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Research Article
Zheng-kun Zhang
Jilin Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8822-2107
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Beauveria bassiana, an entomopathogenic fungus, has a wide host range and is used for arthropod pest control worldwide. Here, we report the discovery and characterization of a novel double-stranded RNA (dsRNA) mycovirus Beauveria bassiana chrysovirus 2 (BbCV2), isolated from the B. bassiana from China. The genome of the virus was determined by metagenomic sequencing, RT-PCR, and RACE cloning comprises four dsRNA segments that are 3441bp, 2779bp, 2925bp, and 2688bp long, respectively, each of them contains a single ORF, the first one (ORF1) encoding a 1115-amino-acid-long protein (122.65 kDa) with a conserved RNA-dependent RNA polymerase (RdRp) motif, its sequences showed the highest identity of only 16.13% to that of the Beauveria bassiana chrysovirus 1. The second ORF (ORF-2) encoding a 807-amino-acid-long coat protein (CP) (88.77 kDa). The virus constitutes a new member of the chrysoviridea family from B. bassiana.
Keywords
double-stranded RNA virus, chrysovirus, chrysoviridea, Beauveria bassiana
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CURRENT STATUS: Under Review
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Posted 11 May, 2021
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Received 07 May, 2021
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This preprint is under consideration at Archives of Virology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Zheng-kun Zhang
Jilin Academy of Agricultural Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8822-2107
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 11 May, 2021
No community comments so far
Reviews received
Received 07 May, 2021
Reviewers invited
Invitations sent on 06 May, 2021
Editor assigned
On 06 May, 2021
Editorial decision: Major Revision
On 04 May, 2021
First submitted
On 01 May, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Virology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Beauveria bassiana, an entomopathogenic fungus, has a wide host range and is used for arthropod pest control worldwide. Here, we report the discovery and characterization of a novel double-stranded RNA (dsRNA) mycovirus Beauveria bassiana chrysovirus 2 (BbCV2), isolated from the B. bassiana from China. The genome of the virus was determined by metagenomic sequencing, RT-PCR, and RACE cloning comprises four dsRNA segments that are 3441bp, 2779bp, 2925bp, and 2688bp long, respectively, each of them contains a single ORF, the first one (ORF1) encoding a 1115-amino-acid-long protein (122.65 kDa) with a conserved RNA-dependent RNA polymerase (RdRp) motif, its sequences showed the highest identity of only 16.13% to that of the Beauveria bassiana chrysovirus 1. The second ORF (ORF-2) encoding a 807-amino-acid-long coat protein (CP) (88.77 kDa). The virus constitutes a new member of the chrysoviridea family from B. bassiana.
Figure 1
Figure 2
Figure 3
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