Membrane and lipid metabolism plays an important role in desiccation resistance in the yeast Saccharomyces cerevisiae

doi:10.21203/rs.3.rs-48916/v2

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Membrane and lipid metabolism plays an important role in desiccation resistance in the yeast Saccharomyces cerevisiae

https://doi.org/10.21203/rs.3.rs-48916/v2

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published 10 Nov, 2020

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Background

Anhydrobiotes, such as the yeast Saccharomyces cerevisiae, are capable of surviving almost total loss of water. Desiccation tolerance requires an interplay of multiple events, including preserving the protein function and membrane integrity, preventing and mitigating oxidative stress, maintaining certain level of energy required for cellular activities in the desiccated state. Many of these crucial processes can be controlled and modulated at the level of organelle morphology and dynamics. However, little is understood about what organelle perturbations manifest in desiccation-sensitive cells as a consequence of drying or how this differs from organelle biology in desiccation-tolerant organisms undergoing anhydrobiosis.

Results

In this study, electron and optical microscopy was used to examine the dynamic changes of yeast cells during the desiccation process. Dramatic structural changes were observed during the desiccation process, including the diminishing of vacuoles, decrease of lipid droplets, decrease in mitochondrial cristae and increase of ER membrane, which is likely caused by ER stress and unfolded protein response. The survival rate was significantly decreased in mutants that are defective in lipid droplet biosynthesis, or cells treated with cerulenin, an inhibitor of fatty acid synthesis.

Conclusion

Our study suggests that the metabolism of lipid droplets and membrane may play an important role in yeast desiccation tolerance by providing cells with energy and possibly metabolic water. Additionally, the decrease in mitochondrial cristae coupled with a decrease in lipid droplets is indicative of a cellular response to reduce the production of reactive oxygen species.

General Microbiology

Applied & Industrial Microbiology

anhydrobiosis

desiccation tolerance

endoplasmic reticulum

lipid droplets

lipid metabolism

mitochondria

vacuole

Water is essential for all life and required for the majority of the biological processes. At cellular level, water plays a pivotal role in proper protein folding and the assembly of phospholipids into biological membranes. Nevertheless, certain organisms called anhydrobiotes are capable of surviving extreme water loss for a long period of time. Anhydrobiotes are widespread, having been found in the plant, animal (such as tardigrades and nematodes), fungal (such as the budding yeast Saccharomyces cerevisiae and other non-conventional yeasts), and prokaryotic kingdoms of life [1]. Studies have shown that many anhydrobiotes possess large quantities of non-reducing sugars (mostly disaccharides, such as trehalose) [2], intrinsically disordered proteins (IDPs) [3-4] and heat shock proteins [5]. It is believed that these non-reducing sugars and IDPs help stabilize and preserve both membrane and protein structure and therefore mitigate the desiccation-induced stresses [6-9]. In budding yeast, dramatic structural changes during dehydration have been observed, including cell wall, cytoplasmic and nuclear membrane [10-12]. Some of the changes are caused by oxidative stress during dehydration [13], when the oxidation level increases significantly [14]. Multiple pathways have been evolved in yeast to mitigate the oxidative damages caused by desiccation [13].

The desiccation tolerance of anhydrobiotes is typically not innate, but acquired through some ‘low’ level stress that may or not be related to desiccation. For example, in plants acquisition of desiccation tolerance is triggered by dehydration and an increase in the plant hormone abscisic acid [6, 15]. In tardigrade and Saccharomyces cerevisiae, desiccation tolerance is induced by starvation [4, 16]. Dividing yeast cells growing in glucose have a minimum tolerance to desiccation [16]. Cells growing in ethanol-containing medium also has a decreased desiccation tolerance, possibly due to their high degree of unsaturated fatty acids [17]. Starvation stress leads to significant physiological and morphological changes in yeast, such as a reduced metabolic rate and thickened cell wall that is more resistant to enzymatic digestion and more thermotolerant [18]. Interestingly, a similar gene expression pattern to stationary yeast was identified in proliferating yeast that were treated with certain chemicals, heat shock or irradiation [19], suggesting that yeast may possess a universal response mechanism to different environmental stresses.

In plants, the desiccation process starts with moderate dehydration (initial loss of water) and precedes to severe dehydration (loss of almost all water). In the initial stage of dehydration, the cell volume decreases due to water loss, causing cytoplasmic components to become more crowded, which could cause protein denaturation and membrane fusion [6]. The protective disaccharides and other molecules accumulate and help forming a water shell around proteins and membrane to prevent proteins misfolding as well as phase transition of membranes. In the severe water loss stage, these disaccharides function as water substitutes to replace water molecules and preserve the protein and membrane structure [2,6]. The disaccharides could also be vitrified, especially at low water content, forming glassy matrix to avoid the membrane phase transition during dehydration [12, 20-22]. However, protein misfolding has been observed in desiccated yeast [5], suggesting that not all proteins, or membrane are protected. It is unknown whether ‘essential’ proteins and membrane structure (such as nuclear membrane) are preferentially protected.

