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Research article
Zhong Liu
Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College
Corresponding Author
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BACKGROUND Dengue virus (DENV) infection is increasingly common in southern China and can be transmitted through blood transfusion but is not currently part of donor screening throughout the region. We assessed DENV prevalence among donors at the Xishuangbanna Blood Center, Yunnan, to support development of DENV screening strategies.
METHODS Blood samples were collected randomly between June 2019 and August 2019. These were screened for anti-DENV IgG and IgM using enzyme-linked immunosorbent assay (ELISA). Then, all reactive samples and some randomly-chosen non-reactive samples were used to detect DENV RNAs using real-time polymerase-chain-reaction (RT-PCR) assays. After RT-PCR, samples were further tested for soluble nonstructural protein 1 (NS1) using the colloidal gold method. Donors demographics were also collected and assessed.
RESULTS Over the study period, 2,254 donor samples were collected and tested for anti-DENV IgG and IgM by ELISA. This revealed 598 anti-DENV IgG and/or IgM reactive samples, a serological prevalence of 26.53%. Of these, 26 were RT-PCR positive and/or NS1 positive. Significant differences in DENV prevalence were noted by occupation (P=0.001), education (P<0.001), and ethnicity (P=0.026).
CONCLUSION The prevalence of DENV in Xishuangbanna Blood Center was higher than most other blood centers that have implemented DENV donor screening. Our study provides first-hand data about the prevalence of DENV and allows the development of a screening strategy for clinical use.
Keywords
Dengue, Transfusion-transmitted infection, Prevalence, Donor, Screening strategy
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CURRENT STATUS: Published
View the published article at BMC Infectious Diseases.
Version 3
Posted 21 Jan, 2021
No community comments so far
Submission checks complete
On 14 Jan, 2021
Editorial decision: Accept
On 13 Jan, 2021
Version (no preprint)
Posted 11 Jan, 2021
Submission checks complete
On 11 Jan, 2021
Editorial decision: Minor revision
On 10 Jan, 2021
This version was not preprinted
No comments provided
Editorial decision: Minor revision
On 06 Jan, 2021
Review #2 received
Received 10 Dec, 2020
Reviewer #2 agreed
On 19 Nov, 2020
Reviewers invited
Invitations sent on 17 Nov, 2020
Reviewer #1 agreed
On 17 Nov, 2020
Review #1 received
Received 17 Nov, 2020
Editor assigned
On 13 Oct, 2020
Submission checks complete
On 12 Oct, 2020
Editor invited
On 12 Oct, 2020
No comments provided
Editorial decision: Major revision
On 11 Sep, 2020
Review #2 received
Received 10 Sep, 2020
Review #1 received
Received 07 Sep, 2020
Reviewer #2 agreed
On 20 Aug, 2020
Reviewers invited
Invitations sent on 18 Aug, 2020
Reviewer #1 agreed
On 18 Aug, 2020
Editor assigned
On 23 Jul, 2020
Submission checks complete
On 22 Jul, 2020
Editor invited
On 22 Jul, 2020
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Subject Areas
Infectious Diseases
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A new preprint design is coming!
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See the published version of this preprint at BMC Infectious Diseases.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Zhong Liu
Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Infectious Diseases.
Version 3
Posted 21 Jan, 2021
No community comments so far
Submission checks complete
On 14 Jan, 2021
Editorial decision: Accept
On 13 Jan, 2021
Version (no preprint)
Posted 11 Jan, 2021
Submission checks complete
On 11 Jan, 2021
Editorial decision: Minor revision
On 10 Jan, 2021
This version was not preprinted
No comments provided
Editorial decision: Minor revision
On 06 Jan, 2021
Review #2 received
Received 10 Dec, 2020
Reviewer #2 agreed
On 19 Nov, 2020
Reviewers invited
Invitations sent on 17 Nov, 2020
Reviewer #1 agreed
On 17 Nov, 2020
Review #1 received
Received 17 Nov, 2020
Editor assigned
On 13 Oct, 2020
Submission checks complete
On 12 Oct, 2020
Editor invited
On 12 Oct, 2020
No comments provided
Editorial decision: Major revision
On 11 Sep, 2020
Review #2 received
Received 10 Sep, 2020
Review #1 received
Received 07 Sep, 2020
Reviewer #2 agreed
On 20 Aug, 2020
Reviewers invited
Invitations sent on 18 Aug, 2020
Reviewer #1 agreed
On 18 Aug, 2020
Editor assigned
On 23 Jul, 2020
Submission checks complete
On 22 Jul, 2020
Editor invited
On 22 Jul, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Infectious Diseases
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Infectious Diseases.
BACKGROUND Dengue virus (DENV) infection is increasingly common in southern China and can be transmitted through blood transfusion but is not currently part of donor screening throughout the region. We assessed DENV prevalence among donors at the Xishuangbanna Blood Center, Yunnan, to support development of DENV screening strategies.
METHODS Blood samples were collected randomly between June 2019 and August 2019. These were screened for anti-DENV IgG and IgM using enzyme-linked immunosorbent assay (ELISA). Then, all reactive samples and some randomly-chosen non-reactive samples were used to detect DENV RNAs using real-time polymerase-chain-reaction (RT-PCR) assays. After RT-PCR, samples were further tested for soluble nonstructural protein 1 (NS1) using the colloidal gold method. Donors demographics were also collected and assessed.
RESULTS Over the study period, 2,254 donor samples were collected and tested for anti-DENV IgG and IgM by ELISA. This revealed 598 anti-DENV IgG and/or IgM reactive samples, a serological prevalence of 26.53%. Of these, 26 were RT-PCR positive and/or NS1 positive. Significant differences in DENV prevalence were noted by occupation (P=0.001), education (P<0.001), and ethnicity (P=0.026).
CONCLUSION The prevalence of DENV in Xishuangbanna Blood Center was higher than most other blood centers that have implemented DENV donor screening. Our study provides first-hand data about the prevalence of DENV and allows the development of a screening strategy for clinical use.
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