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Research
Yuehua Gong
the First Hospital of China Medical University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0146-2165
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Cancer stem cells (CSCs) play an important role in drug resistance, recurrence, and metastasis of tumors. Considering the heterogeneity of tumors, this study aimed to explore the key genes regulating stem cells in intestinal-type and diffuse-type gastric cancer (GC).
Methods
RNA-seq data and related clinical information were downloaded from The Cancer Genome Atlas (TCGA). Based on the mRNA expression-based stemness index (mRNAsi), important modules and key genes were identified by weighted co-expression network analysis (WGCNA). The expression of key genes were further verified by Oncomine database.
Results
MRNAsi scores of GC were significantly higher than that of normal tissue. Besides mRNAsi scores of intestinal-type GC (IGC) were significantly higher than that of diffuse-type GC (DGC). WGCNA showed that the brown modules for both types of GC were the most significantly associated with mRNAsi. We screened out 7 and 43 key genes for IGC and DGC, and found that these genes were closely related, respectively. Functional analysis showed the relationship between the key genes confirmed in Oncomine database and fate of cells.
Conclusions
In this study, 7 and 43 genes related to the characteristics of CSCs were identified in IGC and DGC, respectively. These genes were associated with cell cycle and homologous recombination, which could serve as therapeutic targets for the inhibition of stem cells from both types of GC.
Keywords
diffuse-type gastric cancer, intestinal-type gastric cancer, scancer cell stemness, mRNAsi, WGCNA
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Peer Review Timeline
CURRENT STATUS: Published
View the published article at Journal of Translational Medicine.
No community comments so far
Editorial decision: Accept
On 15 Oct, 2020
Review #1 received
Received 14 Oct, 2020
Reviewers invited
Invitations sent on 13 Oct, 2020
Reviewer #1 agreed
On 13 Oct, 2020
Editor assigned
On 10 Oct, 2020
Submission checks complete
On 09 Oct, 2020
Editor invited
On 09 Oct, 2020
Version 1
Posted 04 Aug, 2020
No comments provided
Editorial decision: Major revision
On 07 Sep, 2020
Review #1 received
Received 29 Aug, 2020
Review #3 received
Received 24 Aug, 2020
Review #2 received
Received 24 Aug, 2020
Reviewer #4 agreed
On 19 Aug, 2020
Reviewer #3 agreed
On 14 Aug, 2020
Reviewers invited
Invitations sent on 13 Aug, 2020
Reviewer #1 agreed
On 13 Aug, 2020
Reviewer #2 agreed
On 13 Aug, 2020
Editor assigned
On 11 Aug, 2020
Editor invited
On 10 Aug, 2020
Submission checks complete
On 02 Aug, 2020
First submitted
On 28 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cancer Biology
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Journal of Translational Medicine.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Yuehua Gong
the First Hospital of China Medical University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-0146-2165
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Journal of Translational Medicine.
No community comments so far
Editorial decision: Accept
On 15 Oct, 2020
Review #1 received
Received 14 Oct, 2020
Reviewers invited
Invitations sent on 13 Oct, 2020
Reviewer #1 agreed
On 13 Oct, 2020
Editor assigned
On 10 Oct, 2020
Submission checks complete
On 09 Oct, 2020
Editor invited
On 09 Oct, 2020
Version 1
Posted 04 Aug, 2020
No comments provided
Editorial decision: Major revision
On 07 Sep, 2020
Review #1 received
Received 29 Aug, 2020
Review #3 received
Received 24 Aug, 2020
Review #2 received
Received 24 Aug, 2020
Reviewer #4 agreed
On 19 Aug, 2020
Reviewer #3 agreed
On 14 Aug, 2020
Reviewers invited
Invitations sent on 13 Aug, 2020
Reviewer #1 agreed
On 13 Aug, 2020
Reviewer #2 agreed
On 13 Aug, 2020
Editor assigned
On 11 Aug, 2020
Editor invited
On 10 Aug, 2020
Submission checks complete
On 02 Aug, 2020
First submitted
On 28 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cancer Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Journal of Translational Medicine.
Background
Cancer stem cells (CSCs) play an important role in drug resistance, recurrence, and metastasis of tumors. Considering the heterogeneity of tumors, this study aimed to explore the key genes regulating stem cells in intestinal-type and diffuse-type gastric cancer (GC).
Methods
RNA-seq data and related clinical information were downloaded from The Cancer Genome Atlas (TCGA). Based on the mRNA expression-based stemness index (mRNAsi), important modules and key genes were identified by weighted co-expression network analysis (WGCNA). The expression of key genes were further verified by Oncomine database.
Results
MRNAsi scores of GC were significantly higher than that of normal tissue. Besides mRNAsi scores of intestinal-type GC (IGC) were significantly higher than that of diffuse-type GC (DGC). WGCNA showed that the brown modules for both types of GC were the most significantly associated with mRNAsi. We screened out 7 and 43 key genes for IGC and DGC, and found that these genes were closely related, respectively. Functional analysis showed the relationship between the key genes confirmed in Oncomine database and fate of cells.
Conclusions
In this study, 7 and 43 genes related to the characteristics of CSCs were identified in IGC and DGC, respectively. These genes were associated with cell cycle and homologous recombination, which could serve as therapeutic targets for the inhibition of stem cells from both types of GC.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
This is a list of supplementary files associated with this preprint. Click to download.
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