During the desiccation process, intracellular water is still critical to maintain enzyme activities necessary to adapt to water loss. Enzyme activities were also observed in yeast during long-term desiccation (up to 180 days) when trehalose is degraded by trehalases [5]. Little is known about how trehalases remain active in this highly dehydrated state, suggesting that more mechanisms are involved in response to desiccation stress and desiccation tolerance. Not surprisingly, the necessity of several mechanisms to confer desiccation tolerance has also been reported in other systems [6].

In this study, transmission electron microscopy (TEM) was used to examine the ultrastructural changes of yeast during the desiccation process. Because stationary cells are more desiccation tolerant than the log-phase cells, we speculated that the morphological and physiological changes of stationary cells, in particular, the increased endoplasmic membrane, decrease in vacuoles and mitochondria and modulation of lipid droplet number may play an important role in desiccation tolerance of the yeast.

Desiccation causes significant structural changes

Desiccation causes cellular damages even in anhydrobiotes [11]. In budding yeast, studies have shown that desiccation causes changes of the cellular structure, such as cell wall, plasma membrane, nucleus and mitochondria (for reviews, see [10-12]). Here, transmission electron microscopy (TEM) was employed to examine and compare the ultrastructure of desiccated yeast cells in log- (desiccation-sensitive) and stationary- (desiccation tolerant) phases. Staining with potassium permanganate, which provides enhanced staining of cellular membranes [24], revealed a typical ultrastructure of overnight-grown (log-phase) yeast cells without desiccation (Fig. 1A). Stationary cells (3-day culture) without desiccation had more mitochondria than log-phase cells and the cristae of these mitochondria were more prominent (Fig. 1B). Additionally, hydrated stationary-phase cells possess one or a few dark-stained vacuoles and the vacuoles are often associated with the nucleus, forming the nucleus-vacuole junctions [25], which function as sites for piecemeal microautophagy that degrades portions of the yeast nucleus during starvation [26] (Fig. 1B). When cells were stored in the desiccated state for 14 days, significant structural changes were observed in both log- and stationary phase cells. In dried desiccation-sensitive log-phase cells, vacuoles diminished in almost all cells. ER membranes became curled, forming small circular structure (whorls), especially near the plasma membrane. These whorls were much smaller that observed in stationary desiccated cells (see next section). The gap between the inner and outer nuclear membrane expanded significantly (Fig. 1C). This phenomenon was also observed in dividing cells during log-phase growth. Circular ER membrane structures were mostly observed in the mother, but not the daughter cell (Fig. 1D). Desiccation also caused dramatic structural changes in desiccation-tolerant stationary cells, including increased ER membrane, reduced vacuole number and a reduction in mitochondrial cristae [16]. These changes are the major focus of this study and are described in details in the following sessions.

Desiccation triggers endoplasmic reticulum (ER) stress and unfolded protein response (UPR)

It has been reported that misfolded and unfolded proteins trigger endoplasmic reticulum (ER) stress, which in turn, induces unfolded protein response (UPR) in yeast [27]. When treated with the ER stressor dithiothreitol, the ER forms multi-layered, ring-shaped ER whorls [27-28]. ER whorls and whorl-like ER structure were also observed in stationary-phase yeast desiccated for 14 days (Figs. 2A, 2B), suggesting desiccation causes accumulation of unfolded/misfolded proteins [5], which induces ER stress, ultimately triggering UPR.

In budding yeast, UPR is mediated by the transmembrane endoribonuclease Ire1. Activation of Ire1 cleaves the mRNA of HAC1, which binds to a DNA sequence called UPR element to increase the expression of UPR target genes [29]. To further examine whether the ER whorls are related to UPR, we compared the cross sections of wild type, ire1Δ and hac1Δ mutants desiccated for 14 days. About 200 cell cross sections of each strain (from 3 independent experiments, 60-70 cells per experiment) were examined. ER whorls were observed in about 13% of wild type cells, but no ER whorls were observed in ire1Δ or hac1Δ mutant (Figs. 2C, 2D), further suggesting the ER whorls observed in desiccated cells (Fig. 2A, 2B) were induced by UPR during desiccation.

Desiccation causes expansion of endoplasmic reticulum

ER stress induces expansion of ER membrane and this expansion requires UPR signaling [30]. When 3-day stationary cells were desiccated for 14 days, a significant increase of ER membrane was observed in yeast cells with the ER membrane appearing to be disorganized and tangled around the nuclear membrane (Fig. 3A). This expanded and disorganized membrane was not observed in 3-day stationary cells prior to desiccation (Fig. 1B), nor in desiccated log-phase cells (Fig. 1C). These expanded membrane was different from the ER whorls, which were more organized and usually not associated with nuclear membrane. In desiccated stationary-phase cells, the ER membrane close to plasma membrane was often observed broken into small pieces, forming circular structures of 30-50 nm in diameter (Fig. 3B). One possibility is that the circular membrane structures are cross-sections of the ER whorls, or whorl-like structure observed in Figures 2A and 2B. These circular structure was rarely observed in ire1Δ or hac1Δ mutant.

