Idiosyncratic response of yeasts, recovered from oropharyngeal lesions of HIV patients, to prophylactic antifungal agents, in South Western Uganda

Background: Although cutting edge technologies have advanced overall knowledge of microbial infections, what microorganisms will do when challenged with surface active agents is incompletely understood and understanding the characteristic uniqueness of an organism when challenged with antimicrobial agents derails antibiotic stewardship programs in resource limited settings. T his study was therefore designed to determine the idiosyncratic in-vitro response of yeast strains, challenged with selected routine antifungal agents used for routine prophylactic management of HIV infected Methods: A total of 304 (235 females and 69 males) patients with different conditions were recruited. The analysis performed included: cultivation on Sabouraud Dextrose Agar, Corn Meal Agar, Potato Dextrose Agar, germ-tube test, chlamydospore production, test for ability to grow at 45 o C. For further morphologic identification, yeasts were grown on CHROMagar Candida medium at 37 + 2 0 C for 24-48 hours and sub-cultured on Pal’s agar. The minimum inhibitory concentration (MIC) of the isolates were determined by the E-test stable agar gradient method (AB BIODISK, Solna, Sweden) Results: The 304 (235 females and 69 males) recruited patients with different oral lesions consented to this study and 304 sample surveyed was guided by the upper limit required to give 95% level of confidence at an expected prevalence of about 74% (Agwu et al, 2008) using : Sample size (N) = Z 2 P (100-P)/D2 giving 296 samples, (Epi-info version 3.2 data-base; 1995), where Z is a constant given as (1.96), P is expected prevalence (74%), and D is acceptable error (5%). Informed consent was sought and obtained from the following: Uganda National Council of Science and Technology, Kampala International University Research and Ethics Committee, HIV patients of The AIDS Support Organizations,. Conclusion : Candida albicans remain significantly susceptible to tested antifungal agents. Emerging dose dependent susceptibility in non albicans oropharyngeal yeast strains is interesting in

albicans remain signi cantly susceptible to tested antifungal agents.Emerging dose dependent susceptibility in non albicans oropharyngeal yeast strains is interesting in this era of increasing treatment failures due to drug resistance.Pattern of resistance recorded may suggest signi cant contribution of yeast in the recurrent or delayed oropharyngeal lesions of HIV population studied.Sustained surveillance with MIC which impacts treatment outcome is recommended Background Oropharyngeal Candidiasis (OC) is one of the Acquired immunode ciency syndrome (AIDS) de ning opportunistic infections caused by Human Immunode ciency viruses (HIV).Recurrent oropharyngeal Candidiasis common among HIV sero-positive patients may be associated to: drug-resistance-related treatment failure (1); non-compliance to drug prescription guidelines (2); use of sub-lethal drug concentration (3)(4); re-infection of microbial disease aetiology in disease Endemic regions (5) or unru ed progression of HIV disease to AIDS (1-2).To succeed in oropharyngeal lesion management in the immune-suppressed HIV disease, intervention must be based on what is known about the population structure of the oral microecology and yeast resistance to treatment regimen (6)(7)(8)(9).This should then give rise to bases for new drug development plans, in answer to identi ed challenges and unmet therapeutic needs so that sustainable intervention for today and tomorrow outbreaks can be contained (10) To reduce rates of treatment failure, systematic treatment should take place during the fungal growth phase when constant breakdown and re-synthesis of cell walls constituent giving polyenes more access to the sensitive targets of host cells (11).Polyenes and Azoles remain the antifungal agents most commonly used to treat Candida infections in the immunocompromised patients living in Uganda.The polyenes like amphotericin B and nystatin are fungicidal agents that bind preferentially to the ergosterol found in fungal membranes (12)(13).This disrupts the integrity of the cellular membrane, causing cell death.Resistance to polyenes may develop by modifying the ergosterol molecule or its membrane content and mechanisms of yeasts cells resistance to polyenes include replacement of the polyenebinding ergosterol, cholesterol, or stigmasterol with polyene-less binding 3-hydroxy or 3-oxo sterols (1,(3)(4).. Fluconazole and ketoconazole are fungistatic agents that inhibit cytochrome P450 14a-demethylase, a component of the sterol biosynthesis pathway (11,(14)(15)(16).New azole and triazole derivatives [voriconazole, ER-30346, and D0870] and SCH-56592, which is a hydroxylated analogue of Itraconazole may be available but they are not reachable to the population that need them in resource limited counties like Uganda.
