See the published version of this preprint at Mobile DNA.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Research
Heinrich zu Dohna
American University of Beirut
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0474-9628
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
LINE-1 (Long Interspersed Nuclear Elements, L1) retrotransposons are the only autonomously active transposable elements in the human genome. The evolution of L1 retrotransposition rates and its implications for L1 dynamics are poorly understood. Retrotransposition rates are commonly measured in cell culture-based assays, but it is unclear how well these measurements provide insight into L1 population dynamics. This study applied comparative methods to estimate parameters for the evolution of retrotransposition rates, and infer L1 dynamics from these estimates.
Results
Our results show that the rates at which new L1s emerge in the human population correlate positively to cell-culture based retrotransposition activities, that there is an evolutionary trend towards lower retrotransposition activity, and that this evolutionary trend is not sufficient to counter-balance the increase in L1s resulting from continuing retrotransposition.
Conclusions
Together, these findings support a model of the population-level L1 retrotransposition dynamics that is consistent with prior expectations and indicate the remaining gaps in the understanding of L1 dynamics in human genomes.
Keywords
Long Interspersed Nuclear Elements, retrotransposition
Figure 1
Figure 2
Figure 3
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Mobile DNA.
Version 1
Posted 25 May, 2021
No community comments so far
Review #3 received
Received 18 Jun, 2021
Editorial decision: Major revision
On 18 Jun, 2021
Review #2 received
Received 08 Jun, 2021
Reviewer #3 agreed
On 06 Jun, 2021
Review #1 received
Received 05 Jun, 2021
Reviewer #2 agreed
On 28 May, 2021
Reviews received
Received 27 May, 2021
Reviewer #1 agreed
On 26 May, 2021
Reviewers invited
Invitations sent on 24 May, 2021
Editor assigned
On 24 May, 2021
Editor invited
On 23 May, 2021
Submission checks complete
On 23 May, 2021
First submitted
On 12 May, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Molecular Biology
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Mobile DNA.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Heinrich zu Dohna
American University of Beirut
Corresponding Author
ORCiD: https://orcid.org/0000-0003-0474-9628
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Mobile DNA.
Version 1
Posted 25 May, 2021
No community comments so far
Review #3 received
Received 18 Jun, 2021
Editorial decision: Major revision
On 18 Jun, 2021
Review #2 received
Received 08 Jun, 2021
Reviewer #3 agreed
On 06 Jun, 2021
Review #1 received
Received 05 Jun, 2021
Reviewer #2 agreed
On 28 May, 2021
Reviews received
Received 27 May, 2021
Reviewer #1 agreed
On 26 May, 2021
Reviewers invited
Invitations sent on 24 May, 2021
Editor assigned
On 24 May, 2021
Editor invited
On 23 May, 2021
Submission checks complete
On 23 May, 2021
First submitted
On 12 May, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Molecular Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Mobile DNA.
Background
LINE-1 (Long Interspersed Nuclear Elements, L1) retrotransposons are the only autonomously active transposable elements in the human genome. The evolution of L1 retrotransposition rates and its implications for L1 dynamics are poorly understood. Retrotransposition rates are commonly measured in cell culture-based assays, but it is unclear how well these measurements provide insight into L1 population dynamics. This study applied comparative methods to estimate parameters for the evolution of retrotransposition rates, and infer L1 dynamics from these estimates.
Results
Our results show that the rates at which new L1s emerge in the human population correlate positively to cell-culture based retrotransposition activities, that there is an evolutionary trend towards lower retrotransposition activity, and that this evolutionary trend is not sufficient to counter-balance the increase in L1s resulting from continuing retrotransposition.
Conclusions
Together, these findings support a model of the population-level L1 retrotransposition dynamics that is consistent with prior expectations and indicate the remaining gaps in the understanding of L1 dynamics in human genomes.
Figure 1
Figure 2
Figure 3
Loading...