Much less ER membrane accumulation, including both the disorganized membrane near nucleus and the circular membrane near plasma membrane, was observed in stationary-phase ire1Δ or hac1Δ mutant (Figs. 2C, 2D). We measured 6 cross sections from each strain (wild type, ire1Δ or hac1Δ) that contain the disorganized membrane near nucleus. It showed an approximately 40% less membrane accumulation in ire1Δ or hac1Δ mutant than in wild type. This reduced membrane expansion may be caused by blocking of UPR signaling in ire1Δ or hac1Δ mutant or be due to the fact that both ire1Δ and hac1Δ are involved in in phospholipid biosynthesis [31].

The nuclear and plasma membrane also underwent dramatic changes during desiccation in stationary-phase cells. Outward folding of the nuclear membrane was observed (Fig. 3C). This was not observed in desiccated log-phase cells and thus may be an adaptive response mounted by stationary-phase cells to cope with dehydration-induced cell shrinkage.

Another prominent feature of the 14-day desiccated stationary cells is the rupture of the nuclear envelop. The nuclear envelop opening was always associated with disorganized ER membrane (Figs. 3A, 3B). Similar to the wild type, broken nuclear membrane was also observed in desiccated stationary-phase hac1Δ and ire1Δ mutants (Figs. 2C, 2D). Examination of about 200 cells from TEM cross sections of each strain/growth condition (from 3 independent experiments, 60-70 cells per experiment) showed that 6-8% of cells displayed nuclear opening in desiccated wild type stationary cells or hac1Δ and ire1Δ cells, but not in desiccated log-phase cells (Figs. 1C, 1D).

Unfolded protein response does not affect the desiccation tolerance

We next checked the survival rates of yeast after 14 days of desiccation. The survival rate of the stationary wild type yeast was about 60%, but only 10% for log-phase cells. Interestingly, the survival rates of both ire1Δ and hac1Δ were slightly lower that the wild type, but the difference was not statistically significant (p > 0.05) (Fig. 4), suggesting that inactivation of UPR does not affect desiccation tolerance.

Vacuoles diminish during desiccation

The yeast vacuole is the largest organelle in a yeast cell and has similar functions to the lysosome in higher eukaryotes or the plant vacuole. In yeast the vacuole serves as the primary site for protein degradation and recycling, especially during starvation. Using TEM, we observed that hydrated 3-day stationary cells usually have one or a few dark-stained vacuoles (Fig. 1B). Examination of about 200 cells from TEM cross sections (from 3 independent experiments) showed that fourteen days after desiccation, over 90% of the stationary cells had no vacuoles (Figs. 2A-2B, 3). When observed, the vacuoles were lightly stained (Fig. 6A). Vacuoles were also absent in most of the log-phase desiccated cells (Figs. 1C, 1D).

Structural changes during the desiccation process

Next, we evaluated the ultrastructural changes during desiccation by examining stationary-phase yeast cells dried for 1, 2, 5 or 10 days. No significant difference was observed between cells dried for 1 or 2 days (Fig. 5A), in which nucleus was usually intact, similar to pre-desiccated cells, except that the mitochondrial cristae became less obvious. Vacuoles were mostly dark-stained, but light-stained spots were often observed inside the vacuoles (Fig. 5B), suggesting a possible initiation point for vacuole degradation. More light-stained vacuoles were observed in 5-day desiccated cells. Observation of about 200 cell cross sections from 3 independent experiments showed that engulfment of lipid droplet occurred in about 9-10% of either 2-day or 5-day-dried cells (Fig. 5C) and vacuoles were absent in more than 50% of 10-day desiccated cells and some lipid droplets were surrounded by ER membrane (Fig. 5D).

Endoplasmic reticulum (ER) and lipid droplets (LDs) dynamic in desiccated yeast cells

Lipid droplets (LDs) are ER–derived neutral lipid storage organelles [32]. They are universally conserved in both prokaryotes and eukaryotes and their biogenesis primarily occurs from ER, where newly synthesized neutral lipids emerge from and are surrounded by a phospholipid monolayer [33]. During desiccation, LDs were often observed to be associated with normal (Fig. 6A), disorganized (Fig. 6B), or circular ER membrane (Fig. 6C). It was observed that some of the circular structures had no membranes (arrow in Fig. 6C), suggesting that the ER membrane might be degraded and the free fatty acids were stored in LDs. If this is the case, we speculate that the overall LDs would increase during desiccation. To test this possibility, we checked the LD levels every 3 days for 15 days during desiccation. To our surprise, the LD level significantly decreased, rather than increased during the desiccation process (Fig. 6D, 7B). We reasoned that LDs might be consumed during desiccation to provide energy. This notion is supported by the TEM observation that LDs were engulfed during early stage of the desiccation by vacuoles (Fig. 5C), which degrade LDs by a process that resembles microautophagy [34-35].

