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Wenquan Wang
Corresponding Author
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Background: It is well known that cassava (Manihot esculenta Crantz) high efficient accumulates starch in its storage root underground, but how the photosynthates transported from leaves to the phloem, especially unloaded into the parenchymal cell of storage root remains unclear.
Methods: Here, we investigated the phloem unloading pattern of sucrose and its impact to the development of storage root in cassava through microstructural and physiological analyses, trace of carboxyfluorescein (CF) and Isotope C14. Identification of expression profiling of the genes involved in symplasmic and apoplasmic transport and their correlation with storage root yield in population by enzymatic activity, western blotting analysis and Transcriptome Sequencing.
Results: The carbohydrates are transported mainly as form of sucrose with above 54.6% measured with stem phloem instantly. The sucrose was predominantly symplasmic unloading from phloem into storage root, but there was a shift from apoplasmic to symplasmic unloading accompanied by the onset of root swelling. Microstructure statistics revealed the plasmodesmata enriched among sieve, companion and parenchyma cells in the developing storage root of cultivated variety but not in a wild ancestor. Tracing test with CF verified exist of coplastid channel and [14C]Suc demonstrated that the sucrose marked could rapid diffusion into root parenchyma cells from phloem cells. The higher expression of genes and proteins for SuSys in storage root at middle and late stage but not in early stage of storage root, primary fibrous root and second fibrous root, and the inversely expressed pattern of SUTs, CWI and SAI in these corresponding organs supported that there are a symplasmic sucrose unloading pathway. The transcriptomic pattern of genes involved in symplasmic unloading and their significantly positive correction with starch yielding in population level confirmed it is vital important that the symplasmic sucrose transport in developing storage root of cassava.
Conclusions: In the study, we uncovered a predominantly symplasmic phloem unloading pattern of sucrose in the storage root of cassava. And this advantage is essential to efficient starch accumulation for sucrose transports between a high yield variety to low yield wild ancestor.
Keywords
Cassava, Carbohydrates, Storage root, Symplasmic unloading, Starch yielding
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CURRENT STATUS: Under Review
The most recent version of this article is available here.
No community comments so far
Editorial decision: Major revision
On 08 Feb, 2021
Review #2 received
Received 26 Jan, 2021
Review #1 received
Received 18 Jan, 2021
Reviewer #2 agreed
On 06 Jan, 2021
Reviewer #1 agreed
On 06 Jan, 2021
Reviewers invited
Invitations sent on 06 Dec, 2020
Editor assigned
On 29 Nov, 2020
Submission checks complete
On 29 Nov, 2020
Editor invited
On 29 Nov, 2020
No comments provided
Editor assigned
On 08 Nov, 2020
Submission checks complete
On 08 Nov, 2020
Editor invited
On 08 Nov, 2020
Version 1
Posted 03 Aug, 2020
No comments provided
Editorial decision: Revise before sending to peer reviewers
On 28 Oct, 2020
Editor invited
On 31 Jul, 2020
Editor assigned
On 26 Jul, 2020
Submission checks complete
On 25 Jul, 2020
First submitted
On 24 Jul, 2020
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This preprint is under consideration at BMC Plant Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Wenquan Wang
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
The most recent version of this article is available here.
No community comments so far
Editorial decision: Major revision
On 08 Feb, 2021
Review #2 received
Received 26 Jan, 2021
Review #1 received
Received 18 Jan, 2021
Reviewer #2 agreed
On 06 Jan, 2021
Reviewer #1 agreed
On 06 Jan, 2021
Reviewers invited
Invitations sent on 06 Dec, 2020
Editor assigned
On 29 Nov, 2020
Submission checks complete
On 29 Nov, 2020
Editor invited
On 29 Nov, 2020
No comments provided
Editor assigned
On 08 Nov, 2020
Submission checks complete
On 08 Nov, 2020
Editor invited
On 08 Nov, 2020
Version 1
Posted 03 Aug, 2020
No comments provided
Editorial decision: Revise before sending to peer reviewers
On 28 Oct, 2020
Editor invited
On 31 Jul, 2020
Editor assigned
On 26 Jul, 2020
Submission checks complete
On 25 Jul, 2020
First submitted
On 24 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The most recent version of this article is available here.
Background: It is well known that cassava (Manihot esculenta Crantz) high efficient accumulates starch in its storage root underground, but how the photosynthates transported from leaves to the phloem, especially unloaded into the parenchymal cell of storage root remains unclear.
Methods: Here, we investigated the phloem unloading pattern of sucrose and its impact to the development of storage root in cassava through microstructural and physiological analyses, trace of carboxyfluorescein (CF) and Isotope C14. Identification of expression profiling of the genes involved in symplasmic and apoplasmic transport and their correlation with storage root yield in population by enzymatic activity, western blotting analysis and Transcriptome Sequencing.
Results: The carbohydrates are transported mainly as form of sucrose with above 54.6% measured with stem phloem instantly. The sucrose was predominantly symplasmic unloading from phloem into storage root, but there was a shift from apoplasmic to symplasmic unloading accompanied by the onset of root swelling. Microstructure statistics revealed the plasmodesmata enriched among sieve, companion and parenchyma cells in the developing storage root of cultivated variety but not in a wild ancestor. Tracing test with CF verified exist of coplastid channel and [14C]Suc demonstrated that the sucrose marked could rapid diffusion into root parenchyma cells from phloem cells. The higher expression of genes and proteins for SuSys in storage root at middle and late stage but not in early stage of storage root, primary fibrous root and second fibrous root, and the inversely expressed pattern of SUTs, CWI and SAI in these corresponding organs supported that there are a symplasmic sucrose unloading pathway. The transcriptomic pattern of genes involved in symplasmic unloading and their significantly positive correction with starch yielding in population level confirmed it is vital important that the symplasmic sucrose transport in developing storage root of cassava.
Conclusions: In the study, we uncovered a predominantly symplasmic phloem unloading pattern of sucrose in the storage root of cassava. And this advantage is essential to efficient starch accumulation for sucrose transports between a high yield variety to low yield wild ancestor.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
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