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Research
Jie Ma
Shanghai Jiaotong University School of Medicine Xinhua Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3776-4429
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This work is licensed under a CC BY 4.0 License. Read Full License
BACKGROUND: It is very important to develop a highly efficient cerebrospinal fluid (CSF) detection system with diagnosis and prediction function, for which the detection of circulating tumor cells (CTCs) in CSF is a good choice. In contrast to the past use of epithelial EpCAM as CTCs separation target, a cytoplasm protein of GFAP antibody was first selected to construct highly-sensitive immunomagnetic liposomes (IMLs). The validation and efficiency of this system in capturing CTCs for brain tumors were measured both in vitro and in vivo. The associations between the numbers of CTCs in patients with their clinical characteristics were further analyzed.
RESULTS: Our data show that CTCs can be successfully isolated from CSF and blood samples from 32 children with brain tumors. The numbers of CTCs in CSF were significantly higher than those in blood. The level of CTCs in CSF was related to the type and location of the tumor rather than its stage. The higher the CTCs number is, the more possibly the patient will suffer from poor prognosis. Genetic testing in GFAP CTC-DNA by sanger sequencing, q-PCR and NGS methods indicated that the isolated CTCs (GFAP+/EGFR+) are the related tumor cell. For example, the high expression of NPR3 gene in CSF CTCs was consistent with that of tumor tissue.
CONCLUSIONS: The results indicated that GFAP-IML CTCs isolation system, combined with an EGFR immunofluorescence assay of antitumor marker, can serve as a brand-new method for the identification of CTCs for brain tumors. Via lumbar puncture, a minimally invasive procedure, this technique may play a significant role in the clinical diagnosis and drug evaluation of brain tumors.
Keywords
Circulating tumor cell, Cytoplasm protein, Liquid biopsy, Tumor diagnosis, Brain tumor
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CURRENT STATUS: Published
View the published article at Journal of Nanobiotechnology.
Version 2
Posted 15 Oct, 2020
No community comments so far
Editorial decision: Accept
On 30 Oct, 2020
Review #1 received
Received 29 Oct, 2020
Reviewer #1 agreed
On 17 Oct, 2020
Reviewers invited
Invitations sent on 16 Oct, 2020
Editor assigned
On 06 Oct, 2020
Submission checks complete
On 05 Oct, 2020
Editor invited
On 05 Oct, 2020
No comments provided
Editorial decision: Major revision
On 03 Sep, 2020
Review #2 received
Received 18 Aug, 2020
Reviewer #1 agreed
On 05 Aug, 2020
Reviewer #2 agreed
On 05 Aug, 2020
Review #1 received
Received 05 Aug, 2020
Reviewers invited
Invitations sent on 04 Aug, 2020
Editor invited
On 27 Jul, 2020
Editor assigned
On 27 Jul, 2020
First submitted
On 26 Jul, 2020
Submission checks complete
On 26 Jul, 2020
Metrics
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HTML Views: ...
Subject Areas
Nanoscience
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A new preprint design is coming!
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See the published version of this preprint at Journal of Nanobiotechnology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Jie Ma
Shanghai Jiaotong University School of Medicine Xinhua Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3776-4429
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Journal of Nanobiotechnology.
Version 2
Posted 15 Oct, 2020
No community comments so far
Editorial decision: Accept
On 30 Oct, 2020
Review #1 received
Received 29 Oct, 2020
Reviewer #1 agreed
On 17 Oct, 2020
Reviewers invited
Invitations sent on 16 Oct, 2020
Editor assigned
On 06 Oct, 2020
Submission checks complete
On 05 Oct, 2020
Editor invited
On 05 Oct, 2020
No comments provided
Editorial decision: Major revision
On 03 Sep, 2020
Review #2 received
Received 18 Aug, 2020
Reviewer #1 agreed
On 05 Aug, 2020
Reviewer #2 agreed
On 05 Aug, 2020
Review #1 received
Received 05 Aug, 2020
Reviewers invited
Invitations sent on 04 Aug, 2020
Editor invited
On 27 Jul, 2020
Editor assigned
On 27 Jul, 2020
First submitted
On 26 Jul, 2020
Submission checks complete
On 26 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Nanoscience
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Journal of Nanobiotechnology.
BACKGROUND: It is very important to develop a highly efficient cerebrospinal fluid (CSF) detection system with diagnosis and prediction function, for which the detection of circulating tumor cells (CTCs) in CSF is a good choice. In contrast to the past use of epithelial EpCAM as CTCs separation target, a cytoplasm protein of GFAP antibody was first selected to construct highly-sensitive immunomagnetic liposomes (IMLs). The validation and efficiency of this system in capturing CTCs for brain tumors were measured both in vitro and in vivo. The associations between the numbers of CTCs in patients with their clinical characteristics were further analyzed.
RESULTS: Our data show that CTCs can be successfully isolated from CSF and blood samples from 32 children with brain tumors. The numbers of CTCs in CSF were significantly higher than those in blood. The level of CTCs in CSF was related to the type and location of the tumor rather than its stage. The higher the CTCs number is, the more possibly the patient will suffer from poor prognosis. Genetic testing in GFAP CTC-DNA by sanger sequencing, q-PCR and NGS methods indicated that the isolated CTCs (GFAP+/EGFR+) are the related tumor cell. For example, the high expression of NPR3 gene in CSF CTCs was consistent with that of tumor tissue.
CONCLUSIONS: The results indicated that GFAP-IML CTCs isolation system, combined with an EGFR immunofluorescence assay of antitumor marker, can serve as a brand-new method for the identification of CTCs for brain tumors. Via lumbar puncture, a minimally invasive procedure, this technique may play a significant role in the clinical diagnosis and drug evaluation of brain tumors.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
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