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Research
Vincent Odhiambo Nyasembe
International Centre for Insect Physiology and Ecology
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8018-5687
License:
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Background: In a recent study using DNA barcoding, we identified the plants fed upon by four Afro-tropical mosquito species that vector dengue, malaria, and Rift Valley fever. Herein, we have expanded on this study by investigating the role of three of the plants Pithecellobium dulce (Fabaceae), Leonotis nepetifolia (Lamiaceae), and Opuntia ficus-indica (Cactaceae) on survival, fecundity, and egg viability of the dengue vector Aedes aegypti.
Methods: We tested these effects using females that received a) an initial three rations of bloodmeal, and b) received no bloodmeal at all. Two controls were included; age-matched females fed on glucose solution with or without initial bloodmeal, and those fed exclusively on bloodmeal. Data was collected daily over a 30-day period. The amino acid content of Ae. aegypti guts and the amino acid content of their respective diets was detected by coupled liquid chromatography – mass spectrometry.
Results: Females fed on P. dulce and exclusive bloodmeal had a shorter survival than those fed on glucose. On the other hand, females fed on L. nepetifolia survived longer than those fed exclusively on bloodmeal, whereas those fed on O. ficus-indica had the shortest survival time. With initial bloodmeal, females fed on L. nepetifolia laid 1.6-fold more eggs while those fed on the other diets laid fewer eggs, compared to those fed exclusively on bloodmeal. Hatching rates of the eggs laid varied with the diet. Mass spectroscopic analysis of gut contents of mosquitoes exposed to the different diets showed qualitative and quantitative differences in their amino acid levels.
Conclusion: Our findings highlight the central role of plant nutrients in the reproductive fitness of dengue vectors which may impact their disease transmission potential.
Keywords
Plant nectar, plant sap, survival, fecundity, hatching-rates, amino acids, dengue
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CURRENT STATUS: Published
View the published article at Parasites & Vectors.
Version 3
Posted 11 Nov, 2020
No community comments so far
Editorial decision: Accept
On 02 Dec, 2020
Review #1 received
Received 14 Nov, 2020
Reviewer #2 agreed
On 10 Nov, 2020
Reviewer #1 agreed
On 08 Nov, 2020
Reviewers invited
Invitations sent on 07 Nov, 2020
Editor invited
On 06 Nov, 2020
Editor assigned
On 06 Nov, 2020
Submission checks complete
On 06 Nov, 2020
No comments provided
Editorial decision: Minor Revision
On 27 Oct, 2020
Review #3 received
Received 21 Oct, 2020
Review #2 received
Received 18 Oct, 2020
Review #1 received
Received 16 Oct, 2020
Reviewer #3 agreed
On 09 Oct, 2020
Reviewer #1 agreed
On 07 Oct, 2020
Reviewer #2 agreed
On 07 Oct, 2020
Reviewers invited
Invitations sent on 06 Oct, 2020
Editor invited
On 01 Oct, 2020
Editor assigned
On 01 Oct, 2020
Submission checks complete
On 30 Sep, 2020
No comments provided
Editorial decision: Major Revision
On 27 Aug, 2020
Review #3 received
Received 19 Aug, 2020
Review #1 received
Received 09 Aug, 2020
Review #2 received
Received 06 Aug, 2020
Reviewer #2 agreed
On 27 Jul, 2020
Reviewer #3 agreed
On 27 Jul, 2020
Reviewer #1 agreed
On 26 Jul, 2020
Reviewers invited
Invitations sent on 25 Jul, 2020
Editor invited
On 23 Jul, 2020
Editor assigned
On 23 Jul, 2020
First submitted
On 22 Jul, 2020
Submission checks complete
On 22 Jul, 2020
Metrics
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PDF Downloads: ...
HTML Views: ...
Subject Areas
Parasitology
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A new preprint design is coming!
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See the published version of this preprint at Parasites & Vectors.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Vincent Odhiambo Nyasembe
International Centre for Insect Physiology and Ecology
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8018-5687
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Parasites & Vectors.
Version 3
Posted 11 Nov, 2020
No community comments so far
Editorial decision: Accept
On 02 Dec, 2020
Review #1 received
Received 14 Nov, 2020
Reviewer #2 agreed
On 10 Nov, 2020
Reviewer #1 agreed
On 08 Nov, 2020
Reviewers invited
Invitations sent on 07 Nov, 2020
Editor invited
On 06 Nov, 2020
Editor assigned
On 06 Nov, 2020
Submission checks complete
On 06 Nov, 2020
No comments provided
Editorial decision: Minor Revision
On 27 Oct, 2020
Review #3 received
Received 21 Oct, 2020
Review #2 received
Received 18 Oct, 2020
Review #1 received
Received 16 Oct, 2020
Reviewer #3 agreed
On 09 Oct, 2020
Reviewer #1 agreed
On 07 Oct, 2020
Reviewer #2 agreed
On 07 Oct, 2020
Reviewers invited
Invitations sent on 06 Oct, 2020
Editor invited
On 01 Oct, 2020
Editor assigned
On 01 Oct, 2020
Submission checks complete
On 30 Sep, 2020
No comments provided
Editorial decision: Major Revision
On 27 Aug, 2020
Review #3 received
Received 19 Aug, 2020
Review #1 received
Received 09 Aug, 2020
Review #2 received
Received 06 Aug, 2020
Reviewer #2 agreed
On 27 Jul, 2020
Reviewer #3 agreed
On 27 Jul, 2020
Reviewer #1 agreed
On 26 Jul, 2020
Reviewers invited
Invitations sent on 25 Jul, 2020
Editor invited
On 23 Jul, 2020
Editor assigned
On 23 Jul, 2020
First submitted
On 22 Jul, 2020
Submission checks complete
On 22 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Parasitology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Parasites & Vectors.
Background: In a recent study using DNA barcoding, we identified the plants fed upon by four Afro-tropical mosquito species that vector dengue, malaria, and Rift Valley fever. Herein, we have expanded on this study by investigating the role of three of the plants Pithecellobium dulce (Fabaceae), Leonotis nepetifolia (Lamiaceae), and Opuntia ficus-indica (Cactaceae) on survival, fecundity, and egg viability of the dengue vector Aedes aegypti.
Methods: We tested these effects using females that received a) an initial three rations of bloodmeal, and b) received no bloodmeal at all. Two controls were included; age-matched females fed on glucose solution with or without initial bloodmeal, and those fed exclusively on bloodmeal. Data was collected daily over a 30-day period. The amino acid content of Ae. aegypti guts and the amino acid content of their respective diets was detected by coupled liquid chromatography – mass spectrometry.
Results: Females fed on P. dulce and exclusive bloodmeal had a shorter survival than those fed on glucose. On the other hand, females fed on L. nepetifolia survived longer than those fed exclusively on bloodmeal, whereas those fed on O. ficus-indica had the shortest survival time. With initial bloodmeal, females fed on L. nepetifolia laid 1.6-fold more eggs while those fed on the other diets laid fewer eggs, compared to those fed exclusively on bloodmeal. Hatching rates of the eggs laid varied with the diet. Mass spectroscopic analysis of gut contents of mosquitoes exposed to the different diets showed qualitative and quantitative differences in their amino acid levels.
Conclusion: Our findings highlight the central role of plant nutrients in the reproductive fitness of dengue vectors which may impact their disease transmission potential.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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