Accepting to live with HIV (17) may be understandable because HIV infection have no clear routine cure yet, but no one would accept to live with recurrent oropharyngeal lesions associated with yeast strains when presumed cure are available (18).Management of HIV infected patients with recurrent oropharyngeal lesions remain a challenge because of the inherent dilemma associated with persistence of infection.Thus, It is not easy to determine the cause attribution factors to either HIV disease progression (19) or treatment failure associated with resistance of microbial oral pathogens found in the microecology of the oral cavity (1,(8)(9)20).There is no anti-fungal medication widely available for the majority of HIV-infected patients with oral lesions in Uganda.Majority of HIV patients in Uganda are on prophylactic drugs depending on the disease staging status.They are either routinely given antibacterial, antifungal or antiviral either separately of together.
There is no organized surveillance to update healthcare managers with performance of antimicrobial agents used in prophylactic management of HIV associated diseases.While in vivo assessment of drug performance when administered to sick people to treat associated infections remain relevant, such experiment depends on enough representative in-vitro data.This study was therefore designed to determine the idiosyncratic in-vitro response of yeast strains, challenged with selected routine antifungal agents used for routine prophylactic management of HIV infected TASO patients in Uganda, with the ultimate goal of ascertaining the performance of the prophylactic medications and impacting the outcome of future intervention.

Methods
Three TASO centres in Masaka, Rukunguri, and Mbarara districts of were used for this investigation.
Patient's sampled were TASO HIV patients with clinically diagnosed oral lesions and receiving cotrimoxazole, Nystatine and clotrimazole prophylaxis.Approvals were obtained from Uganda National Council of Sciences and technology, The AIDS Support Organizations (TASO), TASO clients (patients) through their informed consent.A total of 304 (235 females and 69 males) patients with different oral lesions consented to participate in this study and were therefore recruited.This 304 sample size surveyed was guided by the upper limit required to give 95% level of con dence at an expected prevalence of about 74% (6) using the precise prevalence formula: Sample size (N) = Z 2 P (100-P)/D2 giving 296 samples, (Epi-info version 3.2 data-base; 1995), where Z is a constant given as (1.96), P is expected prevalence (74%), and D is acceptable error (5%).Eight (8) more samples was added arbitrarily just in case of drop out and unseen error making a total of 304 samples.The mouth examination of the TASO clients was done by previously trained and calibrated oral clinicians according to WHO standards (21).
All analyses for yeast identi cation were done at the Microbiology Department, Kampala International University Western Campus.The analysis performed included: cultivation on Sabouraud Dextrose Agar, Corn Meal Agar, Potato Dextrose Agar, germ-tube test, chlamydospore production, test for ability to grow at 45 o C. For further morphologic identi cation of yeasts, they were rst grown on CHROMagar Candida medium at 37+ 2 0 C for 24-48 hours and sub-cultured on Pal's agar.(22).Suspect colonies of yeast strains were further identi ed and differentiated by biochemical test using sugar assimilation test pattern (ID32 C) for Candida species provided by BioMereux (R) Paris, France.

Determination of MIC of fungal Isolates by E-test
The minimum inhibitory concentration (MIC) of the isolates were determined by the E-test stable agar gradient method (AB BIODISK, Solna, Sweden) which has been standardized and comparable with Clinical Laboratory Standard Institute (CLSI) and EUropean Committee for Antimicrobial Susceptibility Testing (EUCAST) (23)(24).This involved placing a plastic strip containing a gradient of an antifungal agent on the surface of an inoculated RPMI (R) agar plate.The drug diffuses into the RPMI (R) agar and establishes a stable concentration gradient.Inhibition of fungal growth produces an ellipse, and the MIC is read where the ellipse intersects the test strip.
The Randomized Block Design (RBD) and Completely Randomized Design (CRD) (α = 0.1 or 99% level of con dence) summarized as [Total sum of squares (SS T ) = Sum of squares due to different effects (SS D ) + Error sum of effects (SS E )] ( 25), for one observation per treatment were used to test for the relationship between each yeast strains isolated and the district of isolation and between each yeast strains isolated and antifungal agents in different districts studied.The relation between each yeast response to antifungal used were compared to responses from other yeasts within same district.Fisher's least signi cant difference (FLSD; α = 0.05) and Duncan's Multiple Range test (DMRT; α = 0.01) were used to test for levels of signi cance of any relationship identi ed after computation with CRD and RBD.