Defects of lipid droplet synthesis reduce desiccation tolerance

To further test the possible role of LDs in desiccation tolerance, we examined yeast strains that are defective in LD formation. The yeast Pah1 is a homologue of mammalian lipin 1 protein, which plays important roles in glycerolipid biosynthesis. It regulates lipid droplet formation and nuclear/ER membrane growth [34]. Deletion of PAH1 causes significant decrease of number of LDs [36]. BODIPY staining revealed that the number of LDs in the 3-day stationary cells of pah1Δ was significantly less than that in wild type either prior to or after 14 days of desiccation (Fig. 7A, B). No significant change of LDs in pah1Δ mutant was observed after 14 days of desiccation. The survival rate of the pah1Δ mutant was significantly lower than the wild type (Fig. 7C). TEM observation revealed that after 14 days of desiccation, the LDs in pah1Δ cells lack clear boundaries and are usually smaller than those in wild type cells. (Fig. 7D). Measurement of 50 LD diameters from TEM cross sections showed that the average LD size/diameter of pah1Δ was about 1/3 of the wild type.

Pah1 is regulated by the Nem1-Spo7 phosphatase complex. Deletion of NEM1 or SPO7 partially inhibits the Pah1 activity and causes a phonotype similar to pah1Δ [37]. When the nem1Δ and spo7Δ deletion mutants in stationary phase were dried for 2 weeks, we found that their survival rates were much lower than in wild type cells, but higher than that of the pah1Δ (Fig. 7C). BODIPY staining showed that the number of LDs in nem1Δ or spo1Δ was also in between the wild type and pah1Δ (Fig. 7A). TEM observation of the 14-day dried nem1Δ or spo1Δ cells showed a structure similar to pah1Δ, except that LDs were larger than that in pah1Δ (Fig. 7D-E). In all three mutants, much less ER membrane accumulation was observed.

Next, we used the antifungal agent cerulenin, which inhibits the biosynthesis of fatty acids and steroids, to reduce LD formation [38]. In the presence of 4 µg/ml cerulenin, the number of LDs was significantly reduced (Figs. 7A, 7B). After 14 days drying, the survival rate of the cerulenin treated cells was significantly lower than the non-treated control (p < 0.01), similar to that of the pah1Δ mutant (Fig. 7B), further suggesting that accumulation of LDs is important to desiccation tolerance.

Using luciferase and prion propagation assays, Tapia and Koshland [5] demonstrated that desiccation induces protein misfolding, which is mitigated by the presence of trehalose. Misfolded proteins cause ER stress and trigger the UPR, which in turn, induces expansion of ER membrane [30]. In this study, we observed directly with TEM that desiccation causes the formation of ER whorls, a multi-layered, ring-shaped structure that has been reported under ER stress [27-28]. Our results show that the desiccation tolerance in ire1Δ or hac1Δ (deactivation of UPR) did not increase, but slightly decreased, suggesting that UPR may have an positive effect on desiccation tolerance by increasing the ER membrane, which may be used as a source for fatty acids as further discussed below.

One interesting phenomenon we observed in this study is the rupture of the nuclear envelop, which was often associated with the disorganized ER membrane (Fig. 3A). This phenomenon was also observed in ire1Δ or hac1Δ mutant, but not in desiccated log-phase cells, which had a much lower survival rate. It suggests that the opening of the nuclear envelop during desiccation might be a protection mechanism to mitigate the dehydration pressure that causes shrinkage of the nucleus. It cannot be ruled out that the cells with ruptured nuclear envelop are dead cells, but the fact that nuclear rupture was not observed in dried desiccation-sensitive log-phase cells suggests a putative connection to desiccation tolerance. One possibility for how this opening is generated is by the nucleus-vacuole junctions (Fig. 3B), which function as sites for piecemeal microautophagy of the nucleus [25]. During mitosis, the budding yeast nuclear membrane remains intact (termed closed mitosis), while in animals and plants, the nuclear membrane disassembles before chromosome segregation, which is termed open mitosis. The fission yeast Schizosaccharomyces japonicas, however, undergoes a semi-open mitosis, where the nuclear membrane partially open before chromosome segregation [39]. The opening of the nuclear membrane during desiccation resembles the semi-open mitosis in S. japonicas and a similar mechanism might be used to “repair” the ruptured nuclear envelope when condition allows. Another possibility for the observed nuclear membrane opening is that desiccation may cause compositional changes of nuclear membrane [17], making it difficult to be visualized by TEM using routine chemical fixation and staining [12].

The yeast vacuole plays an important role in nutrient storage, macromolecule degradation, detoxification, pH- and ion-homeostasis [40-41]. It is a dynamic organelle that responds to cell cycle and environment, especially the availability of nutrients. Log-phase cells usually have multiple, small vacuoles, while stationary cells, or cells deprived of carbon source, have fewer but relatively large vacuoles [42]. Interestingly, we observed that the majority of the vacuoles diminished after 14 days of desiccation in stationary-phase cells (Figs. 2A, 2B, 3A). The degradation of vacuoles appears to be initiated as early as 2 days after desiccation (Fig. 5B). The removal of vacuoles could be an adaptive response to the decreased cell volume due to the dehydration. During the desiccation process, there is no apparent external nutrients and water available. It is possible that the vacuoles are degraded to provide energy and water for preparative activities essential for survival during early stage of desiccation.

