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Research Article
PURVI PUROHIT
All India Institute of Medical Sciences Jodphur
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8559-2911
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The current study determined levels of inflammatory cytokines TNF-α, IL-6, IL-10 and immune-regulatory miR-155 and miR-187 expressions in chronic opiate abuse. Adults (n = 48), meeting the 5th Edition of the DSM criteria regarding opioid use disorder, and healthy controls (n = 46) were included in the study. Serum samples were analysed for inflammatory cytokines IL-10, IL-6, TNF-α using ELISA and PBMCs processed for miRNA expression using SybrGreen chemistry. Cases showed significantly raised IL-10 and TNF-α and reduced IL-6. Using RNU6 for normalization, dose-dependent corresponding upregulation of miR-155-5p and downregulation of miR-187-5p were evident at opiate dose > 1500 gm/day, with a corresponding increase of TNF-α and IL-10. MiR-155 showed a significant negative correlation with IL-6 and TNF-α; miR-187 showed a significant positive association with TNF-α at > 1000 g/day consumption. Therefore, increasing consumption of opium probably enhances inflammation leading to immunomodulation and aberrant expression of has-miR-155-5p and has-miR-187-5p in opiate abusers.
Keywords
MicroRNA, Opiate, IL-10, IL-6, TNF-α
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CURRENT STATUS: Under Review
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Posted 12 May, 2021
No community comments so far
Editorial decision: Reconsider with major revisions
On 11 Jun, 2021
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Received 10 May, 2021
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A new preprint design is coming!
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This preprint is under consideration at Inflammation. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
PURVI PUROHIT
All India Institute of Medical Sciences Jodphur
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8559-2911
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 12 May, 2021
No community comments so far
Editorial decision: Reconsider with major revisions
On 11 Jun, 2021
Reviews received
Received 10 May, 2021
Reviewers invited
Invitations sent on 10 May, 2021
Editor assigned
On 09 May, 2021
First submitted
On 01 May, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The current study determined levels of inflammatory cytokines TNF-α, IL-6, IL-10 and immune-regulatory miR-155 and miR-187 expressions in chronic opiate abuse. Adults (n = 48), meeting the 5th Edition of the DSM criteria regarding opioid use disorder, and healthy controls (n = 46) were included in the study. Serum samples were analysed for inflammatory cytokines IL-10, IL-6, TNF-α using ELISA and PBMCs processed for miRNA expression using SybrGreen chemistry. Cases showed significantly raised IL-10 and TNF-α and reduced IL-6. Using RNU6 for normalization, dose-dependent corresponding upregulation of miR-155-5p and downregulation of miR-187-5p were evident at opiate dose > 1500 gm/day, with a corresponding increase of TNF-α and IL-10. MiR-155 showed a significant negative correlation with IL-6 and TNF-α; miR-187 showed a significant positive association with TNF-α at > 1000 g/day consumption. Therefore, increasing consumption of opium probably enhances inflammation leading to immunomodulation and aberrant expression of has-miR-155-5p and has-miR-187-5p in opiate abusers.
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