MIC Result interpretation, based on CLSI formerly (NCCLS) guideline for antifungals were as follows: Fluconazole: MIC<8µg/ml =sensitive; Fluconazole: MIC>8µg/ml and ≤32µg/ml = sensitive dose dependent (SDD) and MIC>32µg/ml = resistant.For Itraconazole: MIC ≤ 0.125µg/ml = sensitive; MIC> 0.125µg/ml and ≤ 0.5 µg/ml = sensitive dose dependent and MIC > 0.5 µg/ml = Resistant.For Amphotericin B: Candida species isolates with Amphotericin B with MIC > 0.5µg/ml should be considered resistant because speci c breakpoints have not been proposed and intra-laboratory variations mandates the use of reference isolates with known resistance.
Figures 1-6 shows representative pictures of yeast response pattern to tested antifungals and so formed the basis for which different yeast strains were declared either sensitive, sensitive dose dependent or resistant to uconazole, Itraconazole and amphotericin B respectively.The average MIC results across the Districts surveyed, show that all Candida albicans strains were sensitive to the azoles except in Masaka District where C albicans showed sensitive-dose dependent MIC of (0.14 µg/ml) to Itraconazole.In selection, Amphotericin B in Masaka showed sensitive MIC of 0.125 µg/ml while C. glabrata was: resistant to Itraconazole (MIC>32.0 µg/ml); and its sensitivity to Fluconazole (MIC = 12.0 µg/ml) was dose dependent in all districts surveyed.(Table 1) Candida sake showed a uniform dose dependent sensitivity to Itraconazole (MIC = 0.5 µg/ml), uconazole (MIC = 0.9 µg/ml) and Amphotericin B (0.25 µg/ml).C. parapsilosis showed uniform sensitivities to Itraconazole and Fluconazole (MICs = 0.01 µg/ml and 0.38 µg/ml).C. norevegensis showed uniform resistance to both Amphotericin B (MIC=0.75 µg/ml) and Itraconazole (mean MIC = 2.0 µg/ml).However, C. norvegensis sensitivity to uconazole (MIC=8.0)was dose dependent.C. tropicalis and Saccharomyces kluyverii was sensitive to all the antifungals used in all 4 Districts with mean MICs ranging from 0.05 µg/ml to 1.0 µg/ml.Saccharomyces cerevesiae was sensitive to Fluconazole (MIC = 2.0 µg/ml) and resistant to Amphotericin B (mean MIC = 0.9 µg/ml) in all the Districts.Finally, Zygosaccharomyces species was uniformly resistant to Itraconazole (MIC = >32 µg/ml) and sensitive to Fluconazole (MIC = 0.75 µg/ml) in all Districts surveyed (Table 1) When MIC results within each District and among the 9 representative yeast strains tested were compared, C. parapsilosis in Mbarara District, showed more sensitivity to Itraconazole, (MIC = 0.01 µg/ml).This sensitivity was followed by: C. albicans and C. tropicalis (MIC = 0.05 µg/ml each).C. sake (MIC = 0.5 µg/ml) showed dose dependent sensitivity.C. glabrata, C. norvegensis and Zygosaccharomyces sp were all resistant to Itraconazole with MIC values of >32.0 µg/ml, 2.0 µg/ml and >32.0 µg/ml respectively.
District of isolation of yeast strains signi cantly (p<0.05)impacted on MIC results as different MIC values were obtained after testing similar yeast strains from different districts against same anti-fungal agents.
Thus, intra-species variations in average MIC values were noted among the yeast strains from different Districts.Itraconazoles tested against C. albicans showed varying MICs values of: 0.05 µg/ml in Mbarara District; 0.03 µg/ml in Rukungiri District and 0.14 µg/ml in Masaka District.Fluconazole tested against C. albicans also showed varying MICs of: 0.5 in Mbarara District; 0.99 µg/ml in Rukungiri District and 0.14 µg/ml in Masaka District.Itraconazole MIC tested against C. norvegensis was 2.0 µg/ml in Mbarara and Rukungiri Districts but was 0.125 µg/ml in Masaka District.Fluconazole tested against C. tropicalis showed different MICs of: 1.0µg/ml in Mbarara District and 0.38 µg/ml in Rukungiri and Masaka Districts.