Lipid droplet (LD), an organelle surrounded by a monolayer of phospholipid, are found in all organisms. It is the primary cellular organelle for regulating the storage and hydrolysis of neutral lipids, including triglycerides and sterol esters. In yeast, the number of LDs increases in response to nutrient depletion. As a result, stationary cells have more LDs than log-phase dividing cells. A recent report showed that the number of LDs increased even when yeast cells were given a short period of glucose starvation by replacing glucose with a non-hydrolysable glucose analogue [43]. At stationary phase, fatty acids are released slowly from triglycerides and degraded via β-oxidation, to provide energy necessary for cellular maintenance [38]. In our current study, we observed that the level of LDs significantly decreased during desiccation (Fig. 6D), suggesting that the neutral fat was consumed by the desiccating cells. Catabolism of neutral fat, such as triglycerides, can provide the necessary energy for cells, while no other nutrients available. It is also possible these lipids could be used to repair or restore the membrane damages during rehydration [12].

Metabolic water, which results from the oxidation of cellular carbohydrates, proteins or lipids, is an important alternative water source for many organisms, especially animals in desert, insects and migrating birds [44-45]. Compared with carbohydrates and proteins, oxidation of fat produces the most amount of water (about 110 g of metabolic water per 100 g of fat) [46]. Unlike the desert animals that need the metabolic water to compensate the water insufficiency, the metabolic water, possibly from β-oxidation of the lipids, might be crucial to the survival of the desiccating yeast and keeping certain enzymes, such as trehalases, functional [5].

Another surprising finding of our study is that many lipid droplets in the desiccated cells were surrounded by circular ER membrane structures (Fig. 5C), and some of the circular membranes appeared to have no membrane. One possibility is that the phospholipid of the membrane might be converted into free fatty acids, which may be used as a source of energy and metabolic water. Another possibility is that the ER-LD contact is required to quickly break down lipids in the LDs, or to supply phospholipids for ER membrane expansion.

One limitation of our current study is the mixed population of desiccated cells, some were viable and some were dead. It is difficult to know whether the cellular changes observed by TEM are from viable or dead cells. While some of the phenotypes can be confirmed by comparing the desiccated log-phase (desiccation sensitive) and stationary-phase (desiccation tolerant) cells, or mutational analysis, it is ideal to improve the desiccation tolerance with near 100% viable desiccated cells. This will provide better assurance of the characteristics of viable cells.

In summary, our study demonstrated that yeast cells undergo significant structural changes in response to desiccation stress. The most prominent changes include the diminishing vacuoles, increased ER membrane, reduction in mitochondrial cristae, and dynamic changes in lipid droplet number and size. Our results suggest that the metabolism of the lipid droplets and membrane may play an important role in desiccation tolerance of the budding yeast.

Yeast strains and growth conditions

Yeast (Saccharomyces cerevisiae) cells were grown in YPD medium (1% yeast extract, 2% peptone and 2% dextrose) at 30°C with constant shaking at 250 rpm. The wild type parent strain BY4742 (Matα his3Δ leu2Δ met15Δ ura3Δ) and all deletion mutants were purchased from Thermo Scientific (Waltham, MA). For deletion mutants, each gene disruption was replaced with a KanMX module [47]. For cerulenin treatment, cerulenin (Thermo Scientific, Waltham, MA) stock solution (10 mg/ml in 100% ethanol) was diluted to a final concentration of 4 µg/ml into YPD medium. For controls, the same amount of 100% ethanol was added in place of cerulenin.

Sample preparation for transmission electron microscopy (TEM)

TEM samples were prepared as described previously [48] with the following modification. Yeast cells were cultured in YPD to log-phase (14-16 h, overnight culture) or to stationary phase (72 h, 3-day culture). All cultures were conducted at 30°C with constant shaking (250 rpm) using 15 ml round-bottom glass tubes (Fisher Scientific, Waltham, MA) filled with 5 ml of culture medium. Cells were processed for TEM directly, or desiccated (see below) before being processed for TEM. For desiccated cells, cells were resuspended in phosphate-buffered saline (PBS) (pH 7.2), vortexed for 30 secs and immediately centrifuged (×1,000 g for 5 min). Cells were then fixed in 2.5% (v/v) glutaraldehyde in PBS for 90 min at room temperature. Cells were washed twice with water and further fixed by 2% freshly prepared potassium permanganate in water for 90 min at room temperature. Fixed cells were stained with 2% uranyl acetate for 90 min, then dehydrated with 30%, 50%, 75%, 85%, 95% and 3 changes of 100% ethanol (60 min/step). Cells were transitioned with propylene oxide, infiltrated in Spur resin (Electron Microscopy Sciences, PA). The infiltration steps were 25% resin (in propylene oxide) for 4 hours, 50% resin overnight, 75% resin for 4 hours, then 100% resin (2 changes) overnight. The cells were treated twice (2 min/each) with a microwave (Pelco Bio-Wave 34700, Ted Pella, CA) while in 100% resin. The settings were 650 W with a restriction temperature of 43°C [49]. The prolonged fixation, dehydration infiltration are essential for the desiccated yeast cells. The resin was polymerized at 65°C overnight in the oven. Ultrathin sections of 60 nm were cut with a diamond knife, stained with lead citrate, and examined using a Hitachi H-7000 electron microscope (Hitachi High-Tech America, Schaumburg, IL) equipped with a cooled, high-resolution digital camera (Gatan Inc.). Majority of images were taken at ×8,000 magnification, which corresponds to a pixel size of 1.4 nm × 1.4 nm.

