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Research article
Francesco Tessarolo
University of Trento
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4022-5602
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Peri-implant mucositis and peri-implantitis are biofilm-related diseases causing major concern in oral implantology, requiring complex anti-infective procedures or implant removal. Microbial biosurfactants emerged as new of anti-biofilm agents for coating implantable devices preserving biocompatibility. This study aimed to assess the efficacy of rhamnolipid biosurfactant R89 (R89BS) to reduce Staphylococcus aureus and Staphylococcus epidermidis biofilm formation on titanium.
Methods: R89BS was physically adsorbed on titanium discs (TDs) and the ability of coated TDs to inhibit biofilm formation was evaluated by quantifying biofilm biomass and cell metabolic activity, at different time-points, with respect to uncoated controls. A qualitative analysis of sessile cells was also performed by scanning electron microscopy.
Results: R89BS-coated discs showed no cytotoxic effects on normal lung fibroblasts (MRC5). TDs coated with 4 mg/mL R89BS inhibited the biofilm biomass of S. aureus by 98%, 49% and 10% and of S. epidermidis by 53%, 29%, and 10% at 24, 48 and 72 h respectively. A significant reduction of the biofilm metabolic activity was also documented. The same coating applied on three commercial implant surfaces resulted in a biomass inhibition higher than 90% for S. aureus, and up to 75% for S. epidermidis at 24 h.
Conclusions: R89BS-coating was effective in reducing Staphylococcus biofilm formation at the titanium implant surface. The anti-biofilm action can be obtained on several different commercially available implant surfaces, independently of their surface morphology.
Keywords
dental implants, biofilm, titanium, biosurfactant, Staphylococcus spp.
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CURRENT STATUS: Published
View the published article at BMC Oral Health.
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Editor assigned
On 13 Jan, 2021
Submission checks complete
On 13 Jan, 2021
Editor invited
On 13 Jan, 2021
No comments provided
Review #2 received
Received 07 Jan, 2021
Editorial decision: Minor revision
On 07 Jan, 2021
Reviewer #2 agreed
On 02 Nov, 2020
Review #1 received
Received 27 Oct, 2020
Reviewers invited
Invitations sent on 26 Oct, 2020
Reviewer #1 agreed
On 26 Oct, 2020
Editor assigned
On 22 Sep, 2020
Submission checks complete
On 21 Sep, 2020
Editor invited
On 21 Sep, 2020
Version 1
Posted 10 Aug, 2020
No comments provided
Review #2 received
Received 29 Aug, 2020
Editorial decision: Major revision
On 29 Aug, 2020
Review #1 received
Received 24 Aug, 2020
Reviewers invited
Invitations sent on 07 Aug, 2020
Reviewer #1 agreed
On 07 Aug, 2020
Reviewer #2 agreed
On 07 Aug, 2020
Editor assigned
On 28 Jul, 2020
First submitted
On 27 Jul, 2020
Submission checks complete
On 27 Jul, 2020
Editor invited
On 27 Jul, 2020
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Subject Areas
Dentistry
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A new preprint design is coming!
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See the published version of this preprint at BMC Oral Health.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Francesco Tessarolo
University of Trento
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4022-5602
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Oral Health.
No community comments so far
Editor assigned
On 13 Jan, 2021
Submission checks complete
On 13 Jan, 2021
Editor invited
On 13 Jan, 2021
No comments provided
Review #2 received
Received 07 Jan, 2021
Editorial decision: Minor revision
On 07 Jan, 2021
Reviewer #2 agreed
On 02 Nov, 2020
Review #1 received
Received 27 Oct, 2020
Reviewers invited
Invitations sent on 26 Oct, 2020
Reviewer #1 agreed
On 26 Oct, 2020
Editor assigned
On 22 Sep, 2020
Submission checks complete
On 21 Sep, 2020
Editor invited
On 21 Sep, 2020
Version 1
Posted 10 Aug, 2020
No comments provided
Review #2 received
Received 29 Aug, 2020
Editorial decision: Major revision
On 29 Aug, 2020
Review #1 received
Received 24 Aug, 2020
Reviewers invited
Invitations sent on 07 Aug, 2020
Reviewer #1 agreed
On 07 Aug, 2020
Reviewer #2 agreed
On 07 Aug, 2020
Editor assigned
On 28 Jul, 2020
First submitted
On 27 Jul, 2020
Submission checks complete
On 27 Jul, 2020
Editor invited
On 27 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Dentistry
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Oral Health.
Background: Peri-implant mucositis and peri-implantitis are biofilm-related diseases causing major concern in oral implantology, requiring complex anti-infective procedures or implant removal. Microbial biosurfactants emerged as new of anti-biofilm agents for coating implantable devices preserving biocompatibility. This study aimed to assess the efficacy of rhamnolipid biosurfactant R89 (R89BS) to reduce Staphylococcus aureus and Staphylococcus epidermidis biofilm formation on titanium.
Methods: R89BS was physically adsorbed on titanium discs (TDs) and the ability of coated TDs to inhibit biofilm formation was evaluated by quantifying biofilm biomass and cell metabolic activity, at different time-points, with respect to uncoated controls. A qualitative analysis of sessile cells was also performed by scanning electron microscopy.
Results: R89BS-coated discs showed no cytotoxic effects on normal lung fibroblasts (MRC5). TDs coated with 4 mg/mL R89BS inhibited the biofilm biomass of S. aureus by 98%, 49% and 10% and of S. epidermidis by 53%, 29%, and 10% at 24, 48 and 72 h respectively. A significant reduction of the biofilm metabolic activity was also documented. The same coating applied on three commercial implant surfaces resulted in a biomass inhibition higher than 90% for S. aureus, and up to 75% for S. epidermidis at 24 h.
Conclusions: R89BS-coating was effective in reducing Staphylococcus biofilm formation at the titanium implant surface. The anti-biofilm action can be obtained on several different commercially available implant surfaces, independently of their surface morphology.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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