Discussion
Although cutting edge modern technologies have advanced overall knowledge of microbial infections with consequent better outcome, what microorganisms will do when challenged with different surface active agents is still vague and incompletely understood.Disease diagnostic precision remain far below the minimum threshold needed to yield meaningful and reliable outcome especially in resource poor settings.Understanding the idiosyncrasy or characteristic uniqueness an organism when challenged with antimicrobial agents is a hug challenge to many antibiotic stewardship programs in resource limited settings.Perhaps the unpredictability of microbial response to surface active agent's emphasis the need to embrace the use of tests that are more sensitive and speci c for more result oriented outcome.
Oropharyngeal lesions remain signi cant health challenge (26) that HIV infected population must overcome for a better life, because there are many questions that needs hard-to-get answers about possible disease comorbidities that could delay or prolong the healing of such oropharyngeal lesions.Con rmatory investigations are needed to determine if prolong or delayed healing of oropharyngeal lesions could be linked to: drug-resistance (1), non-adherence to prescription guidelines (2)), abuse of antibiotics with sub-lethal doses (3-4), re-infection (5), progression of HIV disease to AIDS (1-2) or resistant opportunistic infections (27).With the increasing notorious nature of yeast as predominant opportunistic pathogens in oropharyngeal lesions of HIV/AIDS patients, it becomes necessary to determine the characteristic vulnerability of yeasts to routine prophylactic antifungal agents to achieve better intervention.
In this study, Candida albicans were sensitive to uconazole, Itraconazole and Amphotericin B respectively (Table 1; Fig 1, 4-5).Dose dependent sensitivity to various antifungal agents used were also shown by Candida glabrata (MIC=12.0),Candida sake (MIC=0.5)and Candida norevegensis (MIC=8.0)respectively (Fig 2).This may indicate possible emergence of antifungal drug resistance.Again, it is not clear why yeast strains development of dose dependent sensitivity were different in different TASO centres because disease management policies are presumed to be the same in these centres.The observation that same yeast species from different centres under same prescription and TASO management policy yielded different MIC, shows unique idiosyncratic nature of yeasts response to treatment regimen and this makes it hard to extrapolate results across centres.Thus inter regional surveillance to monitor the emergence and re-emergence of yeast susceptibility in a locale antimicrobial stewardship program is urgently needed.
While C. albicans remain the leading aetiology of candidiasis (28-30), reports that infections caused by other non albicans yeast species are globally gaining more research attention with species varying between different geographic regions.This is problematic, because resistance to uconazole may also mean resistance to other azoles and resistance are commonly controlled by point mutation and e ux pumps (31)(32).The rates of azole resistance may differ between institutions due to prescribing patterns of clinicians for both the treatment of and prophylaxis against pathogenic candidiasis (33).In India, C. tropicalis is the predominant species, and rates of uconazole resistance may vary signi cantly (34).The prevalence of C. parapsilosis was similar to C. albicans in some Chinese hospitals (35).Fluconazole susceptibility is also highly variable between institutions, with some reporting as high as 50% in intensive care units (35).
In Table 1 and Fig 1-5, all yeast isolates showed dose dependent sensitivity (DDS), susceptibility to both azoles and polyene antifungals, while C. glabrata, C. norvegensis and Zygosaccharomyces species exhibited reduced susceptibly (SDD) to uconazole and were resistant to Itraconazoles in all studied districts.While the advantages of prophylaxis in HIV infected population cannot be denied, emergence of sub-population showing reduced sensitivity may be announcing the imperatives of a new prophylactic algorithms in immunocompromised HIV infected population living in resource limited countries like Uganda.Again, while prophylactic medication usually have clearly visible entry plan for infected patients, exit plan are often not stated, omitted or ignored by drug prescribers in Uganda.Patients on prophylactic drugs therefore continue to use the drugs even when the e cacy of the drugs decreases (36).
