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Research article
Haibo Hu
Nanjing Forestry Univserity
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8486-3975
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Masson pine (Pinus massoniana) is primarily present within subtropical and tropical areas in China, and a number of these regions have a severe deficiency in inorganic phosphate (Pi). As a macronutrient, phosphorus plays a crucial role in plant development. Although several studies have studied the responses of masson pine to Pi starvation at the global level using RNA-Seq and comparative proteomic analyses, the detailed features in the roots that primarily respond to low Pi stress have not yet been studied.
Results: This study examined the masson pine of the response of masson pine roots to a deficiency in Pi. Approximately 1,117 unigenes were shown to respond to Pi-deficiency by differential expression when analyzed using RNA-Seq. A total of 819 and 298 of these transcripts were up- and down-regulated, respectively. The results identified several phosphate transporters (PHT1, PHO88), ABC transporters and metal transporters. In particular, the ethylene response factor (ERF) was the most abundant transcription factor. Analyses of these genes, including gene ontology enrichment and the KEGG pathway analysis, indicated that the metabolic processes are the most enriched under abiotic stresses, including Pi-deficiency.
Conclusions: This study provided abundant transcriptomic information to functionally dissect the response of masson pine roots to a deficiency of Pi, which will provide additional aid to elucidate the biological regulatory mechanisms that the pines use to respond to low Pi stress.
Keywords
Masson pine, Low phosphate, Transcriptome analysis, Gene ontology enrichment
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This is a preprint. It has not completed peer review.
Research article
Haibo Hu
Nanjing Forestry Univserity
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8486-3975
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
View the published article at Acta Physiologiae Plantarum.
Version 1
Posted 17 Sep, 2019
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Acta Physiologiae Plantarum.
Background: Masson pine (Pinus massoniana) is primarily present within subtropical and tropical areas in China, and a number of these regions have a severe deficiency in inorganic phosphate (Pi). As a macronutrient, phosphorus plays a crucial role in plant development. Although several studies have studied the responses of masson pine to Pi starvation at the global level using RNA-Seq and comparative proteomic analyses, the detailed features in the roots that primarily respond to low Pi stress have not yet been studied.
Results: This study examined the masson pine of the response of masson pine roots to a deficiency in Pi. Approximately 1,117 unigenes were shown to respond to Pi-deficiency by differential expression when analyzed using RNA-Seq. A total of 819 and 298 of these transcripts were up- and down-regulated, respectively. The results identified several phosphate transporters (PHT1, PHO88), ABC transporters and metal transporters. In particular, the ethylene response factor (ERF) was the most abundant transcription factor. Analyses of these genes, including gene ontology enrichment and the KEGG pathway analysis, indicated that the metabolic processes are the most enriched under abiotic stresses, including Pi-deficiency.
Conclusions: This study provided abundant transcriptomic information to functionally dissect the response of masson pine roots to a deficiency of Pi, which will provide additional aid to elucidate the biological regulatory mechanisms that the pines use to respond to low Pi stress.
Figure 1
Figure 2
Figure 3
Figure 4
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