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Shouhua Pan
Shaoxing People's Hospital
Corresponding Author
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Background: MicroRNA (miRNA)-340 is emerging as a critical regulator for the development and progression of various cancers, such as oral squamous cell carcinoma and prostate cancer. However, little was known about the role of miR-340 in bladder cancer.
Methods: Bladder cancer and adjacent non-tumor tissue samples were collected from thirty patients undergoing surgical resection at Shaoxing People's Hospital. The expression of miR-340 and Glut-1 were studied via real-time quantitative PCR (RT-qPCR) or western blots. Bladder cancer T24 cells were transfected with miR-340 mimics to explore cell proliferation and apoptosis via MTT assay, flow cytometer, RT-qPCR and western blots.
Results: Compared with that of normal tissues, miR-340 expression was significantly lower, while both mRNA and protein expression of Glut-1 were higher in bladder cancer tissues. The miR-340 could negatively regulate Glut-1 expression in bladder cells. Moreover, bladder cell proliferation could be inhibited by miR-340 and the corresponding antitumor effect could partially reverse by the overexpression of Glut-1. In view of the complexity of gene networks, some other multiple pathways might also confer to miR-340 inducing bladder cell apoptosis, including downregulating PCNA, upregulating Bax and decreasing the phosphorylation levels of PI3K and AKT.
Conclusion: This work suggested an inverse correlation between miR-340 and glucose transporter-1 (Glut-1) expression in bladder cancer. miR-340/Glut-1 axis might be a potential and novel therapeutic target for the treatment of bladder cancer. More investigations need to further explore the applications of miR-340 in bladder cancer.
Keywords
microRNA-340, glucose transporter-1, bladder cancer, cell growth
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
- FigureS1.jpg
A) The mRNA of Glut-1 expression was determined by RT-qPCR in bladder carcinoma cells in transfected with pcDNA 3.1 or pcDNA 3.1-Glut-1. C) The protein level of Glut-1 in cells transfected with transfected with pcDNA 3.1 or pcDNA 3.1-Glut-1.
- FigureS1.jpg
A) The mRNA of Glut-1 expression was determined by RT-qPCR in bladder carcinoma cells in transfected with pcDNA 3.1 or pcDNA 3.1-Glut-1. C) The protein level of Glut-1 in cells transfected with transfected with pcDNA 3.1 or pcDNA 3.1-Glut-1.
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Shouhua Pan
Shaoxing People's Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 03 Aug, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: MicroRNA (miRNA)-340 is emerging as a critical regulator for the development and progression of various cancers, such as oral squamous cell carcinoma and prostate cancer. However, little was known about the role of miR-340 in bladder cancer.
Methods: Bladder cancer and adjacent non-tumor tissue samples were collected from thirty patients undergoing surgical resection at Shaoxing People's Hospital. The expression of miR-340 and Glut-1 were studied via real-time quantitative PCR (RT-qPCR) or western blots. Bladder cancer T24 cells were transfected with miR-340 mimics to explore cell proliferation and apoptosis via MTT assay, flow cytometer, RT-qPCR and western blots.
Results: Compared with that of normal tissues, miR-340 expression was significantly lower, while both mRNA and protein expression of Glut-1 were higher in bladder cancer tissues. The miR-340 could negatively regulate Glut-1 expression in bladder cells. Moreover, bladder cell proliferation could be inhibited by miR-340 and the corresponding antitumor effect could partially reverse by the overexpression of Glut-1. In view of the complexity of gene networks, some other multiple pathways might also confer to miR-340 inducing bladder cell apoptosis, including downregulating PCNA, upregulating Bax and decreasing the phosphorylation levels of PI3K and AKT.
Conclusion: This work suggested an inverse correlation between miR-340 and glucose transporter-1 (Glut-1) expression in bladder cancer. miR-340/Glut-1 axis might be a potential and novel therapeutic target for the treatment of bladder cancer. More investigations need to further explore the applications of miR-340 in bladder cancer.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
- FigureS1.jpg
A) The mRNA of Glut-1 expression was determined by RT-qPCR in bladder carcinoma cells in transfected with pcDNA 3.1 or pcDNA 3.1-Glut-1. C) The protein level of Glut-1 in cells transfected with transfected with pcDNA 3.1 or pcDNA 3.1-Glut-1.
- FigureS1.jpg
A) The mRNA of Glut-1 expression was determined by RT-qPCR in bladder carcinoma cells in transfected with pcDNA 3.1 or pcDNA 3.1-Glut-1. C) The protein level of Glut-1 in cells transfected with transfected with pcDNA 3.1 or pcDNA 3.1-Glut-1.
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