Non-albicans species, including C. glabrata have known innate resistance to antifungal drugs (4,(37)(38).The discrepancy between this report (Table 1) of reduced susceptibility and known innate resistance of C. glabrata may be explained by a possibility of misdiagnoses with other phenotypically similar yeast strains.Thus, the phenotypic similarity between C. glabrata, C. nivariensis and C. bracarensis, could be misdiagnosed as C. glabrata in the absence of good con rmatory typing methods (39).There may be emerging generation of Candida glabrata which may not be highly resistant to conventional antifungals as previously anticipated because sensitivity to Candida glabrata reports are beginning to emerge from different geographical settings (37)(38)(40)(41)(42). Reports of C. glabrata resistant from some African countries include Cameroon (43), Ethiopia (44) and Tanzania (45), while reduced or dose dependent sensitivity to C. glabrata has been reported in South Africa (43) and Nigeria (42).The result of these study is similar to most reported studies reporting that C. globate resistance/dose dependent sensitivity to uconazole but selectively sensitive to other antifungal agents such as nystatin, and amphotericin B, which are similar to the result of this study.Despite this window of hope for non albicans C glabrata susceptibity, resistant strains has increased in patients presenting with candidiasis in recent years ( 46) with increased mortality rates (39).
To understand the emergence of this dose dependent sensitivity in Candida albicans and non-Candida albicans species cohabiting the microecology of the oropharyngeal cavity of HIV patients, Melo et al. (47), found high-frequency transfer of plasmid DNA when pathogenic and food-borne yeasts were grown together despite the fact that many pathogenic yeast species are asexual and therefore not involved in intra-or inter species mating.This high-frequency transfer of plasmid DNA could de ne the observed emergence of dose dependent sensitivity by oropharyngeal Candida albicans, Candida glabrata, Candida sake and Candida norevegensis respectively (Tables 1).
Micro evolutionary high-frequency transfer of plasmid DNA (through cell lyses, or cytoduction) has been proved between C. glabrata and Saccharomyces cerevesiae (48).Although factors that govern the evolutionary revolution of micro-organisms are not yet completely understood, it is thought that environmental and nutritional factors are key to this evolutionary revolution of micro-organisms that can make them change from the original characteristics of their ancestral genealogy (the wild type), to something new (48-49).Microbial evolution tends to remove with time what will not assist its bid to survive in a particular environment and acquire new characters that will assist the organism to manipulate its environment (50) thus accounting for the suggested emergence of dose dependent sensitivity in the reported yeast strains (51).In addition, despite the "asexual" nature of C. glabrata, a potential cryptic sexual life cycle could promote rare interspecies mating with C albicans and other non albicans species of Candida (52).
From (Table 1, g 1-6), C. norvegensis, C glabrata, Saccharomyces cerevisiae and Zygosaccharomyces species isolated from different districts showed a resistant MIC to Itraconazole, and Amphotericin B respectively.Immunosuppression, previous exposure to oral azoles, Clinical, cellular and molecular factors are known to contribute to development of antifungal drug resistance (53)(54).Clinical factors leading to treatment failure of refractory disease may be associated with the patient's immune status (55), pharmacology of the drugs (13), or type of fungal infection (8).Cellular factors could be the result of replacement of a susceptible strain with a more resistant species or the alteration of an endogenous strain (by mutation or gene expression) to a resistant phenotype.
Molecular factors responsible for the resistance phenotype in the cell identi ed has been reported to include over expression of two types of e ux pumps, mutation of the target enzyme, and alteration of other enzymes in the same biosynthetic pathway as the target enzyme (53,11).Generally, azoles and polyenes actions are mainly directed against ergosterol, the major sterol of the fungal plasma membrane, which is analogous to cholesterol in mammalian cells, (13)(14).The dosing schedule may have an effect on the development of resistance (4), since patients treated with intermittent therapy were more likely to develop resistance than those treated continuously.Primary resistance and secondary resistance have been documented in HIV-infected patients (56).Although symptomatic candidiasis develops more commonly due to C. albicans, candidiasis are known to be caused also by non-albicans Candida sp.Fluconazole-resistant species, Candida norvegensis, have recently been reported as pathogens in patients receiving uconazole (41).
