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Research Article
Gianluca D'Amico
University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6206-7900
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: During the last decades, the spread of the infection with filarial nematodes Dirofilaria spp. in dog’s population in various European countries has been dynamic and rapid. The increased interest of the scientific community as well as the availability of new diagnosis tools, allowed a better knowledge on the biology, morphology and epidemiology of different species of filarial worms. However, in some countries, data are still scarce on this topic. To address some knowledge gaps, we assessed the epidemiological status of different canine filariasis in the Republic of Moldova.
Methods: A total of 120 blood samples were collected between June 2018 and July 2019 from dogs originating from Cahul and Chișinău cities. The samples were microscopically examinated and multiplex PCR was performed to evaluate filarioid species diversity.
Results: Microscopic examination revealed that 12 dogs (10%) were positive for circulating microfilariae. Molecular test showed that 1 dog was positive for Acanthocheilonema reconditum (0.83%), 1 for D. immitis (0.83%), 6 for D. repens (5%), and 4 (3.33%) harboured a coinfection with D. immitis and D. repens.
Conclusions: This study is the first epidemiological survey of filaroid worms in dogs from the Republic of Moldova and the first to show the presence of D. repens and A. reconditum in this species.
Keywords
Dirofilaria spp., Acanthocheilonema spp., vector-borne diseases, zoonosis, dogs, Republic of Moldova
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CURRENT STATUS: Published
View the published article at Parasites & Vectors.
Version 1
Posted 18 May, 2021
No community comments so far
Editorial decision: Major Revision
On 30 May, 2021
Reviews received
Received 16 May, 2021
Reviewers invited
Invitations sent on 16 May, 2021
Editor invited
On 06 May, 2021
First submitted
On 05 May, 2021
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A new preprint design is coming!
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See the published version of this preprint at Parasites & Vectors.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Gianluca D'Amico
University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6206-7900
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Parasites & Vectors.
Version 1
Posted 18 May, 2021
No community comments so far
Editorial decision: Major Revision
On 30 May, 2021
Reviews received
Received 16 May, 2021
Reviewers invited
Invitations sent on 16 May, 2021
Editor invited
On 06 May, 2021
First submitted
On 05 May, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Parasites & Vectors.
Background: During the last decades, the spread of the infection with filarial nematodes Dirofilaria spp. in dog’s population in various European countries has been dynamic and rapid. The increased interest of the scientific community as well as the availability of new diagnosis tools, allowed a better knowledge on the biology, morphology and epidemiology of different species of filarial worms. However, in some countries, data are still scarce on this topic. To address some knowledge gaps, we assessed the epidemiological status of different canine filariasis in the Republic of Moldova.
Methods: A total of 120 blood samples were collected between June 2018 and July 2019 from dogs originating from Cahul and Chișinău cities. The samples were microscopically examinated and multiplex PCR was performed to evaluate filarioid species diversity.
Results: Microscopic examination revealed that 12 dogs (10%) were positive for circulating microfilariae. Molecular test showed that 1 dog was positive for Acanthocheilonema reconditum (0.83%), 1 for D. immitis (0.83%), 6 for D. repens (5%), and 4 (3.33%) harboured a coinfection with D. immitis and D. repens.
Conclusions: This study is the first epidemiological survey of filaroid worms in dogs from the Republic of Moldova and the first to show the presence of D. repens and A. reconditum in this species.
This is a list of supplementary files associated with this preprint. Click to download.
- Graphicalabstract.png
Note: The designations employed and the presentation of the material on this map do not imply the expression of any opinion whatsoever on the part of Research Square concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. This map has been provided by the authors.
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