Desiccation tolerance assay

Cells were grown in 15 ml round-bottom glass tubes (Fisher Scientific, Waltham, MA) to log- or stationary phase in YPD medium. Cells were centrifuged (×1,000 g for 5 min) and the culture medium was discarded. The culture tubes without caps were placed in a humid chamber (23°C, 50% relative humility) and cells were allowed to desiccate for desired days. After desiccation, cells were resuspended in water, vortexed for 30 secs, then diluted to 3,000 cells/ml. 100 µl of cells were plated on YPD agar plates (1% yeast extract, 2% peptone, 2% dextrose and 2% agar) for 2-3 days, and the number of colonies were counted. Non-desiccated cells at the same growth conditions were used as controls. 3-4 plates were used for each strain or growth condition. Desiccation tolerance (survival rate)(%) was calculated as (number of colonies of desiccated cells)/(number of colonies of non-desiccated cells) X 100 from 3 independent experiments.

Estimation of membrane expansion

ImageJ software (https://imagej.nih.gov/ij/) was used to estimate membrane expansion from TEM cross sections. Expanded membrane near the nucleus of the 14-day desiccated stationary cells was manually selected and the area was measured. Six sections of each strain (wild type, ire1Δ and hac1Δ) from 2 independent experiments was used for the estimate.

Confocal microscopy imaging and quantification of lipid droplets

BODIPY 493 (Thermo Fisher Sci., Waltham, MA) was used to stain lipid droplets. For confocal microscopy imaging, BODIPY 493 was diluted with PBS to a final concentration of 1.25 µg/mL from its stock (1 mg/mL in 100% ethanol). Desiccated cells were briefly rehydrated and stained directly with the diluted BODIPY 493 for 5 min in the dark. Stained cells were imaged using a Zeiss 700 laser scanning confocal microscope (Zeiss, NY) with a 63X, 1.4 NA oil objective. Z-stacks of 1 µm step size were acquired and images were presented as maximum projections for fluorescence and the middle section for bright-field. For counting the number of LDs, at least 70 cells were counted from the maximum projected images of each experiment. Data were presented as mean ± standard derivation (SD) from 3 independent experiments.

For quantitative comparison of the LD fluorescence intensity, cells were first diluted and adjusted to OD₆₀₀ = 0.1. Cells were then stained with BODIPY 493 for 5 min in the dark. The relative fluorescence intensity was measured using a Tecan infinite M200 fluorescence microplate reader (Tecan US, Morrisville, NC) with excitation/emission at 495/530 nm. Data were presented as mean ± standard derivation (SD) from 3 independent experiments.

Statistical analysis

Data were presented as mean ± SD from 3 independent experiments. Student’s t-test was used to compare the difference between groups. P < 0.05 was considered as statistically significant.

Ethics declarations

Ethics approval and consent to participate

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Consent for publication

Not applicable.

Competing interests

The authors declare no conflicts of interests.

Acknowledgement:

This study was made possible by the Institutional Development Awards (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under Grant # P20GM121310 and 2P20GM103432.

Author contributions:

QR: Performed the experiments and analyzed the data. RB: Performed the experiments; TCB: Drafted and revised the article; ZZ: Conceived the experiments and revised the article.

