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Research Article
Bo Huang
Anhui Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6032-7396
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This work is licensed under a CC BY 4.0 License. Read Full License
The entomopathogenic fungus Beauveria bassiana is used worldwide for its biological control. Seven dsRNA segments were detected from a single Beauveria bassiana strain RCEF 1446. High-throughput sequencing indicated the presence of three mycoviruses in the infected sample. Two known mycoviruses were identified as Beauveria bassiana Victorivirus 1 and Beauveria bassiana polymycovirus 1, and the novel mycovirus was designated as Beauveria bassiana bipartite mycovirus 1 (BbBV1). The complete sequence of the BbBV1 is described here. The mycovirus contains two dsRNA segments. The first dsRNA is 2,026 bp in length and encoding a RNA-dependent RNA polymerase (RdRp) (68.54 kDa), while the second segment encoding a hypothetical protein (35.55 kDa) of unknown function, was 1,810 bp in length. Moreover, the RdRp protein sequences showed the highest identity of 62.89% to Corynespora cassiicola bipartite mycovirus 1. Phylogenetic analysis of the RdRp reveals that the virus represents a distinct lineage of unassigned dsRNA mycoviruses infecting fungi.
Keywords
entomopathogenic fungus, mycoviruses, Beauveria bassiana polymycovirus, RdRp
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementaryData1.txt
- fig.S1.jpg
Fig. S1 RT-PCR confirmation of the three mycoviruses infecting Beauveria bassiana M, DNA marker; dsRNA1 identified as Beauveria bassiana Victorivirus 1; dsRNA2, dsRNA 3, dsRNA 5, and dsRNA 7 consisting of Beauveria bassiana polymycovirus 1; dsRNA4 and dsRNA6 corresponding to BbBV1.
- BbBV1Nucleotidesequences.txt
- BbBV1Proteinsequences.txt
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CURRENT STATUS: Under Review
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Posted 11 May, 2021
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Received 06 May, 2021
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On 06 May, 2021
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This preprint is under consideration at Archives of Virology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Bo Huang
Anhui Agricultural University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6032-7396
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 11 May, 2021
No community comments so far
Reviews received
Received 06 May, 2021
Reviewers invited
Invitations sent on 06 May, 2021
Editor assigned
On 06 May, 2021
First submitted
On 06 May, 2021
Editorial decision: Major Revision - English Corrections
On 04 May, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Virology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The entomopathogenic fungus Beauveria bassiana is used worldwide for its biological control. Seven dsRNA segments were detected from a single Beauveria bassiana strain RCEF 1446. High-throughput sequencing indicated the presence of three mycoviruses in the infected sample. Two known mycoviruses were identified as Beauveria bassiana Victorivirus 1 and Beauveria bassiana polymycovirus 1, and the novel mycovirus was designated as Beauveria bassiana bipartite mycovirus 1 (BbBV1). The complete sequence of the BbBV1 is described here. The mycovirus contains two dsRNA segments. The first dsRNA is 2,026 bp in length and encoding a RNA-dependent RNA polymerase (RdRp) (68.54 kDa), while the second segment encoding a hypothetical protein (35.55 kDa) of unknown function, was 1,810 bp in length. Moreover, the RdRp protein sequences showed the highest identity of 62.89% to Corynespora cassiicola bipartite mycovirus 1. Phylogenetic analysis of the RdRp reveals that the virus represents a distinct lineage of unassigned dsRNA mycoviruses infecting fungi.
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementaryData1.txt
- fig.S1.jpg
Fig. S1 RT-PCR confirmation of the three mycoviruses infecting Beauveria bassiana M, DNA marker; dsRNA1 identified as Beauveria bassiana Victorivirus 1; dsRNA2, dsRNA 3, dsRNA 5, and dsRNA 7 consisting of Beauveria bassiana polymycovirus 1; dsRNA4 and dsRNA6 corresponding to BbBV1.
- BbBV1Nucleotidesequences.txt
- BbBV1Proteinsequences.txt
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