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Research article
Jixiang He
Anhui Academy of Agricultural Sciences
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Chinese soft-shell turtle (Pelodiscus sinensis) is an important commercial species for its high nutritive and medicinal value, but it has been a vulnerable species due to habitat loss. In this study, homologous juvenile turtles were allocated to lake, pond and paddy field to investigate the habitat effects on turtles.
Results
The growth, morphology and gut microbial communities were monitored during the 4 months cultural period. It showed higher growth rate of turtles in paddy field and pond. The appearance, visceral coefficients, gut morphology and microbial communities in turtles were distinct among different habitats, which was mainly attributed to food abundance and living space. The diversity of gut microbial community was obviously higher in turtles from paddy field than pond and lake. Significant differences on dominant phyla, genera and species were found among initial samples and subsequent samples from different habitats. Firmicutes was the most abundant phylum in turtle gut sampled from greenhouse at initial and Proteobacteria was the most abundant phylum after cultivated in different habitats. The functional predictions indicated that both habitat type and sampling time had significant effects on metabolic pathways, especially amino acid and carbohydrate metabolism.
Conclusion
The microbial abundance was different among the habitats and varied within cultural periods. Gut microbes in turtles from paddy field presented high diversity and richness. Rice-turtle coculture was a potential ecological and economic farming mode for its high production and quality of both turtles and rice, which would play important roles in wild turtle protection, food security and paddy field environment improvement.
Keywords
gut microbial variation, diversity, habitat, rice-turtle coculture
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Peer Review Timeline
CURRENT STATUS: Under Review
The most recent version of this article is available here.
No community comments so far
Editorial decision: Major revision
On 24 Jan, 2021
Review #3 received
Received 11 Dec, 2020
Reviewer #3 agreed
On 01 Dec, 2020
Reviewer #2 agreed
On 17 Nov, 2020
Reviewers invited
Invitations sent on 17 Nov, 2020
Reviewer #1 agreed
On 17 Nov, 2020
Review #2 received
Received 17 Nov, 2020
Review #1 received
Received 17 Nov, 2020
Editor assigned
On 14 Oct, 2020
Submission checks complete
On 13 Oct, 2020
Editor invited
On 13 Oct, 2020
Version 1
Posted 26 Aug, 2020
No comments provided
Editorial decision: Major revision
On 21 Sep, 2020
Review #2 received
Received 16 Sep, 2020
Review #1 received
Received 14 Sep, 2020
Review #3 received
Received 07 Sep, 2020
Reviewer #3 agreed
On 26 Aug, 2020
Editor assigned
On 24 Aug, 2020
Reviewers invited
Invitations sent on 24 Aug, 2020
Reviewer #1 agreed
On 24 Aug, 2020
Reviewer #2 agreed
On 24 Aug, 2020
Submission checks complete
On 23 Aug, 2020
Editor invited
On 23 Aug, 2020
Metrics
Comments: 0
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This preprint is under consideration at BMC Microbiology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Jixiang He
Anhui Academy of Agricultural Sciences
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
The most recent version of this article is available here.
No community comments so far
Editorial decision: Major revision
On 24 Jan, 2021
Review #3 received
Received 11 Dec, 2020
Reviewer #3 agreed
On 01 Dec, 2020
Reviewer #2 agreed
On 17 Nov, 2020
Reviewers invited
Invitations sent on 17 Nov, 2020
Reviewer #1 agreed
On 17 Nov, 2020
Review #2 received
Received 17 Nov, 2020
Review #1 received
Received 17 Nov, 2020
Editor assigned
On 14 Oct, 2020
Submission checks complete
On 13 Oct, 2020
Editor invited
On 13 Oct, 2020
Version 1
Posted 26 Aug, 2020
No comments provided
Editorial decision: Major revision
On 21 Sep, 2020
Review #2 received
Received 16 Sep, 2020
Review #1 received
Received 14 Sep, 2020
Review #3 received
Received 07 Sep, 2020
Reviewer #3 agreed
On 26 Aug, 2020
Editor assigned
On 24 Aug, 2020
Reviewers invited
Invitations sent on 24 Aug, 2020
Reviewer #1 agreed
On 24 Aug, 2020
Reviewer #2 agreed
On 24 Aug, 2020
Submission checks complete
On 23 Aug, 2020
Editor invited
On 23 Aug, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The most recent version of this article is available here.
Background
Chinese soft-shell turtle (Pelodiscus sinensis) is an important commercial species for its high nutritive and medicinal value, but it has been a vulnerable species due to habitat loss. In this study, homologous juvenile turtles were allocated to lake, pond and paddy field to investigate the habitat effects on turtles.
Results
The growth, morphology and gut microbial communities were monitored during the 4 months cultural period. It showed higher growth rate of turtles in paddy field and pond. The appearance, visceral coefficients, gut morphology and microbial communities in turtles were distinct among different habitats, which was mainly attributed to food abundance and living space. The diversity of gut microbial community was obviously higher in turtles from paddy field than pond and lake. Significant differences on dominant phyla, genera and species were found among initial samples and subsequent samples from different habitats. Firmicutes was the most abundant phylum in turtle gut sampled from greenhouse at initial and Proteobacteria was the most abundant phylum after cultivated in different habitats. The functional predictions indicated that both habitat type and sampling time had significant effects on metabolic pathways, especially amino acid and carbohydrate metabolism.
Conclusion
The microbial abundance was different among the habitats and varied within cultural periods. Gut microbes in turtles from paddy field presented high diversity and richness. Rice-turtle coculture was a potential ecological and economic farming mode for its high production and quality of both turtles and rice, which would play important roles in wild turtle protection, food security and paddy field environment improvement.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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