Wharton DA. Anhydrobiosis. Current Biology. 2015;D25 (23): PR1114-R1116. DOI: https://doi.org/10.1016/j.cub.2015.09.047
Crowe, J.H., Hoekstra, F.A., and Crowe, L.M. Anhydrobiosis. Annu. Rev. Physiol. 1992;54, 579–599. doi: 10.1146/annurev.ph.54.030192.003051.
Boothby TC, Pielak GJ. Intrinsically Disordered Proteins and Desiccation Tolerance: Elucidating Functional and Mechanistic Underpinnings of Anhydrobiosis. Bioessays. 2017;39(11). doi: 10.1002/bies.201700119.
Boothby TC, Tapia H, Brozena AH, Piszkiewicz S, Smith AE, Giovannini I, Rebecchi L, Pielak GJ, Koshland D, Goldstein B. Tardigrades use intrinsically disordered proteins to survive desiccation. Molecular Cell. 2017;16;65(6):975-84.
Tapia H, Koshland DE. Trehalose is a versatile and long-lived chaperone for desiccation tolerance. Curr Biol. 2014;24(23):2758-66. doi: 10.1016/j.cub.2014.10.005.
Hoekstra FA, Golovina EA, Buitink J. Mechanisms of plant desiccation tolerance. Trends Plant Sci. 2001;6(9):431-8.
Panek AD. Trehalose metabolism and its role in Saccharomyces cerevisiae, J. Biotechnol. 1985;3:121-130.
Panek AD. Trehalose metabolism - new horizons in technological applications. Brazil. J. Med. Biol. Res. 1995;28:169-181.
Eleutherio E, Panek A, de Mesquita JF, Trevisol D, Magalhães R. Revisiting yeast trehalose metabolism, Curr. Genet. 2015;61:263-274.
Beker MJ, Rapoport AI. Conservation of yeasts by dehydration. Adv. Biochem. Engin./Biotechnol. 1987;35:127-171.
Rapoport A. Anhydrobiosis and dehydration of yeasts. In: Biotechnology of Yeasts and Filamentous Fungi (Ed. A. Sibirny). Springer International Publishing, 2017, 87-116.
Rapoport A, Golovina EA, Gervais P, Dupont S, Beney L. Anhydrobiosis: Inside yeast cells. Biotechnology Advances 2019;37:51-67.
Dupont S, Rapoport A, Gervais P, Beney L. The survival kit of Saccharomyces cerevisiae for anhydrobiosis (Review). Applied Microbiology and Biotechnology, 2014;98: 8821-8834.
de Jesus Pereira E, Panek AD, Eleutherio ECA. Protection against oxidation during dehydration of yeast. Cell Stress Chaperones. 2003;8(2):120-124.
Ingram, J. and Bartels, D. The molecular basis of dehydration tolerance in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 1996;47, 377–403
Calahan D, Dunham M, DeSevo C, Koshland DE. Genetic analysis of desiccation tolerance in Saccharomyces cerevisiae. Genetics. 2011;189(2):507-19. doi: 10.1534/genetics.111.130369.
Zikmanis PB, Auziņa LP, Auzáne SI and Beker MJ. Relationship between the fatty acid composition of lipids and the viability of dried yeast Saccharomyces cerevisiae. Eur. J. Appl. Microbiol. Biotechnol. 1982;15: 100–103.
de Nobel H, Ruiz C, Martin H, Morris W, Brul S, Molina M, Klis FM. Cell wall perturbation in yeast results in dual phosphorylation of the Slt2/Mpk1 MAP kinase and in an Slt2-mediated increase in FKS2-lacZ expression, glucanase resistance and thermotolerance. Microbiology. 2000;146 (Pt 9):2121-2132. doi: 10.1099/00221287-146-9-2121.
Gray JV, Petsko GA, Johnston GC, Ringe D, Singer RA &Werner-Washburne M. ‘Sleeping beauty’: quiescence in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 2004;68: 187–206.
Koster KL, Webb MS, Bryant G, Lynch DV. Interactions between soluble sugars and POPC (1-palmitoyl-2-oleoylphosphatidylcholine) during dehydration: vitrification of sugars alters the phase behavior of the phospholipid. Biochim. Biophys. Acta 1994;1193:143-150. DOI: 10.1016/0005-2736(94)90343-3
Koster KL, Maddocks JK, Bryant G. Exclusion of maltodextrins from phosphatidylcholine multilayers during dehydration: effects on membrane phase behaviour. Eur. Biophys. J. 2003;32:96-105. DOI: 10.1007/s00249-003-0277-z
Wolfe J, Bryant G. Freezing, drying, and/or vitrification of membrane-solute-water systems. Cryobiol. 1999;39:103-129. Doi.org/10.1006/cryo.1999.2195
Erkut C, Penkov S, Khesbak H, Vorkel D, Verbavatz JM, Fahmy K, Kurzchalia TV. Trehalose renders the dauer larva of Caenorhabditis elegans resistant to extreme desiccation. Curr Biol. 2011;21(15):1331-6. doi: 10.1016/j.cub.2011.06.064.
Wright R. Transmission electron microscopy of yeast. Microsc Res Tech. 2000;51(6):496-510. doi: 10.1002/1097-0029(20001215)51:6<496::AID-JEMT2>3.0.CO;2-9
Kvam E and Goldfarb DS. Structure and function of nucleus-vacuole junctions: outer-nuclear-membrane targeting of Nvj1p and a role in tryptophan uptake. J Cell Sci. 2006;119(Pt 17):3622-33.
Roberts, P., Moshitch-Moshkovitz, S., Kvam, E., O'Toole, E., Winey, M. and Goldfarb, DS. Piecemeal microautophagy of the nucleus in Saccharomyces cerevisiae. Mol. Biol. Cell 2003;14, 129-141
