical distribution, the absolute gene expression level of the two pathogenic bacteria in the sediment tends to decrease with increasing depth, although the relative gene expression level has its highest value at 10–30 cm depth. The relative gene expression level of the two pathogenic bacteria is much greater in the sediment of Shahe Reservoir with the structure of horizontal groups including Clortridium sensu stricto, unclassified Anaeroineaceae, and Povalibacter, while Anaeroineaceae is much more abundant in the group structure of the vertical distribution. Pearson correlation analysis suggests positive correlation in horizontal distribution for E. coli and TN and TP (P < 0.05) and for Enterococcus and TP (P < 0.05). The results clearly show that the amount of pathogenic bacteria in the sediment in Shahe Reservoir is most likely due to water eutrophication.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
No competing interests reported.
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