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Research article
jin xu
Shandong Provincial Hospital
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
As the incidence of secretory osteoporosis increases, bone loss and osteoporosis and their relationships with thyroid-stimulating hormone (TSH) have received increased attention. In this study, the role of TSH in bone metabolism and the underlying possible mechanisms were investigated.
Methods
We analyzed serum triiodothyronine (FT3), tetraiodothyronine (FT4), TSH and bone mineral density (BMD) levels of 114 men with normal thyroid function. In addition, osteoblasts from rat calvarial samples were treated with different doses of TSH for different times at each time point. The related gene and protein expression levels were investigated.
Results
Comparing the BMD between the high-level and low-level serum TSH group showed that TSH serum concentrations were positively correlated with BMD. TSH at concentrations of 10 mU/mL and 100 mU/mL significantly increased the mRNA levels of ALP, COI1 and Runx2 compared with those of the control (P < 0.05, P < 0.01). BMP2 activity was enhanced both with increased TSH concentration and with increased time. The protein levels of Runx2 and osterix were increased in a dose-dependent manner.
Conclusions
The circulating concentrations of TSH and BMD were positively correlated with normal thyroid function in males. TSH promoted osteoblast proliferation and differentiation in rat primary osteoblasts.
Keywords
osteoporosis, TSH, BMD, osteoblast
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CURRENT STATUS: Published
View the published article at BMC Endocrine Disorders.
Version 1
Posted 07 Oct, 2020
No community comments so far
Editorial decision: Revise before sending to peer reviewers
On 12 Oct, 2020
Editor assigned
On 28 Sep, 2020
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On 27 Sep, 2020
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On 27 Sep, 2020
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Subject Areas
Endocrinology & Metabolism
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Endocrine Disorders.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
jin xu
Shandong Provincial Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Endocrine Disorders.
Version 1
Posted 07 Oct, 2020
No community comments so far
Editorial decision: Revise before sending to peer reviewers
On 12 Oct, 2020
Editor assigned
On 28 Sep, 2020
Submission checks complete
On 27 Sep, 2020
Editor invited
On 27 Sep, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Endocrinology & Metabolism
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Endocrine Disorders.
Background
As the incidence of secretory osteoporosis increases, bone loss and osteoporosis and their relationships with thyroid-stimulating hormone (TSH) have received increased attention. In this study, the role of TSH in bone metabolism and the underlying possible mechanisms were investigated.
Methods
We analyzed serum triiodothyronine (FT3), tetraiodothyronine (FT4), TSH and bone mineral density (BMD) levels of 114 men with normal thyroid function. In addition, osteoblasts from rat calvarial samples were treated with different doses of TSH for different times at each time point. The related gene and protein expression levels were investigated.
Results
Comparing the BMD between the high-level and low-level serum TSH group showed that TSH serum concentrations were positively correlated with BMD. TSH at concentrations of 10 mU/mL and 100 mU/mL significantly increased the mRNA levels of ALP, COI1 and Runx2 compared with those of the control (P < 0.05, P < 0.01). BMP2 activity was enhanced both with increased TSH concentration and with increased time. The protein levels of Runx2 and osterix were increased in a dose-dependent manner.
Conclusions
The circulating concentrations of TSH and BMD were positively correlated with normal thyroid function in males. TSH promoted osteoblast proliferation and differentiation in rat primary osteoblasts.
Figure 1
Figure 2
Figure 3
Figure 4
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