Bernales S, McDonald KL, Walter P. Autophagy counterbalances endoplasmic expansion during the unfolded protein response. PLoS Biol. 2006 Nov;4(12):e423. doi: 10.1371/journal.pbio.0040423
Schuck S, Gallagher CM, Walter P. ER-phagy mediates selective degradation of endoplasmic reticulum independently of the core autophagy machinery. J Cell Sci. 2014;127(Pt 18):4078-88. doi: 10.1242/jcs.154716.
Welihinda AA and Kaufman RJ. The unfolded protein response pathway in Saccharomyces cerevisiae. Oligomerization and trans-phosphorylation of Ire1p (Ern1p) are required for kinase activation. J Biol Chem. 1996;271(30):18181-7Tran
Schuck S, Prinz WA, Thorn KS, Voss C, Walter P. Membrane expansion alleviates endoplasmic reticulum stress independently of the unfolded protein response. J Cell Biol. 2009;187(4):525-36. doi: 10.1083/jcb.200907074.
Kaufman RJ. Stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controls. Genes Dev. 1999;13(10):1211-33
Olzmann, J.A., and P. Carvalho. Dynamics and functions of lipid droplets. Nat. Rev. Mol. Cell Biol. 2019;20, 137–155; doi:10.1038/s41580-018-0085-z
Choudhary V, Ojha N, Golden A and Prinz WA. A conserved family of proteins facilitates nascent lipid droplet budding from the ER. J Cell Biol. 2015;211, 261–271.
Teter SA, Eggerton KP, Scott SV, Kim J, Fischer AM, Klionsky DJ. Degradation of lipid vesicles in the yeast vacuole requires function of Cvt17, a putative lipase. J Biol Chem. 2001;276(3):2083-7. doi: 10.1074/jbc.C000739200.
van Zutphen T, Todde V, de Boer R, Kreim M, Hofbauer HF, Wolinski H, Veenhuis M, van der Klei IJ, Kohlwein SD. Lipid droplet autophagy in the yeast Saccharomyces cerevisiae. Mol Biol Cell. 2014;25(2):290-301. doi: 10.1091/mbc.E13-08-0448.
Adeyo O, Horn PJ, Lee S, Binns DD, Chandrahas A, Chapman KD, Goodman JM. The yeast lipin orthologue Pah1p is important for biogenesis of lipid droplets. J Cell Biol. 2011;192(6):1043-55. doi: 10.1083/jcb.201010111.
Santos-Rosa H, Leung J, Grimsey N, Peak-Chew S, Siniossoglou S. The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth. EMBO J. 2005;24(11):1931-41. DOI: 10.1038/sj.emboj.7600672
Kurat CF, Natter K, Petschnigg J, Wolinski H, Scheuringer K, Scholz H, Zimmermann R, Leber R, Zechner R, Kohlwein SD. Obese yeast: triglyceride lipolysis is functionally conserved from mammals to yeast. J Biol Chem. 2006;281(1):491-500. DOI: 10.1074/jbc.M508414200
Boettcher B, Barral Y. The cell biology of open and closed mitosis. Nucleus. 2013;4(3):160-5. doi: 10.4161/nucl.24676.
Broach JR, Pringle JR, Jones EW. The molecular and cellular biology of the yeast Saccharomyces. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press, 1991
Jones EW, Webb GC, Hiller MA. Biogenesis and function of the yeast vacuole. Molecular biology of the yeast Saccharomyces cerevisiae Vol. III. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, 1997;p 363– 469
Hecht KA, O'Donnell AF, Brodsky JL. The proteolytic landscape of the yeast vacuole. Cell Logist. 2014;4(1):e28023.
Marinia G, Nüskea E, Lenga W, Albertia S, Pigino G. Reorganization of budding yeast cytoplasm upon energy depletion. Mol Biol Cell. 2020;31(12):1232-1245. doi: 10.1091/mbc.E20-02-0125.
Edney EB. Metabolic Water. In: Water Balance in Land Arthropods. Zoophysiology and Ecology, 1977;vol 9. Springer, Berlin, Heidelberg
Li H, Yu C, Wang F, Chang SJ, Yao J, Blake RE. Probing the metabolic water contribution to intracellular water using oxygen isotope ratios of PO4. Proc Natl Acad Sci U S A. 2016;113(21):5862-7. doi: 10.1073/pnas.1521038113.
Mellanby K. Metabolic water and desiccation. Nature 1942;150, 21. https://doi.org/10.1038/150021a0.
Brachmann CB, Davies A, Cost JG, Caputo E, Li J, Hieter P, Boeke JD. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast. 1998 14(2):115-32.
Yang H, Ren Q, Zhang Z. Chromosome or chromatin condensation leads to meiosis or apoptosis in stationary yeast (Saccharomyces cerevisiae) cells. FEMS Yeast Res. 2006;6(8):1254-63.
Giberson RT. Vacuum-assisted microwave processing of animal tissue for electron microscopy. Microwave Technique and Protocols (Giberson RT & Demaree RS, eds), 2001;pp. 13–23. Humana Press, Totowa, NJ.

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Membrane and lipid metabolism plays an important role in desiccation resistance in the yeast Saccharomyces cerevisiae

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