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Research
Ke Nie
School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, 510006, P.R.China
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4410-7662
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: In this study, the effects of Xiao-Ban-Xia-Tang (XBXT) formula on cisplatin and 1-phenylbiguanide hydrochloride (1-PBG) induced acute and delayed emesis and gut microbiota were studied in the pica model of rats, compared with ondansetron.
Methods: Two rat models of cisplatin and 1-PBG induced pica were established, the amount of kaolin intake was observed, and the effects of XBXT and ondansetron on the gut microbiota were further studied by 16S rDNA gene analysis.
Results: The results showed that the total intake of kaolin of the rats injected with cisplatin and 1-PBG was significantly increased, and treatment of XBXT and ondansetron could significantly ameliorate the acute and delayed pica induced by cisplatin and 1-PBG. The 16S rDNA gene analysis has shown that the alpha diversity of the gut microbiota of the cisplatin and 1-PBG treated rats was significantly decreased compared with the control group, and ondansetron could further decrease the alpha diversity of the gut microbiota of the rats treated with cisplatin. Ondansetron significantly decreased the relative abundance of Firmicutes and increased the abundance of Bacteroidetes on phylum level in the cisplatin and 1-PBG treated rats, while XBXT only decreased Firmicutes in the cisplatin treated rats.
Conclusions: XBXT was as effective as ondansetron in the treatment of acute and delayed pica induced by cisplatin and 1-PBG in rats. Ondansetron was more likely to cause gut microbiota dysbiosis than XBXT. Our study provided new avenues for the roles and mechanisms of XBXT on the prevention and treatment of CINV.
Keywords
Xiao-Ban-Xia-Tang, pica, gut microbiota, cisplatin, 1-phenylbiguanide hydrochloride, ondansetron
Figure 1
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This is a list of supplementary files associated with this preprint. Click to download.
- SupplementalFigure4.tif
Fig. S4 The relative composition and abundance of the gut microbiota at the bacterial class level. A, B: the relative abundance of the gut microbiota of the 7 groups of rats at the bacterial class level.
- SupplementalFigure3.tif
Fig. S3 The results of HPLC analysis of XBXT, with succinic acid as quality control. HPLC: high performance liquid chromatography; XBXT: Xiao-Ban-Xia-Tang.
- SupplementalFigure2.tif
Fig. S2 The results of HPLC analysis of XBXT, with ephedrine as quality control. HPLC: high performance liquid chromatography; XBXT: Xiao-Ban-Xia-Tang.
- SupplementalFigure1.tif
Fig. S1 The results of HPLC analysis of XBXT, with [6]-gingerol and [6]-shogaol as quality control. HPLC: high performance liquid chromatography; XBXT: Xiao-Ban-Xia-Tang.
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This is a preprint. It has not completed peer review.
Research
Ke Nie
School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, 510006, P.R.China
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4410-7662
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 11 Aug, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Internal Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: In this study, the effects of Xiao-Ban-Xia-Tang (XBXT) formula on cisplatin and 1-phenylbiguanide hydrochloride (1-PBG) induced acute and delayed emesis and gut microbiota were studied in the pica model of rats, compared with ondansetron.
Methods: Two rat models of cisplatin and 1-PBG induced pica were established, the amount of kaolin intake was observed, and the effects of XBXT and ondansetron on the gut microbiota were further studied by 16S rDNA gene analysis.
Results: The results showed that the total intake of kaolin of the rats injected with cisplatin and 1-PBG was significantly increased, and treatment of XBXT and ondansetron could significantly ameliorate the acute and delayed pica induced by cisplatin and 1-PBG. The 16S rDNA gene analysis has shown that the alpha diversity of the gut microbiota of the cisplatin and 1-PBG treated rats was significantly decreased compared with the control group, and ondansetron could further decrease the alpha diversity of the gut microbiota of the rats treated with cisplatin. Ondansetron significantly decreased the relative abundance of Firmicutes and increased the abundance of Bacteroidetes on phylum level in the cisplatin and 1-PBG treated rats, while XBXT only decreased Firmicutes in the cisplatin treated rats.
Conclusions: XBXT was as effective as ondansetron in the treatment of acute and delayed pica induced by cisplatin and 1-PBG in rats. Ondansetron was more likely to cause gut microbiota dysbiosis than XBXT. Our study provided new avenues for the roles and mechanisms of XBXT on the prevention and treatment of CINV.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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