The polyenes are known class of amphipathic antifungal drugs that target membranes containing ergosterol forming a channel through which cellular potassium ions, leak and thereby destroying the proton gradient within the membrane (11,57).Amphotericin B has antioxidant effect in vivo, protecting fungal cells against oxidative attack from the host (11,58).Reports of Amphotericin B resistance are limited, but it appears prolonged pre-exposure to azole antifungal drugs and severely immunocompromised patients, especially patients with cancer, are at the highest risk perhaps explained by an alteration of cellular membrane components (59).Amphotericin B-resistant clinical isolates of C. tropicalis have also been found (11,58).Minimum inhibitory concentration (MIC) of tested antifungal agents (Table 1) was signi cantly (p<0.05)dependent on strains of Candida species isolated from oropharyngeal lesions of studied population.Thus, types of oropharyngeal yeast strains present in the oral lesions signi cantly (p<0.05)impacted on the MIC values as well as the e cacy of antifungal agents tested.
There is evidence to suggest that intermittent versus continuous dosing, the amount of drug administered, the length of treatment (27), the immune status of the patient and alterations in the membrane structure or in the sterol-to-phospholipid ratio in the membrane may be associated with resistance (53,11,58).The success or failure of antifungal drug therapy is dependent not only on the drug, but also on the immune status of the host and a combination of azoles and cytokines may be an important therapeutic strategy for resistant fungal infections in immunocompromised individuals.
Susceptibility of C. norvegensis were signi cantly (p<0.05)dependent on types of antifungal used.The depicted different levels of exposure to various antifungal agents tested may be a pointer to emergence of resistant clones of oropharyngeal yeast strains among the HIV infected patients studied.The frequency of diagnosed fungal infections is known to have risen due to increase in the number of immunosuppressed patients resulting from the AIDS epidemic and increase in the frequency of recalcitrant infections to standard antifungal therapy (13)(14), Susceptibility of isolates to antifungals varied and MIC values were signi cantly (p<0.05)dependent on district of sample collection.Thus, despite being treated with same guidelines by clinicians in the same TASO centres, patient's uptake and compliance to treatment guidelines were different, thus depicting different levels of exposure to antifungals agents and probable prescription pattern.Fluconazole-resistant Candida norvegensis, have been reported as pathogens in patients receiving uconazole (41,24,60).It has been suggested that selection for resistant strains occurs with azole treatment but nosocomial transmission and transmission of resistant strains between partners can also occur (53).The polyene are amphipathic drugs which intercalate into membranes containing ergosterol, forming a channel through which cellular components, especially potassium ions, leak and thereby destroying the proton gradient within the membrane (57,24,60).Amphotericin B resistance in Candida spp.can develop in patients previously exposed to azoles (59).Several strains of uconazole-and amphotericin B-resistant C. albicans have been found in HIV-infected patients who have received prolonged courses of azole developed resistance with azoles (59).
Underlying medical conditions of the immunocompromised HIV infected population studied together with type of antifungal used, district of yeast isolation, and strains of yeast isolates signi cantly (p<0.05)impacted on the MIC values obtained in this investigation (Table 1).To justify the role of underlying medical conditions in the healing of oral lesions in the immunocompromised HIV infected population (60), Nube et al (61) summarised speci c risk factors of delayed wound healing to include but not limited to: infection, ischemia, ulcer size, depth, and duration as well as probing to bone (or osteomyelitis), location of ulcer, sensory loss, deformity (and high plantar pressure), advanced age, number of ulcers present and renal disease with ulcer depth (or size), infection, and ischemia listed as the most common risk factors.When there is high-quality treatment protocols and guidelines, 66%-77% of foot ulcers will heal and the remaining group are unlikely to heal, thus supporting the fact that quality of treatment and care impacts on wound healing rate (61).Non healing wounds are prevalent in resource poor settings due to poor treatment protocol while healing or delayed healing wound are more prevalence in adequate resources settings (62).
This study was supported in part by Kampala International University Western Campus and The AIDS Support Organization, Kampala Uganda.The funding bodies played no role in the study apart from the fund provided Authors contributions: This is not applicable as this is a single author manuscript.All other state holders who are not authors are listed in the acknowledgement section

Competing interests
The author declare no competing interests.

Authors' contributions
Not applicable since this is a single author paper.All others who assisted and who are not authors are mentioned in the acknowledgement section

Tables Conclusions
In conclusion Candida albicans remain signi cantly susceptible to tested antifungal agents.Emerging dose dependent susceptibility in non albicans oropharyngeal yeast strains is interesting in this era of increasing treatment failures due to drug resistance.Pattern of resistance recorded may suggest signi cant contribution of yeast in the recurrent or delayed oropharyngeal lesions of HIV population studied.Sustained surveillance with MIC which impacts treatment outcome is recommended.