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Research article
Zhong Liu
Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College
Corresponding Author
License:
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BACKGROUND
Dengue virus (DENV) can be transmitted through blood transfusion. DENV was not screened regularly in Xishuangbanna blood center. This study was conducted in Xishuangbanna blood center with an attempt to develop DENV screening strategies in one of China’s high-incidence areas.
METHODS
Blood samples were collected randomly between June 2019 and August 2019. These samples were first screened for anti-DENV IgG and IgM using enzyme-linked immunosorbent assay (ELISA). All reactive samples and some randomly-chosen non-reactive samples were used to detect DENV RNAs using real-time polymerase-chain-reaction (RT-PCR) assay. After RT-PCR assay, these samples were further tested for soluble nonstructural protein 1 (NS1) using the colloidal gold method. The demographic data of donors were collected.
RESULTS
A total of 2,254 donor samples were collected and tested for anti-DENV IgG and IgM by ELISA between June 2019 and August 2019. ELISA testing revealed that 598 donor samples were anti-DENV IgG and/or IgM reactive, with a serological prevalence of 26.53%. Among all the donor samples, 26 were RT-PCR positive and/or NS1 positive. Moreover, there were significant differences in the prevalence of DENV in terms of occupation (P=0.001), education(P<0.001) and ethnicity (P=0.026).
CONCLUSION
The prevalence of DENV in Xishuangbanna Blood Center was higher than most other blood centers that have implemented DENV donor screening. Our study provides the first-hand data about the prevalence of DENV and allows the development of a screening strategy for clinical use.
Keywords
Dengue, Transfusion-transmitted infection, Prevalence, Donor, Screening strategy
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CURRENT STATUS: Published
View the published article at BMC Infectious Diseases.
No community comments so far
Submission checks complete
On 14 Jan, 2021
Editorial decision: Accept
On 13 Jan, 2021
Version (no preprint)
Posted 11 Jan, 2021
Submission checks complete
On 11 Jan, 2021
Editorial decision: Minor revision
On 10 Jan, 2021
This version was not preprinted
Version 2
Posted 23 Oct, 2020
No comments provided
Editorial decision: Minor revision
On 06 Jan, 2021
Review #2 received
Received 10 Dec, 2020
Reviewer #2 agreed
On 19 Nov, 2020
Reviewers invited
Invitations sent on 17 Nov, 2020
Reviewer #1 agreed
On 17 Nov, 2020
Review #1 received
Received 17 Nov, 2020
Editor assigned
On 13 Oct, 2020
Submission checks complete
On 12 Oct, 2020
Editor invited
On 12 Oct, 2020
No comments provided
Editorial decision: Major revision
On 11 Sep, 2020
Review #2 received
Received 10 Sep, 2020
Review #1 received
Received 07 Sep, 2020
Reviewer #2 agreed
On 20 Aug, 2020
Reviewers invited
Invitations sent on 18 Aug, 2020
Reviewer #1 agreed
On 18 Aug, 2020
Editor assigned
On 23 Jul, 2020
Submission checks complete
On 22 Jul, 2020
Editor invited
On 22 Jul, 2020
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Subject Areas
Infectious Diseases
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A new preprint design is coming!
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See the published version of this preprint at BMC Infectious Diseases.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Zhong Liu
Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Infectious Diseases.
No community comments so far
Submission checks complete
On 14 Jan, 2021
Editorial decision: Accept
On 13 Jan, 2021
Version (no preprint)
Posted 11 Jan, 2021
Submission checks complete
On 11 Jan, 2021
Editorial decision: Minor revision
On 10 Jan, 2021
This version was not preprinted
Version 2
Posted 23 Oct, 2020
No comments provided
Editorial decision: Minor revision
On 06 Jan, 2021
Review #2 received
Received 10 Dec, 2020
Reviewer #2 agreed
On 19 Nov, 2020
Reviewers invited
Invitations sent on 17 Nov, 2020
Reviewer #1 agreed
On 17 Nov, 2020
Review #1 received
Received 17 Nov, 2020
Editor assigned
On 13 Oct, 2020
Submission checks complete
On 12 Oct, 2020
Editor invited
On 12 Oct, 2020
No comments provided
Editorial decision: Major revision
On 11 Sep, 2020
Review #2 received
Received 10 Sep, 2020
Review #1 received
Received 07 Sep, 2020
Reviewer #2 agreed
On 20 Aug, 2020
Reviewers invited
Invitations sent on 18 Aug, 2020
Reviewer #1 agreed
On 18 Aug, 2020
Editor assigned
On 23 Jul, 2020
Submission checks complete
On 22 Jul, 2020
Editor invited
On 22 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Infectious Diseases
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Infectious Diseases.
BACKGROUND
Dengue virus (DENV) can be transmitted through blood transfusion. DENV was not screened regularly in Xishuangbanna blood center. This study was conducted in Xishuangbanna blood center with an attempt to develop DENV screening strategies in one of China’s high-incidence areas.
METHODS
Blood samples were collected randomly between June 2019 and August 2019. These samples were first screened for anti-DENV IgG and IgM using enzyme-linked immunosorbent assay (ELISA). All reactive samples and some randomly-chosen non-reactive samples were used to detect DENV RNAs using real-time polymerase-chain-reaction (RT-PCR) assay. After RT-PCR assay, these samples were further tested for soluble nonstructural protein 1 (NS1) using the colloidal gold method. The demographic data of donors were collected.
RESULTS
A total of 2,254 donor samples were collected and tested for anti-DENV IgG and IgM by ELISA between June 2019 and August 2019. ELISA testing revealed that 598 donor samples were anti-DENV IgG and/or IgM reactive, with a serological prevalence of 26.53%. Among all the donor samples, 26 were RT-PCR positive and/or NS1 positive. Moreover, there were significant differences in the prevalence of DENV in terms of occupation (P=0.001), education(P<0.001) and ethnicity (P=0.026).
CONCLUSION
The prevalence of DENV in Xishuangbanna Blood Center was higher than most other blood centers that have implemented DENV donor screening. Our study provides the first-hand data about the prevalence of DENV and allows the development of a screening strategy for clinical use.
Figure 1
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