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Research Article
Jing Tian
Training Center for Clinical Skills and Medical staff，General Hospital of Northern Theater Command
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: HIFA-AS1, an antisense transcript of HIF1α gene, is a 652 bp long noncoding RNA (lncRNA) which globally expressed in multiple tissues of animals. Recent evidence indicate d that the HIFA-AS1 was involved in tumorigenesis of several types of cancer, but there were no report s on pancreatic cancer (PC).
Results: In order to investigate whether the HIFA-AS1 could mediate the PC or not, it was overexpressed in a PC cell line (PANC-1), and a series of experiments including cell viability detection, flow cytometry transwell migration, clone formation and wound healing were performed. Functionally, the results indicated that overexpression (OE) of HIFA-AS1 could inhibit proliferation and shift, and promote apoptosis of PC cells. Moreover, to explore underlying molecular mechanism of anti tumorigenic actions of HIFA-AS1 in PC cells, the iTRAQ (isobaric tags for relative and absolute quantification) quantitative proteomics analysis was implemented and the results indicated that OE of HIFA-AS1 globally affected the expression levels of multiple protein associated with metabolism of cancer. Moreover, the network analysis revealed that the most of these differentially expressed proteins (DEPs) were integrated, and severed essential roles in regulatory function.
Conclusions: In summary, HIFA-AS1 may exhibit a potential therapeutic effect on PC, and our study provided useful information in this filed.
Keywords
pancreatic cancer, long non coding RNA, HIFA AS1 , overexpression, molecular mechanism
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The full text of this article is available to read as a PDF.
No competing interests reported.
This is a list of supplementary files associated with this preprint. Click to download.
- Table2.xlsx
Table 2 The all original data from the iTRAQ was analyzed using parameters
- Table3.xlsx
Table 3 The 183 up regulated DEPs were analyzed in OE VS NC
- Table4.xlsx
Table 4 The 155 down regulated DEPs were analyzed in OE VS NC
- supplementary.xlsx
Supplementary . The all original data include d Table 2 Table 3 and Table 4
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CURRENT STATUS: Under Revision
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Posted 10 Jun, 2021
No community comments so far
Editorial decision: Major revision
On 11 Jul, 2021
Reviews received
Received 18 Jun, 2021
Reviewers agreed
On 11 Jun, 2021
Reviewers invited
Invitations sent on 09 Jun, 2021
Editor assigned
On 09 Jun, 2021
Editor invited
On 09 Jun, 2021
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On 09 Jun, 2021
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This preprint is under consideration at BMC Cancer. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Jing Tian
Training Center for Clinical Skills and Medical staff，General Hospital of Northern Theater Command
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 10 Jun, 2021
No community comments so far
Editorial decision: Major revision
On 11 Jul, 2021
Reviews received
Received 18 Jun, 2021
Reviewers agreed
On 11 Jun, 2021
Reviewers invited
Invitations sent on 09 Jun, 2021
Editor assigned
On 09 Jun, 2021
Editor invited
On 09 Jun, 2021
Submission checks complete
On 09 Jun, 2021
First submitted
On 17 May, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: HIFA-AS1, an antisense transcript of HIF1α gene, is a 652 bp long noncoding RNA (lncRNA) which globally expressed in multiple tissues of animals. Recent evidence indicate d that the HIFA-AS1 was involved in tumorigenesis of several types of cancer, but there were no report s on pancreatic cancer (PC).
Results: In order to investigate whether the HIFA-AS1 could mediate the PC or not, it was overexpressed in a PC cell line (PANC-1), and a series of experiments including cell viability detection, flow cytometry transwell migration, clone formation and wound healing were performed. Functionally, the results indicated that overexpression (OE) of HIFA-AS1 could inhibit proliferation and shift, and promote apoptosis of PC cells. Moreover, to explore underlying molecular mechanism of anti tumorigenic actions of HIFA-AS1 in PC cells, the iTRAQ (isobaric tags for relative and absolute quantification) quantitative proteomics analysis was implemented and the results indicated that OE of HIFA-AS1 globally affected the expression levels of multiple protein associated with metabolism of cancer. Moreover, the network analysis revealed that the most of these differentially expressed proteins (DEPs) were integrated, and severed essential roles in regulatory function.
Conclusions: In summary, HIFA-AS1 may exhibit a potential therapeutic effect on PC, and our study provided useful information in this filed.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
The full text of this article is available to read as a PDF.
No competing interests reported.
This is a list of supplementary files associated with this preprint. Click to download.
- Table2.xlsx
Table 2 The all original data from the iTRAQ was analyzed using parameters
- Table3.xlsx
Table 3 The 183 up regulated DEPs were analyzed in OE VS NC
- Table4.xlsx
Table 4 The 155 down regulated DEPs were analyzed in OE VS NC
- supplementary.xlsx
Supplementary . The all original data include d Table 2 Table 3 and Table 4
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