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Research Article
Adriano Teixeira de Oliveira
IFAM: Instituto Federal de Educacao Ciencia e Tecnologia do Amazonas
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4988-9878
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This study aimed to determine the dietary lysine requirements of juvenile Colossoma macropomum tambaqui based on growth performance. We also evaluated gut and hepatic histomorphometry as well as blood metabolites in accordance with the increased levels of dietary lysine. The juveniles (33.88 ± 2.47 g) were fed until apparent satiation with diets containing 6.60, 9.72, 12.84, 15.96, 19.08 and 22.20 g/kg of lysine. Fish were randomly distributed in groups of 10 fish per tank and assays were performed in triplicate, during 90 days. Tambaqui fed with 15.96 g/kg dietary lysine showed higher final weight (p = 0.001) and optimized feed conversion ratio (p = 0.001). Morphohistological modifications were present in livers of fish fed with low levels of lysine. In the proximal intestine, mucosa layer density was greater at the level of 15.96 g/kg (p = 0.001). In the middle intestine, height (p = 0.001) and perimeter (p = 0.001) of the villi were greater at low levels of lysine (respectively, 9.72 and 12.84 g/kg dietary lysine). Tambaqui fed with 15.96 g/kg of lysine achieved higher plasma protein concentrations (p = 0.01). Using the second-order polynomial regression analysis as support, and based on protein efficiency rate and body weight gain, dietary lysine requirement for juvenile tambaqui was calculated as 15.4–15.6 g/kg of diet (5.7–5.8% of dietary protein).
Keywords
fish farming, nutrition, crystalline amino acid, animal production.
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CURRENT STATUS: Under Review
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Posted 10 May, 2021
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Editorial decision: Major revisions
On 24 May, 2021
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Received 06 May, 2021
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This preprint is under consideration at Veterinary Research Communications. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Adriano Teixeira de Oliveira
IFAM: Instituto Federal de Educacao Ciencia e Tecnologia do Amazonas
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4988-9878
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 10 May, 2021
No community comments so far
Editorial decision: Major revisions
On 24 May, 2021
Reviews received
Received 06 May, 2021
Reviewers invited
Invitations sent on 06 May, 2021
Editor assigned
On 03 May, 2021
First submitted
On 02 May, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This study aimed to determine the dietary lysine requirements of juvenile Colossoma macropomum tambaqui based on growth performance. We also evaluated gut and hepatic histomorphometry as well as blood metabolites in accordance with the increased levels of dietary lysine. The juveniles (33.88 ± 2.47 g) were fed until apparent satiation with diets containing 6.60, 9.72, 12.84, 15.96, 19.08 and 22.20 g/kg of lysine. Fish were randomly distributed in groups of 10 fish per tank and assays were performed in triplicate, during 90 days. Tambaqui fed with 15.96 g/kg dietary lysine showed higher final weight (p = 0.001) and optimized feed conversion ratio (p = 0.001). Morphohistological modifications were present in livers of fish fed with low levels of lysine. In the proximal intestine, mucosa layer density was greater at the level of 15.96 g/kg (p = 0.001). In the middle intestine, height (p = 0.001) and perimeter (p = 0.001) of the villi were greater at low levels of lysine (respectively, 9.72 and 12.84 g/kg dietary lysine). Tambaqui fed with 15.96 g/kg of lysine achieved higher plasma protein concentrations (p = 0.01). Using the second-order polynomial regression analysis as support, and based on protein efficiency rate and body weight gain, dietary lysine requirement for juvenile tambaqui was calculated as 15.4–15.6 g/kg of diet (5.7–5.8% of dietary protein).
Figure 1
Figure 2
Figure 3
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