Naturally occurring plant based biostimulants can be used to improve crop productivity and quality as an eco-friendly approach. Moringa oleifera, known as the miracle tree, is a rich source of essential nutrients and beneficial compounds that can act as potent growth enhancer. The current work aimed to appraise whether moringa dried leaf extract (MDLE) could improve growth, physiology, seed nutritional and quality attributes of soybean. The designed study followed a completely randomized design (CRD) with factorial arrangement having four replications examining various MDLE concentrations [water spray as control, 0.5%, 1.0%, and 1.5% (w/v)] and growth stages (vegetative and reproductive) of soybean. The results revealed that foliar application of 1.5% MDLE during the reproductive stage of soybean showcased remarkable enhancements. Notably, seedling growth metrics, photosynthetic pigments, leaf area, gas exchange parameters, yield attributes, seed nutritional and quality attributes were significantly boosted. Overall, a noteworthy increase in seedling growth metrics (22%), carotenoids (8.33%), gas exchange attributes (18%), yield components (37%) and seed nutritional attributes (18%) was observed with 1.5% MDLE application during the reproductive stage. This underscores the potential of MDLE foliar application during reproductive stage in improving soybean growth, quality and seed nutritional contents.