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Background: The merging of two divergent genomes during hybridization can result in the remodeling of parental gene expression in hybrids. A molecular basis underling expression change in hybrid is regulatory divergence, which may change with the parental genetic divergence. However, there still no unanimous conclusion for this hypothesis.
Results : Three species of Camellia with a range of genetic divergence and their F 1 hybrids were used to study the effect of parental genetic divergence on gene expression and regulatory patterns in hybrids by RNA-sequencing and allele-specific gene expression analysis. We found that though the proportion of differentially expressed genes (DEGs) between the hybrids and their parents did not increase, a greater proportion of DEGs would be non-additively (especially transgressively) expressed in the hybrids as genomes between the parents become more divergent . In addition, the proportion of genes with significant evidence of cis -regulatory divergence increased , whereas with trans -regulatory divergence decreased with parental genetic divergence.
Conclusions : The discordance within hybrid would intensify as the parents become more divergent, manifesting as more DEGs would be non-additively expressed. Trans -regulatory divergence contributed more to the additively inherited genes than cis , however, its contribution to expression difference would be weakened as cis mutations accumulated over time; and this might be an important reason for that the more divergent the parents are, the greater proportion of DEGs would be non-additively expressed in hybrid.
Keywords
Camellia, allele-specific gene expression, hybridization, transcriptome shock，cis and trans regulation
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Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Genomics.
No community comments so far
Editorial decision: Accept
On 23 Oct, 2019
Review #2 received
Received 22 Oct, 2019
Reviewer #2 agreed
On 21 Oct, 2019
Reviewer #1 agreed
On 19 Oct, 2019
Review #1 received
Received 19 Oct, 2019
Reviewers invited
Invitations sent on 18 Oct, 2019
Editor assigned
On 17 Oct, 2019
Submission checks complete
On 16 Oct, 2019
Editor invited
On 16 Oct, 2019
Version 1
Posted 17 Sep, 2019
No comments provided
Editorial decision: Minor revision
On 04 Oct, 2019
Review #3 received
Received 04 Oct, 2019
Review #1 received
Received 04 Oct, 2019
Review #2 received
Received 29 Sep, 2019
Reviewer #3 agreed
On 19 Sep, 2019
Reviewer #2 agreed
On 18 Sep, 2019
Reviewer #1 agreed
On 16 Sep, 2019
Reviewers invited
Invitations sent on 13 Sep, 2019
Submission checks complete
On 12 Sep, 2019
Editor assigned
On 09 Sep, 2019
Editor invited
On 08 Sep, 2019
First submitted
On 06 Sep, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at BMC Genomics.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Genomics.
No community comments so far
Editorial decision: Accept
On 23 Oct, 2019
Review #2 received
Received 22 Oct, 2019
Reviewer #2 agreed
On 21 Oct, 2019
Reviewer #1 agreed
On 19 Oct, 2019
Review #1 received
Received 19 Oct, 2019
Reviewers invited
Invitations sent on 18 Oct, 2019
Editor assigned
On 17 Oct, 2019
Submission checks complete
On 16 Oct, 2019
Editor invited
On 16 Oct, 2019
Version 1
Posted 17 Sep, 2019
No comments provided
Editorial decision: Minor revision
On 04 Oct, 2019
Review #3 received
Received 04 Oct, 2019
Review #1 received
Received 04 Oct, 2019
Review #2 received
Received 29 Sep, 2019
Reviewer #3 agreed
On 19 Sep, 2019
Reviewer #2 agreed
On 18 Sep, 2019
Reviewer #1 agreed
On 16 Sep, 2019
Reviewers invited
Invitations sent on 13 Sep, 2019
Submission checks complete
On 12 Sep, 2019
Editor assigned
On 09 Sep, 2019
Editor invited
On 08 Sep, 2019
First submitted
On 06 Sep, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Genomics.
Background: The merging of two divergent genomes during hybridization can result in the remodeling of parental gene expression in hybrids. A molecular basis underling expression change in hybrid is regulatory divergence, which may change with the parental genetic divergence. However, there still no unanimous conclusion for this hypothesis.
Results : Three species of Camellia with a range of genetic divergence and their F 1 hybrids were used to study the effect of parental genetic divergence on gene expression and regulatory patterns in hybrids by RNA-sequencing and allele-specific gene expression analysis. We found that though the proportion of differentially expressed genes (DEGs) between the hybrids and their parents did not increase, a greater proportion of DEGs would be non-additively (especially transgressively) expressed in the hybrids as genomes between the parents become more divergent . In addition, the proportion of genes with significant evidence of cis -regulatory divergence increased , whereas with trans -regulatory divergence decreased with parental genetic divergence.
Conclusions : The discordance within hybrid would intensify as the parents become more divergent, manifesting as more DEGs would be non-additively expressed. Trans -regulatory divergence contributed more to the additively inherited genes than cis , however, its contribution to expression difference would be weakened as cis mutations accumulated over time; and this might be an important reason for that the more divergent the parents are, the greater proportion of DEGs would be non-additively expressed in hybrid.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
This is a list of supplementary files associated with this preprint. Click to download.
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